Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli
Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity...
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1977
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v75_n4_p1045_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati |
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paper:paper_0006291X_v75_n4_p1045_Algranati2023-06-08T14:30:26Z Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli Algranati, Israel David Goldemberg, Sara Hebe messenger rna polyamine bacteriophage escherichia coli formylmethionyl transfer ribonucleic acid in vitro study microorganism rna translation theoretical study Coliphages Escherichia coli Kinetics Magnesium Polyamines Putrescine Ribosomes RNA, Messenger Translation, Genetic Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity for the binding of formyl-methionyl-tRNA to ribosomes, induced by the trinucleotide AUG. The analysis of polypeptides formed showed that extracts from polyamine-depleted and supplemented cells are both able to synthesize complete protein chains. © 1977. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v75_n4_p1045_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
messenger rna polyamine bacteriophage escherichia coli formylmethionyl transfer ribonucleic acid in vitro study microorganism rna translation theoretical study Coliphages Escherichia coli Kinetics Magnesium Polyamines Putrescine Ribosomes RNA, Messenger Translation, Genetic |
spellingShingle |
messenger rna polyamine bacteriophage escherichia coli formylmethionyl transfer ribonucleic acid in vitro study microorganism rna translation theoretical study Coliphages Escherichia coli Kinetics Magnesium Polyamines Putrescine Ribosomes RNA, Messenger Translation, Genetic Algranati, Israel David Goldemberg, Sara Hebe Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli |
topic_facet |
messenger rna polyamine bacteriophage escherichia coli formylmethionyl transfer ribonucleic acid in vitro study microorganism rna translation theoretical study Coliphages Escherichia coli Kinetics Magnesium Polyamines Putrescine Ribosomes RNA, Messenger Translation, Genetic |
description |
Cell-free extracts of polyamine-deficient and supplemented bacteria were used to study the translation of MS 2 bacteriophage RNA. The decreased synthetic activity observed with preparations of starved cells depends on a deficient initiation of polypeptide synthesis, as indicated by a lower capacity for the binding of formyl-methionyl-tRNA to ribosomes, induced by the trinucleotide AUG. The analysis of polypeptides formed showed that extracts from polyamine-depleted and supplemented cells are both able to synthesize complete protein chains. © 1977. |
author |
Algranati, Israel David Goldemberg, Sara Hebe |
author_facet |
Algranati, Israel David Goldemberg, Sara Hebe |
author_sort |
Algranati, Israel David |
title |
Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli |
title_short |
Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli |
title_full |
Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli |
title_fullStr |
Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli |
title_full_unstemmed |
Translation of natural mRNA in cell-free systems from a polyamine-requiring mutant of Escherichia coli |
title_sort |
translation of natural mrna in cell-free systems from a polyamine-requiring mutant of escherichia coli |
publishDate |
1977 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v75_n4_p1045_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v75_n4_p1045_Algranati |
work_keys_str_mv |
AT algranatiisraeldavid translationofnaturalmrnaincellfreesystemsfromapolyaminerequiringmutantofescherichiacoli AT goldembergsarahebe translationofnaturalmrnaincellfreesystemsfromapolyaminerequiringmutantofescherichiacoli |
_version_ |
1768543926631792640 |