Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
Epididymal sperm protein CRISP1 has the ability to both regulate murine CatSper, a key sperm calcium channel, and interact with egg-binding sites during fertilization. In spite of its relevance for sperm function, Crisp1-/- mice are fertile. Considering that phenotypes can be influenced by the genet...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v99_n2_p_Munoz http://hdl.handle.net/20.500.12110/paper_00063363_v99_n2_p_Munoz |
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paper:paper_00063363_v99_n2_p_Munoz2023-06-08T14:31:10Z Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) Capacitation Fertilization Signal transduction Sperm calcium ion cyclic AMP cyclic AMP dependent protein kinase cysteine rich secretory protein 1 secretory protein unclassified drug acrosome acrosome reaction animal cell animal experiment animal model Article calcium cell level controlled study embryo enzyme phosphorylation female fertility fertilization flow cytometry genetic background in vitro study male mouse nonhuman oocyte phenotype priority journal protein phosphorylation signal transduction sperm spermatozoon capacitation Western blotting zona pellucida Epididymal sperm protein CRISP1 has the ability to both regulate murine CatSper, a key sperm calcium channel, and interact with egg-binding sites during fertilization. In spite of its relevance for sperm function, Crisp1-/- mice are fertile. Considering that phenotypes can be influenced by the genetic background, in the present work mice from the original mixed Crisp1-/- colony (129/SvEv∗C57BL/6) were backcrossed onto the C57BL/6 strain for subsequent analysis of their reproductive phenotype. Whereas fertility and fertilization rates of C57BL/6 Crisp1-/- males did not differ from those reported for mice from the mixed background, several sperm functional parameters were clearly affected by the genetic background. Crisp1-/- sperm from the homogeneous background exhibited defects in both the progesterone-induced acrosome reaction and motility not observed in the mixed background, and normal rather than reduced protein tyrosine phosphorylation. Additional studies revealed a significant decrease in sperm hyperactivation as well as in cAMP and protein kinase A (PKA) substrate phosphorylation levels in sperm from both colonies. The finding that exposure of mutant sperm to a cAMP analog and phosphodiesterase inhibitor overcame the sperm functional defects observed in each colony indicated that a common cAMP-PKA signaling defect led to different phenotypes depending on the genetic background. Altogether, our observations indicate that the phenotype of CRISP1 null males is modulated by the genetic context and reveal new roles for the protein in both the functional events and signaling pathways associated to capacitation. © 2018 Oxford University Press. All rights reserved. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v99_n2_p_Munoz http://hdl.handle.net/20.500.12110/paper_00063363_v99_n2_p_Munoz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Capacitation Fertilization Signal transduction Sperm calcium ion cyclic AMP cyclic AMP dependent protein kinase cysteine rich secretory protein 1 secretory protein unclassified drug acrosome acrosome reaction animal cell animal experiment animal model Article calcium cell level controlled study embryo enzyme phosphorylation female fertility fertilization flow cytometry genetic background in vitro study male mouse nonhuman oocyte phenotype priority journal protein phosphorylation signal transduction sperm spermatozoon capacitation Western blotting zona pellucida |
| spellingShingle |
Capacitation Fertilization Signal transduction Sperm calcium ion cyclic AMP cyclic AMP dependent protein kinase cysteine rich secretory protein 1 secretory protein unclassified drug acrosome acrosome reaction animal cell animal experiment animal model Article calcium cell level controlled study embryo enzyme phosphorylation female fertility fertilization flow cytometry genetic background in vitro study male mouse nonhuman oocyte phenotype priority journal protein phosphorylation signal transduction sperm spermatozoon capacitation Western blotting zona pellucida Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) |
| topic_facet |
Capacitation Fertilization Signal transduction Sperm calcium ion cyclic AMP cyclic AMP dependent protein kinase cysteine rich secretory protein 1 secretory protein unclassified drug acrosome acrosome reaction animal cell animal experiment animal model Article calcium cell level controlled study embryo enzyme phosphorylation female fertility fertilization flow cytometry genetic background in vitro study male mouse nonhuman oocyte phenotype priority journal protein phosphorylation signal transduction sperm spermatozoon capacitation Western blotting zona pellucida |
| description |
Epididymal sperm protein CRISP1 has the ability to both regulate murine CatSper, a key sperm calcium channel, and interact with egg-binding sites during fertilization. In spite of its relevance for sperm function, Crisp1-/- mice are fertile. Considering that phenotypes can be influenced by the genetic background, in the present work mice from the original mixed Crisp1-/- colony (129/SvEv∗C57BL/6) were backcrossed onto the C57BL/6 strain for subsequent analysis of their reproductive phenotype. Whereas fertility and fertilization rates of C57BL/6 Crisp1-/- males did not differ from those reported for mice from the mixed background, several sperm functional parameters were clearly affected by the genetic background. Crisp1-/- sperm from the homogeneous background exhibited defects in both the progesterone-induced acrosome reaction and motility not observed in the mixed background, and normal rather than reduced protein tyrosine phosphorylation. Additional studies revealed a significant decrease in sperm hyperactivation as well as in cAMP and protein kinase A (PKA) substrate phosphorylation levels in sperm from both colonies. The finding that exposure of mutant sperm to a cAMP analog and phosphodiesterase inhibitor overcame the sperm functional defects observed in each colony indicated that a common cAMP-PKA signaling defect led to different phenotypes depending on the genetic background. Altogether, our observations indicate that the phenotype of CRISP1 null males is modulated by the genetic context and reveal new roles for the protein in both the functional events and signaling pathways associated to capacitation. © 2018 Oxford University Press. All rights reserved. |
| title |
Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) |
| title_short |
Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) |
| title_full |
Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) |
| title_fullStr |
Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) |
| title_full_unstemmed |
Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) |
| title_sort |
influence of the genetic background on the reproductive phenotype of mice lacking cysteine-rich secretory protein 1 (crisp1) |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v99_n2_p_Munoz http://hdl.handle.net/20.500.12110/paper_00063363_v99_n2_p_Munoz |
| _version_ |
1768544484267655168 |