Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids

Putrescine uptake in Trypanosoma cruzi epimastigotes is 10 to 50-fold higher than in Leishmania mexicana or Crithidia fasciculata. Polyamine transport in all these trypanosomatids is an energy-dependent process strongly inhibited by the presence of 2,4-dinitrophenol or KCN. Putrescine uptake in T. c...

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Autores principales:	González, Nélida Susana, Ceriani, María Carolina, Algranati, Israel David
Publicado:	1992
Materias:	2,4 dinitrophenol carbonyl cyanide chlorophenylhydrazone potassium cyanide putrescine amine transport article epimastigote leishmania mexicana nonhuman priority journal trypanosoma cruzi Animal Biological Transport Crithidia fasciculata Eflornithine Homeostasis Kinetics Leishmania mexicana Putrescine Support, Non-U.S. Gov't Temperature Trypanosoma cruzi Strophanthus gratus Trypanosoma Trypanosomatidae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v188_n1_p120_Gonzalez http://hdl.handle.net/20.500.12110/paper_0006291X_v188_n1_p120_Gonzalez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v188_n1_p120_Gonzalez
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spelling	paper:paper_0006291X_v188_n1_p120_Gonzalez2023-06-08T14:30:08Z Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids González, Nélida Susana Ceriani, María Carolina Algranati, Israel David 2,4 dinitrophenol carbonyl cyanide chlorophenylhydrazone potassium cyanide putrescine amine transport article epimastigote leishmania mexicana nonhuman priority journal trypanosoma cruzi Animal Biological Transport Crithidia fasciculata Eflornithine Homeostasis Kinetics Leishmania mexicana Putrescine Support, Non-U.S. Gov't Temperature Trypanosoma cruzi Crithidia fasciculata Leishmania mexicana Strophanthus gratus Trypanosoma Trypanosoma cruzi Trypanosomatidae Putrescine uptake in Trypanosoma cruzi epimastigotes is 10 to 50-fold higher than in Leishmania mexicana or Crithidia fasciculata. Polyamine transport in all these trypanosomatids is an energy-dependent process strongly inhibited by the presence of 2,4-dinitrophenol or KCN. Putrescine uptake in T. cruzi and L. mexicana was markedly decreased by the proton ionophore carbonylcyanide m-chlorophenylhydrazone but it was not affected by ouabain, a Na+-K+ pump inhibitor. The depletion of intracellular polyamines by treatment of parasite cultures with α-difluoromethylornithine elicited a marked induction of putrescine uptake in L. mexicana and C. fasciculata by increasing considerably the Vmax of this process. Conversely, the uptake of putrescine in T. cruzi was essentially unchanged by the same treatment. The differential regulation of putrescine transport in T. cruzi might be related to some distinctive features of polyamine metabolism in this parasite. © 1992. Fil:Gonzalez, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceriani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v188_n1_p120_Gonzalez http://hdl.handle.net/20.500.12110/paper_0006291X_v188_n1_p120_Gonzalez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2,4 dinitrophenol carbonyl cyanide chlorophenylhydrazone potassium cyanide putrescine amine transport article epimastigote leishmania mexicana nonhuman priority journal trypanosoma cruzi Animal Biological Transport Crithidia fasciculata Eflornithine Homeostasis Kinetics Leishmania mexicana Putrescine Support, Non-U.S. Gov't Temperature Trypanosoma cruzi Crithidia fasciculata Leishmania mexicana Strophanthus gratus Trypanosoma Trypanosoma cruzi Trypanosomatidae
spellingShingle	2,4 dinitrophenol carbonyl cyanide chlorophenylhydrazone potassium cyanide putrescine amine transport article epimastigote leishmania mexicana nonhuman priority journal trypanosoma cruzi Animal Biological Transport Crithidia fasciculata Eflornithine Homeostasis Kinetics Leishmania mexicana Putrescine Support, Non-U.S. Gov't Temperature Trypanosoma cruzi Crithidia fasciculata Leishmania mexicana Strophanthus gratus Trypanosoma Trypanosoma cruzi Trypanosomatidae González, Nélida Susana Ceriani, María Carolina Algranati, Israel David Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
topic_facet	2,4 dinitrophenol carbonyl cyanide chlorophenylhydrazone potassium cyanide putrescine amine transport article epimastigote leishmania mexicana nonhuman priority journal trypanosoma cruzi Animal Biological Transport Crithidia fasciculata Eflornithine Homeostasis Kinetics Leishmania mexicana Putrescine Support, Non-U.S. Gov't Temperature Trypanosoma cruzi Crithidia fasciculata Leishmania mexicana Strophanthus gratus Trypanosoma Trypanosoma cruzi Trypanosomatidae
description	Putrescine uptake in Trypanosoma cruzi epimastigotes is 10 to 50-fold higher than in Leishmania mexicana or Crithidia fasciculata. Polyamine transport in all these trypanosomatids is an energy-dependent process strongly inhibited by the presence of 2,4-dinitrophenol or KCN. Putrescine uptake in T. cruzi and L. mexicana was markedly decreased by the proton ionophore carbonylcyanide m-chlorophenylhydrazone but it was not affected by ouabain, a Na+-K+ pump inhibitor. The depletion of intracellular polyamines by treatment of parasite cultures with α-difluoromethylornithine elicited a marked induction of putrescine uptake in L. mexicana and C. fasciculata by increasing considerably the Vmax of this process. Conversely, the uptake of putrescine in T. cruzi was essentially unchanged by the same treatment. The differential regulation of putrescine transport in T. cruzi might be related to some distinctive features of polyamine metabolism in this parasite. © 1992.
author	González, Nélida Susana Ceriani, María Carolina Algranati, Israel David
author_facet	González, Nélida Susana Ceriani, María Carolina Algranati, Israel David
author_sort	González, Nélida Susana
title	Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
title_short	Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
title_full	Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
title_fullStr	Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
title_full_unstemmed	Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
title_sort	differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids
publishDate	1992
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v188_n1_p120_Gonzalez http://hdl.handle.net/20.500.12110/paper_0006291X_v188_n1_p120_Gonzalez
work_keys_str_mv	AT gonzaleznelidasusana differentialregulationofputrescineuptakeintrypanosomacruziandothertrypanosomatids AT cerianimariacarolina differentialregulationofputrescineuptakeintrypanosomacruziandothertrypanosomatids AT algranatiisraeldavid differentialregulationofputrescineuptakeintrypanosomacruziandothertrypanosomatids
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Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids

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