Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol

Previous work indicated that diacylglycerol (DG) molecules translocate across the cytoplasm of mammalian cells, a process relevant to the signalling role of this lipid as protein kinase C activator. Here we investigated the possible mechanism underlying DG translocation. We examined the interaction...

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Autores principales:	Florin-Christensen, Jorge, D'Alessio, Cecilia, Arighi, Cecilia N., Jacobsen de Florín-Christensen, Mónica O.
Publicado:	1998
Materias:	diacylglycerol lipoprotein protein kinase C activator sepharose animal cell article binding affinity column chromatography cytoplasm intracellular transport lipid transport liver cytosol micelle nonhuman priority journal protein interaction rat Animalia Insecta Mammalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v243_n3_p669_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_0006291X_v243_n3_p669_FlorinChristensen
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v243_n3_p669_FlorinChristensen
record_format	dspace
spelling	paper:paper_0006291X_v243_n3_p669_FlorinChristensen2023-06-08T14:30:09Z Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol Florin-Christensen, Jorge D'Alessio, Cecilia Arighi, Cecilia N. Jacobsen de Florín-Christensen, Mónica O. diacylglycerol lipoprotein protein kinase C activator sepharose animal cell article binding affinity column chromatography cytoplasm intracellular transport lipid transport liver cytosol micelle nonhuman priority journal protein interaction rat Animalia Insecta Mammalia Previous work indicated that diacylglycerol (DG) molecules translocate across the cytoplasm of mammalian cells, a process relevant to the signalling role of this lipid as protein kinase C activator. Here we investigated the possible mechanism underlying DG translocation. We examined the interaction between 1,2-di-[1-14C]oleoyl-sn-glycerol and rat liver cytosol (rlc) using assays based on Lipidex-1000 and on coelution on Sepharose CL 6B. We measured high DG binding activity and found that it resides in cytosolic proteins and not in cytosolic lipids. Chromatography of rlc proteins on Sepharose CL 6B showed profiles in which the activity measured by either method coincided. Further, we showed that the DG-rlc protein interaction results in the stabilization of DG in a micellar form, eluting in the void volume of Sepharose CL 6B. Such stabilized micelles are reminiscent of insect lipophorins and may represent a new, thus far unrecognized, mode of lipid transport within living cells. Fil:Florin-Christensen, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:D'Alessio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Arighi, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Florin-Christensen, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v243_n3_p669_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_0006291X_v243_n3_p669_FlorinChristensen
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	diacylglycerol lipoprotein protein kinase C activator sepharose animal cell article binding affinity column chromatography cytoplasm intracellular transport lipid transport liver cytosol micelle nonhuman priority journal protein interaction rat Animalia Insecta Mammalia
spellingShingle	diacylglycerol lipoprotein protein kinase C activator sepharose animal cell article binding affinity column chromatography cytoplasm intracellular transport lipid transport liver cytosol micelle nonhuman priority journal protein interaction rat Animalia Insecta Mammalia Florin-Christensen, Jorge D'Alessio, Cecilia Arighi, Cecilia N. Jacobsen de Florín-Christensen, Mónica O. Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
topic_facet	diacylglycerol lipoprotein protein kinase C activator sepharose animal cell article binding affinity column chromatography cytoplasm intracellular transport lipid transport liver cytosol micelle nonhuman priority journal protein interaction rat Animalia Insecta Mammalia
description	Previous work indicated that diacylglycerol (DG) molecules translocate across the cytoplasm of mammalian cells, a process relevant to the signalling role of this lipid as protein kinase C activator. Here we investigated the possible mechanism underlying DG translocation. We examined the interaction between 1,2-di-[1-14C]oleoyl-sn-glycerol and rat liver cytosol (rlc) using assays based on Lipidex-1000 and on coelution on Sepharose CL 6B. We measured high DG binding activity and found that it resides in cytosolic proteins and not in cytosolic lipids. Chromatography of rlc proteins on Sepharose CL 6B showed profiles in which the activity measured by either method coincided. Further, we showed that the DG-rlc protein interaction results in the stabilization of DG in a micellar form, eluting in the void volume of Sepharose CL 6B. Such stabilized micelles are reminiscent of insect lipophorins and may represent a new, thus far unrecognized, mode of lipid transport within living cells.
author	Florin-Christensen, Jorge D'Alessio, Cecilia Arighi, Cecilia N. Jacobsen de Florín-Christensen, Mónica O.
author_facet	Florin-Christensen, Jorge D'Alessio, Cecilia Arighi, Cecilia N. Jacobsen de Florín-Christensen, Mónica O.
author_sort	Florin-Christensen, Jorge
title	Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
title_short	Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
title_full	Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
title_fullStr	Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
title_full_unstemmed	Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
title_sort	micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
publishDate	1998
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v243_n3_p669_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_0006291X_v243_n3_p669_FlorinChristensen
work_keys_str_mv	AT florinchristensenjorge micellarlipoproteinsasthepossiblestorageandtranslocationformofintracellulardiacylglycerol AT dalessiocecilia micellarlipoproteinsasthepossiblestorageandtranslocationformofintracellulardiacylglycerol AT arighicecilian micellarlipoproteinsasthepossiblestorageandtranslocationformofintracellulardiacylglycerol AT jacobsendeflorinchristensenmonicao micellarlipoproteinsasthepossiblestorageandtranslocationformofintracellulardiacylglycerol
_version_	1768542014331158528

Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol

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