Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing i...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n2_p252_Solari http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari |
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paper:paper_0006291X_v410_n2_p252_Solari2023-06-08T14:30:19Z Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium Losino, Noelia Ivana Luzzani, Carlos Daniel Waisman, Ariel Questa, María Barañao, José Lino S. Guberman, Alejandra Sonia BGC BGC-CM CM Conditioned medium Culture conditions ESC GC Induced pluripotent stem cell IPSC LIF MEF MESC Pluripotency Self-renewal SM animal cell article cell differentiation cell type controlled study fibroblast gene expression granulosa cell immunofluorescence in vitro study in vivo study Lentivirinae marker gene mouse nonhuman pluripotent stem cell priority journal Animals Cattle Cell Culture Techniques Cell Line Culture Media, Conditioned Female Granulosa Cells Induced Pluripotent Stem Cells Mice Octamer Transcription Factor-3 Regeneration Bovinae Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest.In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency. © 2011 Elsevier Inc. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Questa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n2_p252_Solari http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
BGC BGC-CM CM Conditioned medium Culture conditions ESC GC Induced pluripotent stem cell IPSC LIF MEF MESC Pluripotency Self-renewal SM animal cell article cell differentiation cell type controlled study fibroblast gene expression granulosa cell immunofluorescence in vitro study in vivo study Lentivirinae marker gene mouse nonhuman pluripotent stem cell priority journal Animals Cattle Cell Culture Techniques Cell Line Culture Media, Conditioned Female Granulosa Cells Induced Pluripotent Stem Cells Mice Octamer Transcription Factor-3 Regeneration Bovinae |
spellingShingle |
BGC BGC-CM CM Conditioned medium Culture conditions ESC GC Induced pluripotent stem cell IPSC LIF MEF MESC Pluripotency Self-renewal SM animal cell article cell differentiation cell type controlled study fibroblast gene expression granulosa cell immunofluorescence in vitro study in vivo study Lentivirinae marker gene mouse nonhuman pluripotent stem cell priority journal Animals Cattle Cell Culture Techniques Cell Line Culture Media, Conditioned Female Granulosa Cells Induced Pluripotent Stem Cells Mice Octamer Transcription Factor-3 Regeneration Bovinae Losino, Noelia Ivana Luzzani, Carlos Daniel Waisman, Ariel Questa, María Barañao, José Lino S. Guberman, Alejandra Sonia Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
topic_facet |
BGC BGC-CM CM Conditioned medium Culture conditions ESC GC Induced pluripotent stem cell IPSC LIF MEF MESC Pluripotency Self-renewal SM animal cell article cell differentiation cell type controlled study fibroblast gene expression granulosa cell immunofluorescence in vitro study in vivo study Lentivirinae marker gene mouse nonhuman pluripotent stem cell priority journal Animals Cattle Cell Culture Techniques Cell Line Culture Media, Conditioned Female Granulosa Cells Induced Pluripotent Stem Cells Mice Octamer Transcription Factor-3 Regeneration Bovinae |
description |
Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest.In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency. © 2011 Elsevier Inc. |
author |
Losino, Noelia Ivana Luzzani, Carlos Daniel Waisman, Ariel Questa, María Barañao, José Lino S. Guberman, Alejandra Sonia |
author_facet |
Losino, Noelia Ivana Luzzani, Carlos Daniel Waisman, Ariel Questa, María Barañao, José Lino S. Guberman, Alejandra Sonia |
author_sort |
Losino, Noelia Ivana |
title |
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
title_short |
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
title_full |
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
title_fullStr |
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
title_full_unstemmed |
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
title_sort |
induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
publishDate |
2011 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n2_p252_Solari http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari |
work_keys_str_mv |
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1768541638138789888 |