Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity...
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| Autores principales: | , |
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1977
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v75_n4_p1045_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati |
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| Sumario: | Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity for the binding of formyl-methionyl-tRNA to ribosomes, induced by the trinucleotide AUG. The analysis of polypeptides formed showed that extracts from polyamine-depleted and supplemented cells are both able to synthesize complete protein chains. © 1977. |
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