7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study
The interaction of 7-aminoactinomycin (7AAMD) with selected DNA sequences (TAGTTA, R5, HP5, and HP1) of different lengths and secondary structures, all containing a 5'-TAGT-3' block, was studied at an ionic strength of 0.02 M and pH 7.7 by means of fluorescence equilibrium and kinetic (sto...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v48_n1_p173_Biver http://hdl.handle.net/20.500.12110/paper_00062960_v48_n1_p173_Biver |
| Aporte de: |
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paper:paper_00062960_v48_n1_p173_Biver |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Binding isotherms Complex dissociation Complex formations Initial rate Secondary structures Atmospheric temperature Diffusers (optical) DNA DNA sequences Genes Ionic strength Nucleic acids Organic acids Stoichiometry Binding sites 7 aminodactinomycin hairpin DNA single stranded DNA unclassified drug article binding affinity binding assay binding kinetics complex formation dissociation DNA sequence DNA structure drug DNA binding drug DNA interaction equilibrium constant fluorescence spectroscopy molecular stability priority journal stoichiometry thermodynamics Base Sequence Dactinomycin DNA Fluorescent Dyes Kinetics Nucleic Acid Conformation Thermodynamics |
| spellingShingle |
Binding isotherms Complex dissociation Complex formations Initial rate Secondary structures Atmospheric temperature Diffusers (optical) DNA DNA sequences Genes Ionic strength Nucleic acids Organic acids Stoichiometry Binding sites 7 aminodactinomycin hairpin DNA single stranded DNA unclassified drug article binding affinity binding assay binding kinetics complex formation dissociation DNA sequence DNA structure drug DNA binding drug DNA interaction equilibrium constant fluorescence spectroscopy molecular stability priority journal stoichiometry thermodynamics Base Sequence Dactinomycin DNA Fluorescent Dyes Kinetics Nucleic Acid Conformation Thermodynamics Jares, Elizabeth Andrea 7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study |
| topic_facet |
Binding isotherms Complex dissociation Complex formations Initial rate Secondary structures Atmospheric temperature Diffusers (optical) DNA DNA sequences Genes Ionic strength Nucleic acids Organic acids Stoichiometry Binding sites 7 aminodactinomycin hairpin DNA single stranded DNA unclassified drug article binding affinity binding assay binding kinetics complex formation dissociation DNA sequence DNA structure drug DNA binding drug DNA interaction equilibrium constant fluorescence spectroscopy molecular stability priority journal stoichiometry thermodynamics Base Sequence Dactinomycin DNA Fluorescent Dyes Kinetics Nucleic Acid Conformation Thermodynamics |
| description |
The interaction of 7-aminoactinomycin (7AAMD) with selected DNA sequences (TAGTTA, R5, HP5, and HP1) of different lengths and secondary structures, all containing a 5'-TAGT-3' block, was studied at an ionic strength of 0.02 M and pH 7.7 by means of fluorescence equilibrium and kinetic (stopped-flow) measurements. Both approaches indicated that the antibiotic binds strongly to both the single-stranded and hairpin (HP1) structures, although the sequences lacked the canonical GpC sites favored by actinomycin. Binding isotherms and initial rate analyses revealed that the binding stoichiometry was 1:1 in all cases. While the single-stranded sequences displayed a simple monoexponential kinetic behavior, the binding of 7AAMD to HP1 at <30°C was biphasic and could be rationalized in terms of a sequential formation of two isomeric bound forms or alternatively in terms of an ssHP1-hpHP1 equilibrium, with both HP1 forms reacting with 7AAMD. The rates of complex dissociation induced by the detergent SDS were also measured. After correction of the kinetic traces for spurious effects that can be attributed to the SDS, monoexponential traces were obtained, with relaxation times in agreement with the kinetics of complex formation. © 2009 American Chemical Society. |
| author |
Jares, Elizabeth Andrea |
| author_facet |
Jares, Elizabeth Andrea |
| author_sort |
Jares, Elizabeth Andrea |
| title |
7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study |
| title_short |
7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study |
| title_full |
7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study |
| title_fullStr |
7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study |
| title_full_unstemmed |
7-aminoactinomycin binding to DNA sequences lacking GpC sites: A thermodynamic and kinetic study |
| title_sort |
7-aminoactinomycin binding to dna sequences lacking gpc sites: a thermodynamic and kinetic study |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v48_n1_p173_Biver http://hdl.handle.net/20.500.12110/paper_00062960_v48_n1_p173_Biver |
| work_keys_str_mv |
AT jareselizabethandrea 7aminoactinomycinbindingtodnasequenceslackinggpcsitesathermodynamicandkineticstudy |
| bdutipo_str |
Repositorios |
| _version_ |
1764820556500172800 |