Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation
p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to mo...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v339_n1_p284_Malicet http://hdl.handle.net/20.500.12110/paper_0006291X_v339_n1_p284_Malicet |
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paper:paper_0006291X_v339_n1_p284_Malicet2023-06-08T14:30:15Z Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation Moreno de Colonna, Silvia Cell cycle Jab1 p27 p8 Two hybrid binding protein complementary DNA heat shock protein heat shock protein p 8 histidine jun activating binding 1 protein protein p27 unclassified drug article cell growth cellular distribution controlled study DNA library enzyme activity enzyme inhibition HeLa cell human human cell hybrid cell immunoprecipitation polyacrylamide gel electrophoresis priority journal protein degradation protein expression protein function protein induction protein localization protein protein interaction protein synthesis inhibition protein synthesis regulation p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to modulate their activity. We used two-hybrid screening of a pre-transformed human testes cDNA library to identify some of these partners. One of them was the multifunctional protein Jab1, its interaction with p8 being confirmed by His6-pull down and co-immunoprecipitation assays. In addition, we could show that the two proteins co-localized in the cell. Our functional data demonstrate that Jab1 requires direct interaction with p8 to induce the translocation of p27 from nucleus to cytoplasm and its subsequent degradation. Experiments showing that the knock-down of p8 expression results in a strong inhibition of Jab1 activity confirmed that the mechanism by which Jab1 promotes cell growth by decreasing p27 level is p8-dependent. © 2005 Elsevier Inc. All rights reserved. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v339_n1_p284_Malicet http://hdl.handle.net/20.500.12110/paper_0006291X_v339_n1_p284_Malicet |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell cycle Jab1 p27 p8 Two hybrid binding protein complementary DNA heat shock protein heat shock protein p 8 histidine jun activating binding 1 protein protein p27 unclassified drug article cell growth cellular distribution controlled study DNA library enzyme activity enzyme inhibition HeLa cell human human cell hybrid cell immunoprecipitation polyacrylamide gel electrophoresis priority journal protein degradation protein expression protein function protein induction protein localization protein protein interaction protein synthesis inhibition protein synthesis regulation |
| spellingShingle |
Cell cycle Jab1 p27 p8 Two hybrid binding protein complementary DNA heat shock protein heat shock protein p 8 histidine jun activating binding 1 protein protein p27 unclassified drug article cell growth cellular distribution controlled study DNA library enzyme activity enzyme inhibition HeLa cell human human cell hybrid cell immunoprecipitation polyacrylamide gel electrophoresis priority journal protein degradation protein expression protein function protein induction protein localization protein protein interaction protein synthesis inhibition protein synthesis regulation Moreno de Colonna, Silvia Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation |
| topic_facet |
Cell cycle Jab1 p27 p8 Two hybrid binding protein complementary DNA heat shock protein heat shock protein p 8 histidine jun activating binding 1 protein protein p27 unclassified drug article cell growth cellular distribution controlled study DNA library enzyme activity enzyme inhibition HeLa cell human human cell hybrid cell immunoprecipitation polyacrylamide gel electrophoresis priority journal protein degradation protein expression protein function protein induction protein localization protein protein interaction protein synthesis inhibition protein synthesis regulation |
| description |
p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to modulate their activity. We used two-hybrid screening of a pre-transformed human testes cDNA library to identify some of these partners. One of them was the multifunctional protein Jab1, its interaction with p8 being confirmed by His6-pull down and co-immunoprecipitation assays. In addition, we could show that the two proteins co-localized in the cell. Our functional data demonstrate that Jab1 requires direct interaction with p8 to induce the translocation of p27 from nucleus to cytoplasm and its subsequent degradation. Experiments showing that the knock-down of p8 expression results in a strong inhibition of Jab1 activity confirmed that the mechanism by which Jab1 promotes cell growth by decreasing p27 level is p8-dependent. © 2005 Elsevier Inc. All rights reserved. |
| author |
Moreno de Colonna, Silvia |
| author_facet |
Moreno de Colonna, Silvia |
| author_sort |
Moreno de Colonna, Silvia |
| title |
Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation |
| title_short |
Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation |
| title_full |
Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation |
| title_fullStr |
Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation |
| title_full_unstemmed |
Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation |
| title_sort |
interaction of the stress protein p8 with jab1 is required for jab1-dependent p27 nuclear-to-cytoplasm translocation |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v339_n1_p284_Malicet http://hdl.handle.net/20.500.12110/paper_0006291X_v339_n1_p284_Malicet |
| work_keys_str_mv |
AT morenodecolonnasilvia interactionofthestressproteinp8withjab1isrequiredforjab1dependentp27nucleartocytoplasmtranslocation |
| _version_ |
1768542671619489792 |