Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi

This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as...

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Publicado: 2001
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo
http://hdl.handle.net/20.500.12110/paper_0006291X_v281_n2_p300_Bollo
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spelling paper:paper_0006291X_v281_n2_p300_Bollo2023-06-08T14:30:12Z Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi Calcium Carbamylcholine Nicotine Nicotinic ligands Trypanosoma cruzi α-bungarotoxin alpha bungarotoxin carbachol cholinergic receptor nicotine article binding affinity calcium cell level calcium signaling epimastigote IC 50 ligand binding membrane binding nonhuman priority journal Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC50: 7.6×10-7M) and nicotine (IC50: 1×10-7M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite. © 2001 Academic Press. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo http://hdl.handle.net/20.500.12110/paper_0006291X_v281_n2_p300_Bollo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Calcium
Carbamylcholine
Nicotine
Nicotinic ligands
Trypanosoma cruzi
α-bungarotoxin
alpha bungarotoxin
carbachol
cholinergic receptor
nicotine
article
binding affinity
calcium cell level
calcium signaling
epimastigote
IC 50
ligand binding
membrane binding
nonhuman
priority journal
Trypanosoma cruzi
Protozoa
Trypanosoma
Trypanosoma cruzi
spellingShingle Calcium
Carbamylcholine
Nicotine
Nicotinic ligands
Trypanosoma cruzi
α-bungarotoxin
alpha bungarotoxin
carbachol
cholinergic receptor
nicotine
article
binding affinity
calcium cell level
calcium signaling
epimastigote
IC 50
ligand binding
membrane binding
nonhuman
priority journal
Trypanosoma cruzi
Protozoa
Trypanosoma
Trypanosoma cruzi
Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
topic_facet Calcium
Carbamylcholine
Nicotine
Nicotinic ligands
Trypanosoma cruzi
α-bungarotoxin
alpha bungarotoxin
carbachol
cholinergic receptor
nicotine
article
binding affinity
calcium cell level
calcium signaling
epimastigote
IC 50
ligand binding
membrane binding
nonhuman
priority journal
Trypanosoma cruzi
Protozoa
Trypanosoma
Trypanosoma cruzi
description This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC50: 7.6×10-7M) and nicotine (IC50: 1×10-7M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite. © 2001 Academic Press.
title Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
title_short Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
title_full Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
title_fullStr Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
title_full_unstemmed Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
title_sort binding of nicotinic ligands to and nicotine-induced calcium signaling in trypanosoma cruzi
publishDate 2001
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo
http://hdl.handle.net/20.500.12110/paper_0006291X_v281_n2_p300_Bollo
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