Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli
A reconstituted UTase/UR-PII-NRII-NRI bicyclic cascade regulated PII uridylylation and NRI phosphorylation in response to glutamine. We examined the sensitivity and robustness of the responses of the individual cycles and of the bicyclic system. The sensitivity of the glutamine response of the upstr...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v51_n45_p9045_Jiang http://hdl.handle.net/20.500.12110/paper_00062960_v51_n45_p9045_Jiang |
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paper:paper_00062960_v51_n45_p9045_Jiang2023-06-08T14:30:47Z Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli Ventura, Alejandra C. Bicyclic systems Glutamine levels Physiological range Protein concentrations Protein level Signal transduction system State response Substrate inhibition Amino acids Escherichia coli Phosphorylation Physiology Proteins Signal transduction Transcription Physiological models bacterial enzyme glutamine nitrogen unclassified drug UTase absolute concentration robustness article bacterial gene concentration (parameters) enzyme activity Escherichia coli genetic transcription nonhuman priority journal protein modification protein phosphorylation signal processing signal transduction transcription regulation uridylation Bacterial Proteins Escherichia coli Escherichia coli Proteins Glutamine Multienzyme Complexes Nitrogen Nucleotidyltransferases Phosphoprotein Phosphatases PII Nitrogen Regulatory Proteins Protein Kinases Protein Multimerization RNA Polymerase Sigma 54 Signal Transduction Transcription Factors Escherichia coli A reconstituted UTase/UR-PII-NRII-NRI bicyclic cascade regulated PII uridylylation and NRI phosphorylation in response to glutamine. We examined the sensitivity and robustness of the responses of the individual cycles and of the bicyclic system. The sensitivity of the glutamine response of the upstream UTase/UR-PII monocycle depended upon the PII concentration, and we show that PII exerted substrate inhibition of the UTase activity of UTase/UR, potentially contributing to this dependence of sensitivity on PII. In the downstream NRII-NRI monocycle, PII controlled NRI phosphorylation state, and the response to PII was hyperbolic at both saturating and unsaturating NRI concentration. As expected from theory, the level of NRI∼P produced by the NRII-NRI monocycle was robust to changes in the NRII or NRI concentrations when NRI was in excess over NRII, as long as the NRII concentration was above a threshold value, an example of absolute concentration robustness (ACR). Because of the parameters of the system, at physiological protein levels and ratios of NRI to NRII, the level of NRI∼P depended upon both protein concentrations. In bicyclic UTase/UR-PII-NRII-NRI systems, the NRI phosphorylation state response to glutamine was always hyperbolic, regardless of the PII concentration or sensitivity of the upstream UTase/UR-PII cycle. In these bicyclic systems, NRI phosphorylation state was only robust to variation in the PII/NRII ratio within a narrow range; when PII was in excess NRI∼P was low, and when NRII was in excess NRI phosphorylation was elevated, throughout the physiological range of glutamine concentrations. Our results show that the bicyclic system produced a graded response of NRI phosphorylation to glutamine under a range of conditions, and that under most conditions the response of NRI phosphorylation state to glutamine levels depended on the concentrations of NRI, NRII, and PII. © 2012 American Chemical Society. Fil:Ventura, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v51_n45_p9045_Jiang http://hdl.handle.net/20.500.12110/paper_00062960_v51_n45_p9045_Jiang |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bicyclic systems Glutamine levels Physiological range Protein concentrations Protein level Signal transduction system State response Substrate inhibition Amino acids Escherichia coli Phosphorylation Physiology Proteins Signal transduction Transcription Physiological models bacterial enzyme glutamine nitrogen unclassified drug UTase absolute concentration robustness article bacterial gene concentration (parameters) enzyme activity Escherichia coli genetic transcription nonhuman priority journal protein modification protein phosphorylation signal processing signal transduction transcription regulation uridylation Bacterial Proteins Escherichia coli Escherichia coli Proteins Glutamine Multienzyme Complexes Nitrogen Nucleotidyltransferases Phosphoprotein Phosphatases PII Nitrogen Regulatory Proteins Protein Kinases Protein Multimerization RNA Polymerase Sigma 54 Signal Transduction Transcription Factors Escherichia coli |
| spellingShingle |
Bicyclic systems Glutamine levels Physiological range Protein concentrations Protein level Signal transduction system State response Substrate inhibition Amino acids Escherichia coli Phosphorylation Physiology Proteins Signal transduction Transcription Physiological models bacterial enzyme glutamine nitrogen unclassified drug UTase absolute concentration robustness article bacterial gene concentration (parameters) enzyme activity Escherichia coli genetic transcription nonhuman priority journal protein modification protein phosphorylation signal processing signal transduction transcription regulation uridylation Bacterial Proteins Escherichia coli Escherichia coli Proteins Glutamine Multienzyme Complexes Nitrogen Nucleotidyltransferases Phosphoprotein Phosphatases PII Nitrogen Regulatory Proteins Protein Kinases Protein Multimerization RNA Polymerase Sigma 54 Signal Transduction Transcription Factors Escherichia coli Ventura, Alejandra C. Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli |
| topic_facet |
Bicyclic systems Glutamine levels Physiological range Protein concentrations Protein level Signal transduction system State response Substrate inhibition Amino acids Escherichia coli Phosphorylation Physiology Proteins Signal transduction Transcription Physiological models bacterial enzyme glutamine nitrogen unclassified drug UTase absolute concentration robustness article bacterial gene concentration (parameters) enzyme activity Escherichia coli genetic transcription nonhuman priority journal protein modification protein phosphorylation signal processing signal transduction transcription regulation uridylation Bacterial Proteins Escherichia coli Escherichia coli Proteins Glutamine Multienzyme Complexes Nitrogen Nucleotidyltransferases Phosphoprotein Phosphatases PII Nitrogen Regulatory Proteins Protein Kinases Protein Multimerization RNA Polymerase Sigma 54 Signal Transduction Transcription Factors Escherichia coli |
| description |
A reconstituted UTase/UR-PII-NRII-NRI bicyclic cascade regulated PII uridylylation and NRI phosphorylation in response to glutamine. We examined the sensitivity and robustness of the responses of the individual cycles and of the bicyclic system. The sensitivity of the glutamine response of the upstream UTase/UR-PII monocycle depended upon the PII concentration, and we show that PII exerted substrate inhibition of the UTase activity of UTase/UR, potentially contributing to this dependence of sensitivity on PII. In the downstream NRII-NRI monocycle, PII controlled NRI phosphorylation state, and the response to PII was hyperbolic at both saturating and unsaturating NRI concentration. As expected from theory, the level of NRI∼P produced by the NRII-NRI monocycle was robust to changes in the NRII or NRI concentrations when NRI was in excess over NRII, as long as the NRII concentration was above a threshold value, an example of absolute concentration robustness (ACR). Because of the parameters of the system, at physiological protein levels and ratios of NRI to NRII, the level of NRI∼P depended upon both protein concentrations. In bicyclic UTase/UR-PII-NRII-NRI systems, the NRI phosphorylation state response to glutamine was always hyperbolic, regardless of the PII concentration or sensitivity of the upstream UTase/UR-PII cycle. In these bicyclic systems, NRI phosphorylation state was only robust to variation in the PII/NRII ratio within a narrow range; when PII was in excess NRI∼P was low, and when NRII was in excess NRI phosphorylation was elevated, throughout the physiological range of glutamine concentrations. Our results show that the bicyclic system produced a graded response of NRI phosphorylation to glutamine under a range of conditions, and that under most conditions the response of NRI phosphorylation state to glutamine levels depended on the concentrations of NRI, NRII, and PII. © 2012 American Chemical Society. |
| author |
Ventura, Alejandra C. |
| author_facet |
Ventura, Alejandra C. |
| author_sort |
Ventura, Alejandra C. |
| title |
Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli |
| title_short |
Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli |
| title_full |
Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli |
| title_fullStr |
Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli |
| title_full_unstemmed |
Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli |
| title_sort |
characterization of the reconstituted utase/ur-pii-nrii-nri bicyclic signal transduction system that controls the transcription of nitrogen-regulated (ntr) genes in escherichia coli |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v51_n45_p9045_Jiang http://hdl.handle.net/20.500.12110/paper_00062960_v51_n45_p9045_Jiang |
| work_keys_str_mv |
AT venturaalejandrac characterizationofthereconstitutedutaseurpiinriinribicyclicsignaltransductionsystemthatcontrolsthetranscriptionofnitrogenregulatedntrgenesinescherichiacoli |
| _version_ |
1768542866224709632 |