Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum
Parasites of the genus Plasmodium responsible for Malaria are obligate intracellular pathogens residing in mammalian red blood cells, hepatocytes, or mosquito midgut epithelial cells. Regarding that detailed knowledge on the sphingolipid biosynthetic pathway of the apicomplexan protozoan parasites i...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v497_n4_p1082_Pinero http://hdl.handle.net/20.500.12110/paper_0006291X_v497_n4_p1082_Pinero |
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paper:paper_0006291X_v497_n4_p1082_Pinero2023-06-08T14:30:22Z Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum Glucosylceramide synthase Glycosphingolipids Plasmodium falciparum Tamoxifen ceramide glucosyltransferase glycosphingolipid phosphatidylinositol sphingolipid sphingomyelin sphingomyelin synthase tamoxifen unclassified drug glycosphingolipid inositolphosphoceramides phosphatidylinositol sphingolipid tamoxifen Apicomplexa Article controlled study drug effect enzyme inhibition high performance liquid chromatography life cycle stage lipid storage lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman Plasmodium falciparum priority journal reversed phase liquid chromatography schizont sphingolipid metabolism thin layer chromatography trophozoite Apicomplexa biosynthesis drug effects erythrocyte life cycle stage mass spectrometry metabolism parasitology Plasmodium falciparum protozoal infection Apicomplexa Biosynthetic Pathways Chromatography, Reverse-Phase Erythrocytes Glycosphingolipids Life Cycle Stages Mass Spectrometry Phosphatidylinositols Plasmodium falciparum Protozoan Infections Sphingolipids Tamoxifen Parasites of the genus Plasmodium responsible for Malaria are obligate intracellular pathogens residing in mammalian red blood cells, hepatocytes, or mosquito midgut epithelial cells. Regarding that detailed knowledge on the sphingolipid biosynthetic pathway of the apicomplexan protozoan parasites is scarce, different stages of Plasmodium falciparum were treated with tamoxifen in order to evaluate the effects of this drug on the glycosphingolipid biosynthesis. Thin layer chromatography, High performance reverse phase chromatography and UV-MALDI-TOF mass spectrometry were the tools used for the analysis. In the ring forms, the increase of NBD-phosphatidyl inositol biosynthesis was notorious but differences at NBD-GlcCer levels were undetectable. In trophozoite forms, an abrupt decrease of NBD-acylated GlcDHCer and NBD-GlcDHCer in addition to an increase of NBD-PC biosynthesis was observed. On the contrary, in schizonts, tamoxifen seems not to be producing substantial changes in lipid biosynthesis. Our findings indicate that in this parasite, tamoxifen is exerting an inhibitory action on Glucosylceramidesynthase and sphingomyelin synthase levels. Moreover, regarding that Plasmodium does not biosynthesize inositolphosphoceramides, the accumulation of phosphatidylinositol should indicate an inhibitory action on glycosylinositol phospholipid synthesis. © 2018 Elsevier Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v497_n4_p1082_Pinero http://hdl.handle.net/20.500.12110/paper_0006291X_v497_n4_p1082_Pinero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Glucosylceramide synthase Glycosphingolipids Plasmodium falciparum Tamoxifen ceramide glucosyltransferase glycosphingolipid phosphatidylinositol sphingolipid sphingomyelin sphingomyelin synthase tamoxifen unclassified drug glycosphingolipid inositolphosphoceramides phosphatidylinositol sphingolipid tamoxifen Apicomplexa Article controlled study drug effect enzyme inhibition high performance liquid chromatography life cycle stage lipid storage lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman Plasmodium falciparum priority journal reversed phase liquid chromatography schizont sphingolipid metabolism thin layer chromatography trophozoite Apicomplexa biosynthesis drug effects erythrocyte life cycle stage mass spectrometry metabolism parasitology Plasmodium falciparum protozoal infection Apicomplexa Biosynthetic Pathways Chromatography, Reverse-Phase Erythrocytes Glycosphingolipids Life Cycle Stages Mass Spectrometry Phosphatidylinositols Plasmodium falciparum Protozoan Infections Sphingolipids Tamoxifen |
| spellingShingle |
Glucosylceramide synthase Glycosphingolipids Plasmodium falciparum Tamoxifen ceramide glucosyltransferase glycosphingolipid phosphatidylinositol sphingolipid sphingomyelin sphingomyelin synthase tamoxifen unclassified drug glycosphingolipid inositolphosphoceramides phosphatidylinositol sphingolipid tamoxifen Apicomplexa Article controlled study drug effect enzyme inhibition high performance liquid chromatography life cycle stage lipid storage lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman Plasmodium falciparum priority journal reversed phase liquid chromatography schizont sphingolipid metabolism thin layer chromatography trophozoite Apicomplexa biosynthesis drug effects erythrocyte life cycle stage mass spectrometry metabolism parasitology Plasmodium falciparum protozoal infection Apicomplexa Biosynthetic Pathways Chromatography, Reverse-Phase Erythrocytes Glycosphingolipids Life Cycle Stages Mass Spectrometry Phosphatidylinositols Plasmodium falciparum Protozoan Infections Sphingolipids Tamoxifen Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum |
| topic_facet |
Glucosylceramide synthase Glycosphingolipids Plasmodium falciparum Tamoxifen ceramide glucosyltransferase glycosphingolipid phosphatidylinositol sphingolipid sphingomyelin sphingomyelin synthase tamoxifen unclassified drug glycosphingolipid inositolphosphoceramides phosphatidylinositol sphingolipid tamoxifen Apicomplexa Article controlled study drug effect enzyme inhibition high performance liquid chromatography life cycle stage lipid storage lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman Plasmodium falciparum priority journal reversed phase liquid chromatography schizont sphingolipid metabolism thin layer chromatography trophozoite Apicomplexa biosynthesis drug effects erythrocyte life cycle stage mass spectrometry metabolism parasitology Plasmodium falciparum protozoal infection Apicomplexa Biosynthetic Pathways Chromatography, Reverse-Phase Erythrocytes Glycosphingolipids Life Cycle Stages Mass Spectrometry Phosphatidylinositols Plasmodium falciparum Protozoan Infections Sphingolipids Tamoxifen |
| description |
Parasites of the genus Plasmodium responsible for Malaria are obligate intracellular pathogens residing in mammalian red blood cells, hepatocytes, or mosquito midgut epithelial cells. Regarding that detailed knowledge on the sphingolipid biosynthetic pathway of the apicomplexan protozoan parasites is scarce, different stages of Plasmodium falciparum were treated with tamoxifen in order to evaluate the effects of this drug on the glycosphingolipid biosynthesis. Thin layer chromatography, High performance reverse phase chromatography and UV-MALDI-TOF mass spectrometry were the tools used for the analysis. In the ring forms, the increase of NBD-phosphatidyl inositol biosynthesis was notorious but differences at NBD-GlcCer levels were undetectable. In trophozoite forms, an abrupt decrease of NBD-acylated GlcDHCer and NBD-GlcDHCer in addition to an increase of NBD-PC biosynthesis was observed. On the contrary, in schizonts, tamoxifen seems not to be producing substantial changes in lipid biosynthesis. Our findings indicate that in this parasite, tamoxifen is exerting an inhibitory action on Glucosylceramidesynthase and sphingomyelin synthase levels. Moreover, regarding that Plasmodium does not biosynthesize inositolphosphoceramides, the accumulation of phosphatidylinositol should indicate an inhibitory action on glycosylinositol phospholipid synthesis. © 2018 Elsevier Inc. |
| title |
Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum |
| title_short |
Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum |
| title_full |
Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum |
| title_fullStr |
Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum |
| title_full_unstemmed |
Effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa Plasmodium falciparum |
| title_sort |
effect of tamoxifen on the sphingolipid biosynthetic pathway in the different intraerythrocytic stages of the apicomplexa plasmodium falciparum |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v497_n4_p1082_Pinero http://hdl.handle.net/20.500.12110/paper_0006291X_v497_n4_p1082_Pinero |
| _version_ |
1768544437121581056 |