Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats
The purpose of this study was to evaluate the role of inhibin A in follicular development and apoptosis-related mechanisms in preantral and early antral follicles from prepubertal diethylstilbestrol (DES)-treated rats. Granulosa cells isolated from the ovaries of 23- to 25-day-old rats were cultured...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1989_Vitale http://hdl.handle.net/20.500.12110/paper_00063363_v67_n6_p1989_Vitale |
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paper:paper_00063363_v67_n6_p1989_Vitale2023-06-08T14:31:04Z Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats Apoptosis Follicle Granulosa cells Inhibin diethylstilbestrol DNA fragment estradiol inhibin A progesterone protein Bax protein bcl 2 transforming growth factor beta diethylstilbestrol estradiol follitropin inhibin inhibin A recombinant protein transforming growth factor beta animal cell apoptosis article cell culture cell proliferation drug effect female granulosa cell nonhuman ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal rat animal biosynthesis cell division cytology human immunohistochemistry metabolism nick end labeling ovary follicle Sprague Dawley rat Animalia Animals Apoptosis Cell Division Cells, Cultured Diethylstilbestrol Estradiol Female Follicle Stimulating Hormone Granulosa Cells Humans Immunohistochemistry In Situ Nick-End Labeling Inhibins Ovarian Follicle Progesterone Rats Rats, Sprague-Dawley Recombinant Proteins Transforming Growth Factor beta The purpose of this study was to evaluate the role of inhibin A in follicular development and apoptosis-related mechanisms in preantral and early antral follicles from prepubertal diethylstilbestrol (DES)-treated rats. Granulosa cells isolated from the ovaries of 23- to 25-day-old rats were cultured in serum-free medium containing FSH (20 ng/ml), transforming growth factor β (5 ng/ml), and estradiol (50 ng/ml) in the presence or absence of different concentrations of recombinant human inhibin A. 3H-Thymidine incorporation was decreased in the presence of Inh, but no significant changes were observed in progesterone and estradiol levels in culture medium. An increase in low molecular weight DNA fragmentation indicative of apoptosis and an increase in the levels of Bax protein with no changes in Bcl-2 protein levels were evident in early antral follicles incubated for 24 h with Inh. For each animal, Inh (0.5 μg/ovary) was injected intrabursally in one ovary, and the contralateral ovary served as a control. Ovarian histology revealed an inhibitory effect of Inh treatment on the follicular development induced by DES. At 24 h after Inh injection, the number of preantral follicles was increased compared with controls, whereas the number of early antral follicles was decreased. In addition, in vivo Inh treatment caused an increase in the percentage of apoptotic cells in preantral and early antral follicles. These results suggest that inhibin produced by the dominant follicle may act as a paracrine factor inhibiting the growth of neighboring follicles, thus participating in the mechanism of follicular selection. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1989_Vitale http://hdl.handle.net/20.500.12110/paper_00063363_v67_n6_p1989_Vitale |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Follicle Granulosa cells Inhibin diethylstilbestrol DNA fragment estradiol inhibin A progesterone protein Bax protein bcl 2 transforming growth factor beta diethylstilbestrol estradiol follitropin inhibin inhibin A recombinant protein transforming growth factor beta animal cell apoptosis article cell culture cell proliferation drug effect female granulosa cell nonhuman ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal rat animal biosynthesis cell division cytology human immunohistochemistry metabolism nick end labeling ovary follicle Sprague Dawley rat Animalia Animals Apoptosis Cell Division Cells, Cultured Diethylstilbestrol Estradiol Female Follicle Stimulating Hormone Granulosa Cells Humans Immunohistochemistry In Situ Nick-End Labeling Inhibins Ovarian Follicle Progesterone Rats Rats, Sprague-Dawley Recombinant Proteins Transforming Growth Factor beta |
| spellingShingle |
Apoptosis Follicle Granulosa cells Inhibin diethylstilbestrol DNA fragment estradiol inhibin A progesterone protein Bax protein bcl 2 transforming growth factor beta diethylstilbestrol estradiol follitropin inhibin inhibin A recombinant protein transforming growth factor beta animal cell apoptosis article cell culture cell proliferation drug effect female granulosa cell nonhuman ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal rat animal biosynthesis cell division cytology human immunohistochemistry metabolism nick end labeling ovary follicle Sprague Dawley rat Animalia Animals Apoptosis Cell Division Cells, Cultured Diethylstilbestrol Estradiol Female Follicle Stimulating Hormone Granulosa Cells Humans Immunohistochemistry In Situ Nick-End Labeling Inhibins Ovarian Follicle Progesterone Rats Rats, Sprague-Dawley Recombinant Proteins Transforming Growth Factor beta Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| topic_facet |
Apoptosis Follicle Granulosa cells Inhibin diethylstilbestrol DNA fragment estradiol inhibin A progesterone protein Bax protein bcl 2 transforming growth factor beta diethylstilbestrol estradiol follitropin inhibin inhibin A recombinant protein transforming growth factor beta animal cell apoptosis article cell culture cell proliferation drug effect female granulosa cell nonhuman ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal rat animal biosynthesis cell division cytology human immunohistochemistry metabolism nick end labeling ovary follicle Sprague Dawley rat Animalia Animals Apoptosis Cell Division Cells, Cultured Diethylstilbestrol Estradiol Female Follicle Stimulating Hormone Granulosa Cells Humans Immunohistochemistry In Situ Nick-End Labeling Inhibins Ovarian Follicle Progesterone Rats Rats, Sprague-Dawley Recombinant Proteins Transforming Growth Factor beta |
| description |
The purpose of this study was to evaluate the role of inhibin A in follicular development and apoptosis-related mechanisms in preantral and early antral follicles from prepubertal diethylstilbestrol (DES)-treated rats. Granulosa cells isolated from the ovaries of 23- to 25-day-old rats were cultured in serum-free medium containing FSH (20 ng/ml), transforming growth factor β (5 ng/ml), and estradiol (50 ng/ml) in the presence or absence of different concentrations of recombinant human inhibin A. 3H-Thymidine incorporation was decreased in the presence of Inh, but no significant changes were observed in progesterone and estradiol levels in culture medium. An increase in low molecular weight DNA fragmentation indicative of apoptosis and an increase in the levels of Bax protein with no changes in Bcl-2 protein levels were evident in early antral follicles incubated for 24 h with Inh. For each animal, Inh (0.5 μg/ovary) was injected intrabursally in one ovary, and the contralateral ovary served as a control. Ovarian histology revealed an inhibitory effect of Inh treatment on the follicular development induced by DES. At 24 h after Inh injection, the number of preantral follicles was increased compared with controls, whereas the number of early antral follicles was decreased. In addition, in vivo Inh treatment caused an increase in the percentage of apoptotic cells in preantral and early antral follicles. These results suggest that inhibin produced by the dominant follicle may act as a paracrine factor inhibiting the growth of neighboring follicles, thus participating in the mechanism of follicular selection. |
| title |
Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| title_short |
Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| title_full |
Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| title_fullStr |
Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| title_full_unstemmed |
Inhibin A increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| title_sort |
inhibin a increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1989_Vitale http://hdl.handle.net/20.500.12110/paper_00063363_v67_n6_p1989_Vitale |
| _version_ |
1768544437484388352 |