Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation

In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, an...

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Autor principal: Scolaro, Luis Alberto
Publicado: 2014
Materias:
Bone morphogenetic proteins
Differentiation
Measles virus
Osteoblasts
Otosclerosis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v_n_p_AyalaPena
http://hdl.handle.net/20.500.12110/paper_0006291X_v_n_p_AyalaPena
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_0006291X_v_n_p_AyalaPena2023-06-08T14:30:28Z Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation Scolaro, Luis Alberto Bone morphogenetic proteins Differentiation Measles virus Osteoblasts Otosclerosis In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins (BMPs) 1-4-5-7 in OBCs revealed higher values (2-75-fold of increment) for infected cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium prior to infection influenced the level of stimulation induced by MeV. Furthermore, treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas treatment with 10 μM or 100 μM ATPγS diminished MeV multiplication. In addition, increments of osteogenic differentiation markers induced by MeV infection were not modified either by treatment with Ly294002 or ATPγS. These data provide the first evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic differentiation, a key feature in bone pathogenic processes like otosclerosis. © 2014 Elsevier Inc. All rights reserved. Fil:Scolaro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v_n_p_AyalaPena http://hdl.handle.net/20.500.12110/paper_0006291X_v_n_p_AyalaPena
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bone morphogenetic proteins
Differentiation
Measles virus
Osteoblasts
Otosclerosis
spellingShingle Bone morphogenetic proteins
Differentiation
Measles virus
Osteoblasts
Otosclerosis
Scolaro, Luis Alberto
Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
topic_facet Bone morphogenetic proteins
Differentiation
Measles virus
Osteoblasts
Otosclerosis
description In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins (BMPs) 1-4-5-7 in OBCs revealed higher values (2-75-fold of increment) for infected cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium prior to infection influenced the level of stimulation induced by MeV. Furthermore, treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas treatment with 10 μM or 100 μM ATPγS diminished MeV multiplication. In addition, increments of osteogenic differentiation markers induced by MeV infection were not modified either by treatment with Ly294002 or ATPγS. These data provide the first evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic differentiation, a key feature in bone pathogenic processes like otosclerosis. © 2014 Elsevier Inc. All rights reserved.
author Scolaro, Luis Alberto
author_facet Scolaro, Luis Alberto
author_sort Scolaro, Luis Alberto
title Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_short Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_full Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_fullStr Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_full_unstemmed Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
title_sort experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v_n_p_AyalaPena
http://hdl.handle.net/20.500.12110/paper_0006291X_v_n_p_AyalaPena
work_keys_str_mv AT scolaroluisalberto experimentalinvitroinfectionofratosteoblastswithmeaslesvirusstimulatesosteogenicdifferentiation
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