Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as...
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2001
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo http://hdl.handle.net/20.500.12110/paper_0006291X_v281_n2_p300_Bollo |
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paper:paper_0006291X_v281_n2_p300_Bollo2023-06-08T14:30:12Z Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi Calcium Carbamylcholine Nicotine Nicotinic ligands Trypanosoma cruzi α-bungarotoxin alpha bungarotoxin carbachol cholinergic receptor nicotine article binding affinity calcium cell level calcium signaling epimastigote IC 50 ligand binding membrane binding nonhuman priority journal Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC50: 7.6×10-7M) and nicotine (IC50: 1×10-7M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite. © 2001 Academic Press. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo http://hdl.handle.net/20.500.12110/paper_0006291X_v281_n2_p300_Bollo |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Calcium Carbamylcholine Nicotine Nicotinic ligands Trypanosoma cruzi α-bungarotoxin alpha bungarotoxin carbachol cholinergic receptor nicotine article binding affinity calcium cell level calcium signaling epimastigote IC 50 ligand binding membrane binding nonhuman priority journal Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
spellingShingle |
Calcium Carbamylcholine Nicotine Nicotinic ligands Trypanosoma cruzi α-bungarotoxin alpha bungarotoxin carbachol cholinergic receptor nicotine article binding affinity calcium cell level calcium signaling epimastigote IC 50 ligand binding membrane binding nonhuman priority journal Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
topic_facet |
Calcium Carbamylcholine Nicotine Nicotinic ligands Trypanosoma cruzi α-bungarotoxin alpha bungarotoxin carbachol cholinergic receptor nicotine article binding affinity calcium cell level calcium signaling epimastigote IC 50 ligand binding membrane binding nonhuman priority journal Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
description |
This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC50: 7.6×10-7M) and nicotine (IC50: 1×10-7M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite. © 2001 Academic Press. |
title |
Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
title_short |
Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
title_full |
Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
title_fullStr |
Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
title_full_unstemmed |
Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
title_sort |
binding of nicotinic ligands to and nicotine-induced calcium signaling in trypanosoma cruzi |
publishDate |
2001 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo http://hdl.handle.net/20.500.12110/paper_0006291X_v281_n2_p300_Bollo |
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1768544803050487808 |