Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture
Leptin, the 16-kDa protein product of the obese gene, was originally seen as an adipocyte-derived signaling molecule. Recently, it has been suggested to be involved in some functions during pregnancy, particularly in the placenta. In the present study, we investigated the role of leptin in the secre...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v68_n2_p472_Cameo http://hdl.handle.net/20.500.12110/paper_00063363_v68_n2_p472_Cameo |
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paper:paper_00063363_v68_n2_p472_Cameo2023-06-08T14:31:04Z Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture Cytokines Human chorionic gonadotropin Leptin Progesterone Trophoblast chorionic gonadotropin interleukin 6 leptin messenger RNA progesterone recombinant leptin article cell culture controlled study culture medium cytokine release endocrine function enzyme linked immunosorbent assay gene expression gonadotropin release hormone determination human human cell immunoradiometric assay placenta priority journal progesterone release protein function radioimmunoassay regulatory mechanism reverse transcription polymerase chain reaction trophoblast Cells, Cultured Chorionic Gonadotropin Delivery, Obstetric Female Humans Interleukin-6 Leptin Placenta Pregnancy Progesterone Recombinant Proteins Trophoblasts Mammalia Leptin, the 16-kDa protein product of the obese gene, was originally seen as an adipocyte-derived signaling molecule. Recently, it has been suggested to be involved in some functions during pregnancy, particularly in the placenta. In the present study, we investigated the role of leptin in the secretion of hCG, progesterone, and interleukin-6 (IL-6) by human term trophoblast cells in culture. Placentae were obtained from cesarean sections following uncomplicated pregnancies and used immediately after delivery. Leptin, hCG, progesterone, and IL-6 were measured by ELISA, RIA, and immunoradiometric assay in the cultured media of trophoblast cells cultured for 48 and 96 h. Leptin mRNA expression in these cultures was determined by reverse transcription-polymerase chain reaction. Recombinant human leptin added to primary cultures of human term placental trophoblast cells showed a stimulatory effect on hCG and IL-6 secretion and an inhibitory effect on progesterone secretion. Primary cultures of term trophoblast cells expressed leptin mRNA. All these findings suggest a role for leptin in human placental endocrine function. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v68_n2_p472_Cameo http://hdl.handle.net/20.500.12110/paper_00063363_v68_n2_p472_Cameo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytokines Human chorionic gonadotropin Leptin Progesterone Trophoblast chorionic gonadotropin interleukin 6 leptin messenger RNA progesterone recombinant leptin article cell culture controlled study culture medium cytokine release endocrine function enzyme linked immunosorbent assay gene expression gonadotropin release hormone determination human human cell immunoradiometric assay placenta priority journal progesterone release protein function radioimmunoassay regulatory mechanism reverse transcription polymerase chain reaction trophoblast Cells, Cultured Chorionic Gonadotropin Delivery, Obstetric Female Humans Interleukin-6 Leptin Placenta Pregnancy Progesterone Recombinant Proteins Trophoblasts Mammalia |
| spellingShingle |
Cytokines Human chorionic gonadotropin Leptin Progesterone Trophoblast chorionic gonadotropin interleukin 6 leptin messenger RNA progesterone recombinant leptin article cell culture controlled study culture medium cytokine release endocrine function enzyme linked immunosorbent assay gene expression gonadotropin release hormone determination human human cell immunoradiometric assay placenta priority journal progesterone release protein function radioimmunoassay regulatory mechanism reverse transcription polymerase chain reaction trophoblast Cells, Cultured Chorionic Gonadotropin Delivery, Obstetric Female Humans Interleukin-6 Leptin Placenta Pregnancy Progesterone Recombinant Proteins Trophoblasts Mammalia Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| topic_facet |
Cytokines Human chorionic gonadotropin Leptin Progesterone Trophoblast chorionic gonadotropin interleukin 6 leptin messenger RNA progesterone recombinant leptin article cell culture controlled study culture medium cytokine release endocrine function enzyme linked immunosorbent assay gene expression gonadotropin release hormone determination human human cell immunoradiometric assay placenta priority journal progesterone release protein function radioimmunoassay regulatory mechanism reverse transcription polymerase chain reaction trophoblast Cells, Cultured Chorionic Gonadotropin Delivery, Obstetric Female Humans Interleukin-6 Leptin Placenta Pregnancy Progesterone Recombinant Proteins Trophoblasts Mammalia |
| description |
Leptin, the 16-kDa protein product of the obese gene, was originally seen as an adipocyte-derived signaling molecule. Recently, it has been suggested to be involved in some functions during pregnancy, particularly in the placenta. In the present study, we investigated the role of leptin in the secretion of hCG, progesterone, and interleukin-6 (IL-6) by human term trophoblast cells in culture. Placentae were obtained from cesarean sections following uncomplicated pregnancies and used immediately after delivery. Leptin, hCG, progesterone, and IL-6 were measured by ELISA, RIA, and immunoradiometric assay in the cultured media of trophoblast cells cultured for 48 and 96 h. Leptin mRNA expression in these cultures was determined by reverse transcription-polymerase chain reaction. Recombinant human leptin added to primary cultures of human term placental trophoblast cells showed a stimulatory effect on hCG and IL-6 secretion and an inhibitory effect on progesterone secretion. Primary cultures of term trophoblast cells expressed leptin mRNA. All these findings suggest a role for leptin in human placental endocrine function. |
| title |
Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| title_short |
Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| title_full |
Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| title_fullStr |
Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| title_full_unstemmed |
Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| title_sort |
effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v68_n2_p472_Cameo http://hdl.handle.net/20.500.12110/paper_00063363_v68_n2_p472_Cameo |
| _version_ |
1768543210108354560 |