Glycosyl transfer to an acceptor lipid from insects
Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were eq...
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1975
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v66_n4_p1201_QuesadaAllue http://hdl.handle.net/20.500.12110/paper_0006291X_v66_n4_p1201_QuesadaAllue |
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paper:paper_0006291X_v66_n4_p1201_QuesadaAllue2023-06-08T14:30:25Z Glycosyl transfer to an acceptor lipid from insects Quesada Allué, Luis Alberto Belocopitow, Enrique lipid uridine diphosphate glucose uridine diphosphate n acetylglucosamine arthropod in vitro study liver microsome rat theoretical study Acetylglucosamine Animal Chromatography, Thin Layer Diptera Glucose Glycolipids Insects Phospholipids Rats Species Specificity Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were equal to those of dolichol monophosphate. The glycosylated compounds were acid labile. Treatment with alkali of the acetylglucosaminyl compound produced a substance that migrated like a hexose phosphate on electrophoresis and that liberated acetylglucosamine on treatment with alkaline phosphatase. The behaviour of the insect glucosylated lipid on thin-layer chromatography and its stability to phenol were similar to dolichol monophosphate glucose and different from ficaprenyl monophosphate glucose. It is concluded that the insect glycosyl acceptor lipid is an α saturated polyprenyl phosphate. © 1975. Fil:Quesada Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Belocopitow, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1975 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v66_n4_p1201_QuesadaAllue http://hdl.handle.net/20.500.12110/paper_0006291X_v66_n4_p1201_QuesadaAllue |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
lipid uridine diphosphate glucose uridine diphosphate n acetylglucosamine arthropod in vitro study liver microsome rat theoretical study Acetylglucosamine Animal Chromatography, Thin Layer Diptera Glucose Glycolipids Insects Phospholipids Rats Species Specificity |
spellingShingle |
lipid uridine diphosphate glucose uridine diphosphate n acetylglucosamine arthropod in vitro study liver microsome rat theoretical study Acetylglucosamine Animal Chromatography, Thin Layer Diptera Glucose Glycolipids Insects Phospholipids Rats Species Specificity Quesada Allué, Luis Alberto Belocopitow, Enrique Glycosyl transfer to an acceptor lipid from insects |
topic_facet |
lipid uridine diphosphate glucose uridine diphosphate n acetylglucosamine arthropod in vitro study liver microsome rat theoretical study Acetylglucosamine Animal Chromatography, Thin Layer Diptera Glucose Glycolipids Insects Phospholipids Rats Species Specificity |
description |
Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were equal to those of dolichol monophosphate. The glycosylated compounds were acid labile. Treatment with alkali of the acetylglucosaminyl compound produced a substance that migrated like a hexose phosphate on electrophoresis and that liberated acetylglucosamine on treatment with alkaline phosphatase. The behaviour of the insect glucosylated lipid on thin-layer chromatography and its stability to phenol were similar to dolichol monophosphate glucose and different from ficaprenyl monophosphate glucose. It is concluded that the insect glycosyl acceptor lipid is an α saturated polyprenyl phosphate. © 1975. |
author |
Quesada Allué, Luis Alberto Belocopitow, Enrique |
author_facet |
Quesada Allué, Luis Alberto Belocopitow, Enrique |
author_sort |
Quesada Allué, Luis Alberto |
title |
Glycosyl transfer to an acceptor lipid from insects |
title_short |
Glycosyl transfer to an acceptor lipid from insects |
title_full |
Glycosyl transfer to an acceptor lipid from insects |
title_fullStr |
Glycosyl transfer to an acceptor lipid from insects |
title_full_unstemmed |
Glycosyl transfer to an acceptor lipid from insects |
title_sort |
glycosyl transfer to an acceptor lipid from insects |
publishDate |
1975 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v66_n4_p1201_QuesadaAllue http://hdl.handle.net/20.500.12110/paper_0006291X_v66_n4_p1201_QuesadaAllue |
work_keys_str_mv |
AT quesadaallueluisalberto glycosyltransfertoanacceptorlipidfrominsects AT belocopitowenrique glycosyltransfertoanacceptorlipidfrominsects |
_version_ |
1768544575997083648 |