DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes

The presence and some properties of DNA polymerases isolated from normal human lymphocytes, non stimulated and stimulated by phytohemagglutinin, are described. In the non stimulated lymphocytes two cytoplasmic DNA polymerases are found, one eluting from DEAE cellulose at 0.07 M NaCl (CIn) and the ot...

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Publicado:	1974
Materias:	1,10 phenanthroline bovine serum albumin dithiothreitol dna dna polymerase enzyme magnesium chloride manganese phytohemagglutinin potassium chloride cytoplasm deoxynucleoside triphosphate human in vitro study lymphocyte lymphocyte transformation theoretical study thymus Animal Cattle Cell Nucleus Chromatography, DEAE-Cellulose Cytoplasm Dithiothreitol DNA Nucleotidyltransferases Human Hydrogen-Ion Concentration Lectins Lymphocyte Activation Lymphocytes Magnesium Manganese Phenanthrolines Serum Albumin, Bovine Sodium Chloride Stimulation, Chemical Tritium
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v60_n3_p983_Fridlender http://hdl.handle.net/20.500.12110/paper_0006291X_v60_n3_p983_Fridlender
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v60_n3_p983_Fridlender
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spelling	paper:paper_0006291X_v60_n3_p983_Fridlender2023-06-08T14:30:24Z DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes 1,10 phenanthroline bovine serum albumin dithiothreitol dna dna polymerase enzyme magnesium chloride manganese phytohemagglutinin potassium chloride cytoplasm deoxynucleoside triphosphate human in vitro study lymphocyte lymphocyte transformation theoretical study thymus Animal Cattle Cell Nucleus Chromatography, DEAE-Cellulose Cytoplasm Dithiothreitol DNA Nucleotidyltransferases Human Hydrogen-Ion Concentration Lectins Lymphocyte Activation Lymphocytes Magnesium Manganese Phenanthrolines Serum Albumin, Bovine Sodium Chloride Stimulation, Chemical Tritium The presence and some properties of DNA polymerases isolated from normal human lymphocytes, non stimulated and stimulated by phytohemagglutinin, are described. In the non stimulated lymphocytes two cytoplasmic DNA polymerases are found, one eluting from DEAE cellulose at 0.07 M NaCl (CIn) and the other at 0.13 M NaCl (CIIn). In the nuclear soluble fraction only one enzyme activity is found (NIn) which does not adsorb to DEAE cellulose. In the cytoplasm of stimulated lymphocytes only one enzyme activity is detected (CIs) which elutes from DEAE cellulose at 0.12 M NaCl. The nuclear soluble fraction contains two activities, NIs, which does not adsorb to DEAE cellulose, and NIIs, which elutes from DEAE cellulose at 0.07 M NaCl. Some properties of the different enzymes are described which indicate that NIn and NIs enzymes are clearly different from the others. © 1974. 1974 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v60_n3_p983_Fridlender http://hdl.handle.net/20.500.12110/paper_0006291X_v60_n3_p983_Fridlender
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	1,10 phenanthroline bovine serum albumin dithiothreitol dna dna polymerase enzyme magnesium chloride manganese phytohemagglutinin potassium chloride cytoplasm deoxynucleoside triphosphate human in vitro study lymphocyte lymphocyte transformation theoretical study thymus Animal Cattle Cell Nucleus Chromatography, DEAE-Cellulose Cytoplasm Dithiothreitol DNA Nucleotidyltransferases Human Hydrogen-Ion Concentration Lectins Lymphocyte Activation Lymphocytes Magnesium Manganese Phenanthrolines Serum Albumin, Bovine Sodium Chloride Stimulation, Chemical Tritium
spellingShingle	1,10 phenanthroline bovine serum albumin dithiothreitol dna dna polymerase enzyme magnesium chloride manganese phytohemagglutinin potassium chloride cytoplasm deoxynucleoside triphosphate human in vitro study lymphocyte lymphocyte transformation theoretical study thymus Animal Cattle Cell Nucleus Chromatography, DEAE-Cellulose Cytoplasm Dithiothreitol DNA Nucleotidyltransferases Human Hydrogen-Ion Concentration Lectins Lymphocyte Activation Lymphocytes Magnesium Manganese Phenanthrolines Serum Albumin, Bovine Sodium Chloride Stimulation, Chemical Tritium DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
topic_facet	1,10 phenanthroline bovine serum albumin dithiothreitol dna dna polymerase enzyme magnesium chloride manganese phytohemagglutinin potassium chloride cytoplasm deoxynucleoside triphosphate human in vitro study lymphocyte lymphocyte transformation theoretical study thymus Animal Cattle Cell Nucleus Chromatography, DEAE-Cellulose Cytoplasm Dithiothreitol DNA Nucleotidyltransferases Human Hydrogen-Ion Concentration Lectins Lymphocyte Activation Lymphocytes Magnesium Manganese Phenanthrolines Serum Albumin, Bovine Sodium Chloride Stimulation, Chemical Tritium
description	The presence and some properties of DNA polymerases isolated from normal human lymphocytes, non stimulated and stimulated by phytohemagglutinin, are described. In the non stimulated lymphocytes two cytoplasmic DNA polymerases are found, one eluting from DEAE cellulose at 0.07 M NaCl (CIn) and the other at 0.13 M NaCl (CIIn). In the nuclear soluble fraction only one enzyme activity is found (NIn) which does not adsorb to DEAE cellulose. In the cytoplasm of stimulated lymphocytes only one enzyme activity is detected (CIs) which elutes from DEAE cellulose at 0.12 M NaCl. The nuclear soluble fraction contains two activities, NIs, which does not adsorb to DEAE cellulose, and NIIs, which elutes from DEAE cellulose at 0.07 M NaCl. Some properties of the different enzymes are described which indicate that NIn and NIs enzymes are clearly different from the others. © 1974.
title	DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
title_short	DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
title_full	DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
title_fullStr	DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
title_full_unstemmed	DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
title_sort	dna polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
publishDate	1974
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v60_n3_p983_Fridlender http://hdl.handle.net/20.500.12110/paper_0006291X_v60_n3_p983_Fridlender
_version_	1768546657010450432

DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes

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