Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats
In the present study, we investigated whether vascular endothelial growth factor A (VEGFA) plays a critical intraovarian survival role in gonadotropin-dependent folliculogenesis. The effect of an intrabursal administration of a VEGFA antagonist on follicular development, apoptosis, and levels of pro...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v75_n3_p434_Abramovich http://hdl.handle.net/20.500.12110/paper_00063363_v75_n3_p434_Abramovich |
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paper:paper_00063363_v75_n3_p434_Abramovich2023-06-08T14:31:07Z Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats Angiogenesis Apoptosis Female reproductive tract Follicular development Growth factors Ovary Vascular endothelial growth factor A apoptosis inducing factor apoptosis inhibitor chimeric protein chorionic gonadotropin DNA fragment Fas antigen FAS ligand Fc receptor gonadotropin ligand novormon protein Bax protein bcl 2 protein bcl2l1 protein trap unclassified drug vasculotropin A vasculotropin inhibitor vasculotropin receptor 1 animal experiment animal tissue apoptosis article cell count cell survival chimera controlled study DNA isolation down regulation drug mechanism early antral follicle female gene overexpression nick end labeling nonhuman ovary follicle ovary follicle atresia ovary follicle development ovulation periovulatory follicle preantral follicle prepuberty priority journal protein expression protein family protein function quantitative analysis rat tissue section upregulation Animals Apoptosis bcl-2-Associated X Protein Blotting, Western Chorionic Gonadotropin DNA DNA Fragmentation Electrophoresis, Agar Gel Fas Ligand Protein Fas-Associated Death Domain Protein Female Genes, bcl-1 Genes, bcl-2 In Situ Nick-End Labeling Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Sexual Maturation Vascular Endothelial Growth Factor A In the present study, we investigated whether vascular endothelial growth factor A (VEGFA) plays a critical intraovarian survival role in gonadotropin-dependent folliculogenesis. The effect of an intrabursal administration of a VEGFA antagonist on follicular development, apoptosis, and levels of pro- and antiapoptotic proteins of BCL2 family members (BAX, BCL2, and BCL2L1), as well as of TNFRSF6 (also known as FAS) and FAS ligand (FASLG), was examined. To inhibit VEGFA, a soluble FLT1/Fc Chimera (Trap) was administered to prepubertal eCG-treated rats. Injection of 0.5 μg of Trap per ovary did not change the number of preantral follicles (PFs) or early antral follicles (EAFs); however, it significantly decreased the number of periovulatory follicles 48 h after surgery and significantly increased the number of atretic follicles. No significant differences were found in any stage of the follicles either 12 or 24 h after injection. Cells undergoing DNA fragmentation were quantified by performing TUNEL on ovarian sections. Trap treatment caused a twofold increase in the number of apoptotic cells in EAFs. DNA isolated from antral follicles incubated for 24 h exhibited the typical apoptotic DNA pattern. Follicles obtained from Trap-treated ovaries showed a significant increase in the spontaneous onset of apoptotic DNA fragmentation. The injection of Trap significantly increased the levels of BAX and decreased the levels of BCL2 protein. The ratio of BCL2L1L:BCL2L1s was significantly diminished in follicles obtained from ovaries treated with Trap. No changes in the levels of TNFRSF6 or FASLG were observed after treatment. We concluded that the local inhibition of VEGFA activity appears to produce an increase in ovarian apoptosis through an imbalance among the BCL2 family members, thus leading a larger number of follicles to atresia. © 2006 by the Society for the Study of Reproduction, Inc. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v75_n3_p434_Abramovich http://hdl.handle.net/20.500.12110/paper_00063363_v75_n3_p434_Abramovich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Angiogenesis Apoptosis Female reproductive tract Follicular development Growth factors Ovary Vascular endothelial growth factor A apoptosis inducing factor apoptosis inhibitor chimeric protein chorionic gonadotropin DNA fragment Fas antigen FAS ligand Fc receptor gonadotropin ligand novormon protein Bax protein bcl 2 protein bcl2l1 protein trap unclassified drug vasculotropin A vasculotropin inhibitor vasculotropin receptor 1 animal experiment animal tissue apoptosis article cell count cell survival chimera controlled study DNA isolation down regulation drug mechanism early antral follicle female gene overexpression nick end labeling nonhuman ovary follicle ovary follicle atresia ovary follicle development ovulation periovulatory follicle preantral follicle prepuberty priority journal protein expression protein family protein function quantitative analysis rat tissue section upregulation Animals Apoptosis bcl-2-Associated X Protein Blotting, Western Chorionic Gonadotropin DNA DNA Fragmentation Electrophoresis, Agar Gel Fas Ligand Protein Fas-Associated Death Domain Protein Female Genes, bcl-1 Genes, bcl-2 In Situ Nick-End Labeling Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Sexual Maturation Vascular Endothelial Growth Factor A |
| spellingShingle |
Angiogenesis Apoptosis Female reproductive tract Follicular development Growth factors Ovary Vascular endothelial growth factor A apoptosis inducing factor apoptosis inhibitor chimeric protein chorionic gonadotropin DNA fragment Fas antigen FAS ligand Fc receptor gonadotropin ligand novormon protein Bax protein bcl 2 protein bcl2l1 protein trap unclassified drug vasculotropin A vasculotropin inhibitor vasculotropin receptor 1 animal experiment animal tissue apoptosis article cell count cell survival chimera controlled study DNA isolation down regulation drug mechanism early antral follicle female gene overexpression nick end labeling nonhuman ovary follicle ovary follicle atresia ovary follicle development ovulation periovulatory follicle preantral follicle prepuberty priority journal protein expression protein family protein function quantitative analysis rat tissue section upregulation Animals Apoptosis bcl-2-Associated X Protein Blotting, Western Chorionic Gonadotropin DNA DNA Fragmentation Electrophoresis, Agar Gel Fas Ligand Protein Fas-Associated Death Domain Protein Female Genes, bcl-1 Genes, bcl-2 In Situ Nick-End Labeling Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Sexual Maturation Vascular Endothelial Growth Factor A Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| topic_facet |
Angiogenesis Apoptosis Female reproductive tract Follicular development Growth factors Ovary Vascular endothelial growth factor A apoptosis inducing factor apoptosis inhibitor chimeric protein chorionic gonadotropin DNA fragment Fas antigen FAS ligand Fc receptor gonadotropin ligand novormon protein Bax protein bcl 2 protein bcl2l1 protein trap unclassified drug vasculotropin A vasculotropin inhibitor vasculotropin receptor 1 animal experiment animal tissue apoptosis article cell count cell survival chimera controlled study DNA isolation down regulation drug mechanism early antral follicle female gene overexpression nick end labeling nonhuman ovary follicle ovary follicle atresia ovary follicle development ovulation periovulatory follicle preantral follicle prepuberty priority journal protein expression protein family protein function quantitative analysis rat tissue section upregulation Animals Apoptosis bcl-2-Associated X Protein Blotting, Western Chorionic Gonadotropin DNA DNA Fragmentation Electrophoresis, Agar Gel Fas Ligand Protein Fas-Associated Death Domain Protein Female Genes, bcl-1 Genes, bcl-2 In Situ Nick-End Labeling Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Sexual Maturation Vascular Endothelial Growth Factor A |
| description |
In the present study, we investigated whether vascular endothelial growth factor A (VEGFA) plays a critical intraovarian survival role in gonadotropin-dependent folliculogenesis. The effect of an intrabursal administration of a VEGFA antagonist on follicular development, apoptosis, and levels of pro- and antiapoptotic proteins of BCL2 family members (BAX, BCL2, and BCL2L1), as well as of TNFRSF6 (also known as FAS) and FAS ligand (FASLG), was examined. To inhibit VEGFA, a soluble FLT1/Fc Chimera (Trap) was administered to prepubertal eCG-treated rats. Injection of 0.5 μg of Trap per ovary did not change the number of preantral follicles (PFs) or early antral follicles (EAFs); however, it significantly decreased the number of periovulatory follicles 48 h after surgery and significantly increased the number of atretic follicles. No significant differences were found in any stage of the follicles either 12 or 24 h after injection. Cells undergoing DNA fragmentation were quantified by performing TUNEL on ovarian sections. Trap treatment caused a twofold increase in the number of apoptotic cells in EAFs. DNA isolated from antral follicles incubated for 24 h exhibited the typical apoptotic DNA pattern. Follicles obtained from Trap-treated ovaries showed a significant increase in the spontaneous onset of apoptotic DNA fragmentation. The injection of Trap significantly increased the levels of BAX and decreased the levels of BCL2 protein. The ratio of BCL2L1L:BCL2L1s was significantly diminished in follicles obtained from ovaries treated with Trap. No changes in the levels of TNFRSF6 or FASLG were observed after treatment. We concluded that the local inhibition of VEGFA activity appears to produce an increase in ovarian apoptosis through an imbalance among the BCL2 family members, thus leading a larger number of follicles to atresia. © 2006 by the Society for the Study of Reproduction, Inc. |
| title |
Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| title_short |
Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| title_full |
Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| title_fullStr |
Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| title_full_unstemmed |
Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| title_sort |
effect of a vascular endothelial growth factor (vegf) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v75_n3_p434_Abramovich http://hdl.handle.net/20.500.12110/paper_00063363_v75_n3_p434_Abramovich |
| _version_ |
1768545446152634368 |