In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata
Cuticle proteins of an insect pest, the Medfly Ceratitis capitata, were resolved in polyacrylamide gels and partially characterized. The pupal cuticle was found to be different from cuticles of other insects since more than 80% w w of the protein is a single mannose-containing polypeptide (PCG-100)....
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1989
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v164_n1_p251_Boccaccio http://hdl.handle.net/20.500.12110/paper_0006291X_v164_n1_p251_Boccaccio |
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paper:paper_0006291X_v164_n1_p251_Boccaccio2023-06-08T14:30:06Z In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata animal cell arthropod ceratitis capitata cuticle protein glycoprotein synthesis insect cuticle metamorphosis nonhuman priority journal Animal Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Glycoproteins Insects Metamorphosis, Biological Proteins Support, Non-U.S. Gov't Cuticle proteins of an insect pest, the Medfly Ceratitis capitata, were resolved in polyacrylamide gels and partially characterized. The pupal cuticle was found to be different from cuticles of other insects since more than 80% w w of the protein is a single mannose-containing polypeptide (PCG-100). The temporally-regulated in vivo biosynthesis and deposition of cuticle proteins was studied by microinjection of [35S]methionine followed by hand dissection of pupal cuticles. The major pupal glycoprotein, PCG-100, is cuticle- and stage-specific and was the earliest to be labeled and deposited. Its synthesis was maximal at around 46 hours after pupariation and then it decreased. The deposited PCG-100 and other minor pupal cuticle proteins become non-extractable at the end of the instar (7 days after pupariation) probably by sclerotization phenomena. These results provide insight into the temporal control of gene expression programs involved in cuticle deposition during medfly metamorphosis. © 1989. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v164_n1_p251_Boccaccio http://hdl.handle.net/20.500.12110/paper_0006291X_v164_n1_p251_Boccaccio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
animal cell arthropod ceratitis capitata cuticle protein glycoprotein synthesis insect cuticle metamorphosis nonhuman priority journal Animal Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Glycoproteins Insects Metamorphosis, Biological Proteins Support, Non-U.S. Gov't |
| spellingShingle |
animal cell arthropod ceratitis capitata cuticle protein glycoprotein synthesis insect cuticle metamorphosis nonhuman priority journal Animal Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Glycoproteins Insects Metamorphosis, Biological Proteins Support, Non-U.S. Gov't In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata |
| topic_facet |
animal cell arthropod ceratitis capitata cuticle protein glycoprotein synthesis insect cuticle metamorphosis nonhuman priority journal Animal Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Glycoproteins Insects Metamorphosis, Biological Proteins Support, Non-U.S. Gov't |
| description |
Cuticle proteins of an insect pest, the Medfly Ceratitis capitata, were resolved in polyacrylamide gels and partially characterized. The pupal cuticle was found to be different from cuticles of other insects since more than 80% w w of the protein is a single mannose-containing polypeptide (PCG-100). The temporally-regulated in vivo biosynthesis and deposition of cuticle proteins was studied by microinjection of [35S]methionine followed by hand dissection of pupal cuticles. The major pupal glycoprotein, PCG-100, is cuticle- and stage-specific and was the earliest to be labeled and deposited. Its synthesis was maximal at around 46 hours after pupariation and then it decreased. The deposited PCG-100 and other minor pupal cuticle proteins become non-extractable at the end of the instar (7 days after pupariation) probably by sclerotization phenomena. These results provide insight into the temporal control of gene expression programs involved in cuticle deposition during medfly metamorphosis. © 1989. |
| title |
In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata |
| title_short |
In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata |
| title_full |
In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata |
| title_fullStr |
In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata |
| title_full_unstemmed |
In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata |
| title_sort |
in vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly ceratitis capitata |
| publishDate |
1989 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v164_n1_p251_Boccaccio http://hdl.handle.net/20.500.12110/paper_0006291X_v164_n1_p251_Boccaccio |
| _version_ |
1768544666772307968 |