STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line

Gene regulation mediated by STAT factors has been implicated in cellular functions with relevance to a variety of processes. Particularly, STAT5 and STAT3 play a crucial role in mammary epithelium displaying reciprocal activation kinetics during pregnancy, lactation and involution. Here, we show tha...

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Autores principales:	Rodríguez Granillo, Agustina, Boffi, Juan Carlos, Barañao, José Lino S., Kordon, Edith Claudia, Pecci, Adali, Guberman, Alejandra Sonia
Publicado:	2007
Materias:	Gene expression HC11 Involution LIF Mammary epithelium Prolactin Signal transduction STAT3 STAT5 leukemia inhibitory factor prolactin STAT3 protein STAT5 protein article breast epithelium controlled study gene control gene expression regulation gene repression human human cell involution priority journal promoter region protein phosphorylation signal transduction transcription regulation Animals bcl-X Protein CCAAT-Enhancer-Binding Protein-delta Dexamethasone Epithelium Insulin Leukemia Inhibitory Factor Mammary Glands, Animal Mice Phosphorylation Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos STAT3 Transcription Factor STAT5 Transcription Factor
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v356_n3_p727_RodriguezGranillo http://hdl.handle.net/20.500.12110/paper_0006291X_v356_n3_p727_RodriguezGranillo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v356_n3_p727_RodriguezGranillo
record_format	dspace
spelling	paper:paper_0006291X_v356_n3_p727_RodriguezGranillo2023-06-08T14:30:16Z STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line Rodríguez Granillo, Agustina Boffi, Juan Carlos Barañao, José Lino S. Kordon, Edith Claudia Pecci, Adali Guberman, Alejandra Sonia Gene expression HC11 Involution LIF Mammary epithelium Prolactin Signal transduction STAT3 STAT5 leukemia inhibitory factor prolactin STAT3 protein STAT5 protein article breast epithelium controlled study gene control gene expression regulation gene repression human human cell involution priority journal promoter region protein phosphorylation signal transduction transcription regulation Animals bcl-X Protein CCAAT-Enhancer-Binding Protein-delta Dexamethasone Epithelium Insulin Leukemia Inhibitory Factor Mammary Glands, Animal Mice Phosphorylation Prolactin Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos STAT3 Transcription Factor STAT5 Transcription Factor Gene regulation mediated by STAT factors has been implicated in cellular functions with relevance to a variety of processes. Particularly, STAT5 and STAT3 play a crucial role in mammary epithelium displaying reciprocal activation kinetics during pregnancy, lactation and involution. Here, we show that LIF treatment of mammary epithelial HC11 cells reduces the phosphorylation levels and transcriptional activity of p-STAT5 in correlation with STAT3 phosphorylation. We have also found that STAT5 activity is negatively modulated by this cytokine, both on a gene whose expression is induced, as well as on a promoter repressed by STAT5. Besides, our results show that lactogenic hormones increase LIF effect on gene induction without modifying STAT3 phosphorylation state. Our findings strongly suggest that there is crosstalk between STAT5 and STAT3 pathways that could modulate their ability to regulate gene expression. © 2007 Elsevier Inc. All rights reserved. Fil:Rodriguez Granillo, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Boffi, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v356_n3_p727_RodriguezGranillo http://hdl.handle.net/20.500.12110/paper_0006291X_v356_n3_p727_RodriguezGranillo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Gene expression HC11 Involution LIF Mammary epithelium Prolactin Signal transduction STAT3 STAT5 leukemia inhibitory factor prolactin STAT3 protein STAT5 protein article breast epithelium controlled study gene control gene expression regulation gene repression human human cell involution priority journal promoter region protein phosphorylation signal transduction transcription regulation Animals bcl-X Protein CCAAT-Enhancer-Binding Protein-delta Dexamethasone Epithelium Insulin Leukemia Inhibitory Factor Mammary Glands, Animal Mice Phosphorylation Prolactin Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos STAT3 Transcription Factor STAT5 Transcription Factor
spellingShingle	Gene expression HC11 Involution LIF Mammary epithelium Prolactin Signal transduction STAT3 STAT5 leukemia inhibitory factor prolactin STAT3 protein STAT5 protein article breast epithelium controlled study gene control gene expression regulation gene repression human human cell involution priority journal promoter region protein phosphorylation signal transduction transcription regulation Animals bcl-X Protein CCAAT-Enhancer-Binding Protein-delta Dexamethasone Epithelium Insulin Leukemia Inhibitory Factor Mammary Glands, Animal Mice Phosphorylation Prolactin Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos STAT3 Transcription Factor STAT5 Transcription Factor Rodríguez Granillo, Agustina Boffi, Juan Carlos Barañao, José Lino S. Kordon, Edith Claudia Pecci, Adali Guberman, Alejandra Sonia STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
topic_facet	Gene expression HC11 Involution LIF Mammary epithelium Prolactin Signal transduction STAT3 STAT5 leukemia inhibitory factor prolactin STAT3 protein STAT5 protein article breast epithelium controlled study gene control gene expression regulation gene repression human human cell involution priority journal promoter region protein phosphorylation signal transduction transcription regulation Animals bcl-X Protein CCAAT-Enhancer-Binding Protein-delta Dexamethasone Epithelium Insulin Leukemia Inhibitory Factor Mammary Glands, Animal Mice Phosphorylation Prolactin Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos STAT3 Transcription Factor STAT5 Transcription Factor
description	Gene regulation mediated by STAT factors has been implicated in cellular functions with relevance to a variety of processes. Particularly, STAT5 and STAT3 play a crucial role in mammary epithelium displaying reciprocal activation kinetics during pregnancy, lactation and involution. Here, we show that LIF treatment of mammary epithelial HC11 cells reduces the phosphorylation levels and transcriptional activity of p-STAT5 in correlation with STAT3 phosphorylation. We have also found that STAT5 activity is negatively modulated by this cytokine, both on a gene whose expression is induced, as well as on a promoter repressed by STAT5. Besides, our results show that lactogenic hormones increase LIF effect on gene induction without modifying STAT3 phosphorylation state. Our findings strongly suggest that there is crosstalk between STAT5 and STAT3 pathways that could modulate their ability to regulate gene expression. © 2007 Elsevier Inc. All rights reserved.
author	Rodríguez Granillo, Agustina Boffi, Juan Carlos Barañao, José Lino S. Kordon, Edith Claudia Pecci, Adali Guberman, Alejandra Sonia
author_facet	Rodríguez Granillo, Agustina Boffi, Juan Carlos Barañao, José Lino S. Kordon, Edith Claudia Pecci, Adali Guberman, Alejandra Sonia
author_sort	Rodríguez Granillo, Agustina
title	STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
title_short	STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
title_full	STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
title_fullStr	STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
title_full_unstemmed	STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
title_sort	stat5 transcriptional activity is impaired by lif in a mammary epithelial cell line
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v356_n3_p727_RodriguezGranillo http://hdl.handle.net/20.500.12110/paper_0006291X_v356_n3_p727_RodriguezGranillo
work_keys_str_mv	AT rodriguezgranilloagustina stat5transcriptionalactivityisimpairedbylifinamammaryepithelialcellline AT boffijuancarlos stat5transcriptionalactivityisimpairedbylifinamammaryepithelialcellline AT baranaojoselinos stat5transcriptionalactivityisimpairedbylifinamammaryepithelialcellline AT kordonedithclaudia stat5transcriptionalactivityisimpairedbylifinamammaryepithelialcellline AT pecciadali stat5transcriptionalactivityisimpairedbylifinamammaryepithelialcellline AT gubermanalejandrasonia stat5transcriptionalactivityisimpairedbylifinamammaryepithelialcellline
_version_	1768544666950565888

STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line

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