α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR
The α9 and α10 nicotinic acetylcholine receptor (nAChR) subunits assemble to form the α9α10 nAChR subtype. This receptor is believed to mediate cholinergic synaptic transmission between efferent olivocochlear fibers and the hair cells of the cochlea. In addition α9 and/or α10 expression has been des...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v45_n5_p1511_Ellison http://hdl.handle.net/20.500.12110/paper_00062960_v45_n5_p1511_Ellison |
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paper:paper_00062960_v45_n5_p1511_Ellison2023-06-08T14:30:40Z α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR Amino acids Biochemistry Neurology Skin Tissue Conotoxin Dorsal root ganglion neurons Lymphocytes Nicotinic acetylcholine receptor (nAChR) Cells alpha conotoxin RgIA alpha10 nicotinic receptor alpha9 alpha10 nicotinic receptor alpha9 nicotinic receptor amino acid conotoxin nicotinic receptor nicotinic receptor blocking agent receptor subtype receptor subunit unclassified drug animal cell animal tissue article carboxy terminal sequence cholinergic transmission cochlea controlled study Conus regius efferent nerve hair cell hypophysis pars tuberalis keratinocyte lymphocyte nonhuman priority journal protein assembly protein expression rat receptor blocking snail spinal ganglion toxin structure Animals Base Sequence Cloning, Molecular Conotoxins Gene Expression Regulation Hair Cells, Inner Molecular Sequence Data Oocytes Polymerase Chain Reaction Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Species Specificity Xenopus Animalia Conus regius Gastropoda The α9 and α10 nicotinic acetylcholine receptor (nAChR) subunits assemble to form the α9α10 nAChR subtype. This receptor is believed to mediate cholinergic synaptic transmission between efferent olivocochlear fibers and the hair cells of the cochlea. In addition α9 and/or α10 expression has been described in dorsal root ganglion neurons, lymphocytes, skin keratinocytes, and the pars tuberalis of the pituitary. Specific antagonists that selectively block the α9α10 channel could be valuable tools for elucidating its role in these diverse tissues. This study describes a novel α-conotoxin from the Western Atlantic species Conus regius, α-conotoxin RgIA (α-RgIA), that is a subtype specific blocker of the α9α10 nAChR. α-RgIA belongs to the α4/3 subfamily of the α-conotoxin family; sequence and subtype specificity comparisons between α-RgIA and previously characterized α4/3 toxins indicate that the amino acids in the C-terminal half of α-RgIA are responsible for its preferential inhibition of the α9α10 nAChR subtype. © 2006 American Chemical Society. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v45_n5_p1511_Ellison http://hdl.handle.net/20.500.12110/paper_00062960_v45_n5_p1511_Ellison |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amino acids Biochemistry Neurology Skin Tissue Conotoxin Dorsal root ganglion neurons Lymphocytes Nicotinic acetylcholine receptor (nAChR) Cells alpha conotoxin RgIA alpha10 nicotinic receptor alpha9 alpha10 nicotinic receptor alpha9 nicotinic receptor amino acid conotoxin nicotinic receptor nicotinic receptor blocking agent receptor subtype receptor subunit unclassified drug animal cell animal tissue article carboxy terminal sequence cholinergic transmission cochlea controlled study Conus regius efferent nerve hair cell hypophysis pars tuberalis keratinocyte lymphocyte nonhuman priority journal protein assembly protein expression rat receptor blocking snail spinal ganglion toxin structure Animals Base Sequence Cloning, Molecular Conotoxins Gene Expression Regulation Hair Cells, Inner Molecular Sequence Data Oocytes Polymerase Chain Reaction Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Species Specificity Xenopus Animalia Conus regius Gastropoda |
| spellingShingle |
Amino acids Biochemistry Neurology Skin Tissue Conotoxin Dorsal root ganglion neurons Lymphocytes Nicotinic acetylcholine receptor (nAChR) Cells alpha conotoxin RgIA alpha10 nicotinic receptor alpha9 alpha10 nicotinic receptor alpha9 nicotinic receptor amino acid conotoxin nicotinic receptor nicotinic receptor blocking agent receptor subtype receptor subunit unclassified drug animal cell animal tissue article carboxy terminal sequence cholinergic transmission cochlea controlled study Conus regius efferent nerve hair cell hypophysis pars tuberalis keratinocyte lymphocyte nonhuman priority journal protein assembly protein expression rat receptor blocking snail spinal ganglion toxin structure Animals Base Sequence Cloning, Molecular Conotoxins Gene Expression Regulation Hair Cells, Inner Molecular Sequence Data Oocytes Polymerase Chain Reaction Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Species Specificity Xenopus Animalia Conus regius Gastropoda α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR |
| topic_facet |
Amino acids Biochemistry Neurology Skin Tissue Conotoxin Dorsal root ganglion neurons Lymphocytes Nicotinic acetylcholine receptor (nAChR) Cells alpha conotoxin RgIA alpha10 nicotinic receptor alpha9 alpha10 nicotinic receptor alpha9 nicotinic receptor amino acid conotoxin nicotinic receptor nicotinic receptor blocking agent receptor subtype receptor subunit unclassified drug animal cell animal tissue article carboxy terminal sequence cholinergic transmission cochlea controlled study Conus regius efferent nerve hair cell hypophysis pars tuberalis keratinocyte lymphocyte nonhuman priority journal protein assembly protein expression rat receptor blocking snail spinal ganglion toxin structure Animals Base Sequence Cloning, Molecular Conotoxins Gene Expression Regulation Hair Cells, Inner Molecular Sequence Data Oocytes Polymerase Chain Reaction Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Species Specificity Xenopus Animalia Conus regius Gastropoda |
| description |
The α9 and α10 nicotinic acetylcholine receptor (nAChR) subunits assemble to form the α9α10 nAChR subtype. This receptor is believed to mediate cholinergic synaptic transmission between efferent olivocochlear fibers and the hair cells of the cochlea. In addition α9 and/or α10 expression has been described in dorsal root ganglion neurons, lymphocytes, skin keratinocytes, and the pars tuberalis of the pituitary. Specific antagonists that selectively block the α9α10 channel could be valuable tools for elucidating its role in these diverse tissues. This study describes a novel α-conotoxin from the Western Atlantic species Conus regius, α-conotoxin RgIA (α-RgIA), that is a subtype specific blocker of the α9α10 nAChR. α-RgIA belongs to the α4/3 subfamily of the α-conotoxin family; sequence and subtype specificity comparisons between α-RgIA and previously characterized α4/3 toxins indicate that the amino acids in the C-terminal half of α-RgIA are responsible for its preferential inhibition of the α9α10 nAChR subtype. © 2006 American Chemical Society. |
| title |
α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR |
| title_short |
α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR |
| title_full |
α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR |
| title_fullStr |
α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR |
| title_full_unstemmed |
α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR |
| title_sort |
α-rgia: a novel conotoxin that specifically and potently blocks the α9α10 nachr |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v45_n5_p1511_Ellison http://hdl.handle.net/20.500.12110/paper_00062960_v45_n5_p1511_Ellison |
| _version_ |
1768545813915500544 |