Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells
The aryl hydrocarbon receptor (AHR) mediates toxic responses to environmental contaminants and plays pivotal physiological roles in various biological processes as well, particularly in ovarian function. It is well documented that expression and function of the AHR is negatively regulated by transfo...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062952_v76_n9_p1165_Bussmann http://hdl.handle.net/20.500.12110/paper_00062952_v76_n9_p1165_Bussmann |
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paper:paper_00062952_v76_n9_p1165_Bussmann2023-06-08T14:30:35Z Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells Bussmann, Ursula A. Barañao, José Lino S. β-Naphthoflavone Aryl hydrocarbon receptor Granulosa cells Proliferation Transforming growth factor-β aromatic hydrocarbon aromatic hydrocarbon receptor ligand transforming growth factor beta animal cell animal experiment article cell proliferation female gene expression granulosa cell mitogenesis nonhuman priority journal protein expression protein function protein interaction rat signal transduction transcription regulation Animals Cells, Cultured Female Granulosa Cells Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Sheep Signal Transduction Swine Transforming Growth Factor beta The aryl hydrocarbon receptor (AHR) mediates toxic responses to environmental contaminants and plays pivotal physiological roles in various biological processes as well, particularly in ovarian function. It is well documented that expression and function of the AHR is negatively regulated by transforming growth factor-β (TGF-β) in many cell types. In addition, several studies indicate that AHR activity inhibits TGF-β expression and function in some systems. However, the interplay between these two signals is highly dependent upon the cell type being studied, precluding a generalization about the outcome of such interaction. Therefore, the goal of the present study was to determine the effect of TGF-β on AHR expression and activation in granulosa cells, an ovarian cell type where the growth factor is mitogenic and AHR activation has been associated with promotion of proliferation as well. In addition, we conducted experiments aimed at evaluating the effect of AHR ligands on TGF-β action in our system. Results presented herein demonstrate that AHR expression is not regulated by TGF-β in rat granulosa cells, neither at the mRNA level nor at the protein level. Moreover, we find that the growth factor does not alter the transcriptional function of the AHR. Conversely, we show that activation of AHR by an agonist deregulates TGF-β function in granulosa cells, inhibiting its transcriptional activity and its mitogenic action. The described one-sided interplay between TGF-β and AHR signaling pathway may help provide a mechanistic explanation to some of the physiological outcomes of AHR or TGF-β activation in granulosa cells. © 2008 Elsevier Inc. All rights reserved. Fil:Bussmann, U.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062952_v76_n9_p1165_Bussmann http://hdl.handle.net/20.500.12110/paper_00062952_v76_n9_p1165_Bussmann |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Naphthoflavone Aryl hydrocarbon receptor Granulosa cells Proliferation Transforming growth factor-β aromatic hydrocarbon aromatic hydrocarbon receptor ligand transforming growth factor beta animal cell animal experiment article cell proliferation female gene expression granulosa cell mitogenesis nonhuman priority journal protein expression protein function protein interaction rat signal transduction transcription regulation Animals Cells, Cultured Female Granulosa Cells Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Sheep Signal Transduction Swine Transforming Growth Factor beta |
| spellingShingle |
β-Naphthoflavone Aryl hydrocarbon receptor Granulosa cells Proliferation Transforming growth factor-β aromatic hydrocarbon aromatic hydrocarbon receptor ligand transforming growth factor beta animal cell animal experiment article cell proliferation female gene expression granulosa cell mitogenesis nonhuman priority journal protein expression protein function protein interaction rat signal transduction transcription regulation Animals Cells, Cultured Female Granulosa Cells Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Sheep Signal Transduction Swine Transforming Growth Factor beta Bussmann, Ursula A. Barañao, José Lino S. Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells |
| topic_facet |
β-Naphthoflavone Aryl hydrocarbon receptor Granulosa cells Proliferation Transforming growth factor-β aromatic hydrocarbon aromatic hydrocarbon receptor ligand transforming growth factor beta animal cell animal experiment article cell proliferation female gene expression granulosa cell mitogenesis nonhuman priority journal protein expression protein function protein interaction rat signal transduction transcription regulation Animals Cells, Cultured Female Granulosa Cells Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Sheep Signal Transduction Swine Transforming Growth Factor beta |
| description |
The aryl hydrocarbon receptor (AHR) mediates toxic responses to environmental contaminants and plays pivotal physiological roles in various biological processes as well, particularly in ovarian function. It is well documented that expression and function of the AHR is negatively regulated by transforming growth factor-β (TGF-β) in many cell types. In addition, several studies indicate that AHR activity inhibits TGF-β expression and function in some systems. However, the interplay between these two signals is highly dependent upon the cell type being studied, precluding a generalization about the outcome of such interaction. Therefore, the goal of the present study was to determine the effect of TGF-β on AHR expression and activation in granulosa cells, an ovarian cell type where the growth factor is mitogenic and AHR activation has been associated with promotion of proliferation as well. In addition, we conducted experiments aimed at evaluating the effect of AHR ligands on TGF-β action in our system. Results presented herein demonstrate that AHR expression is not regulated by TGF-β in rat granulosa cells, neither at the mRNA level nor at the protein level. Moreover, we find that the growth factor does not alter the transcriptional function of the AHR. Conversely, we show that activation of AHR by an agonist deregulates TGF-β function in granulosa cells, inhibiting its transcriptional activity and its mitogenic action. The described one-sided interplay between TGF-β and AHR signaling pathway may help provide a mechanistic explanation to some of the physiological outcomes of AHR or TGF-β activation in granulosa cells. © 2008 Elsevier Inc. All rights reserved. |
| author |
Bussmann, Ursula A. Barañao, José Lino S. |
| author_facet |
Bussmann, Ursula A. Barañao, José Lino S. |
| author_sort |
Bussmann, Ursula A. |
| title |
Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells |
| title_short |
Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells |
| title_full |
Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells |
| title_fullStr |
Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells |
| title_full_unstemmed |
Interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: Evidence of an asymmetrical relationship in rat granulosa cells |
| title_sort |
interaction between the aryl hydrocarbon receptor and transforming growth factor-β signaling pathways: evidence of an asymmetrical relationship in rat granulosa cells |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062952_v76_n9_p1165_Bussmann http://hdl.handle.net/20.500.12110/paper_00062952_v76_n9_p1165_Bussmann |
| work_keys_str_mv |
AT bussmannursulaa interactionbetweenthearylhydrocarbonreceptorandtransforminggrowthfactorbsignalingpathwaysevidenceofanasymmetricalrelationshipinratgranulosacells AT baranaojoselinos interactionbetweenthearylhydrocarbonreceptorandtransforminggrowthfactorbsignalingpathwaysevidenceofanasymmetricalrelationshipinratgranulosacells |
| _version_ |
1768545076493942784 |