Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid

Xanthan gum is a complex polysaccharide produced by Xanthomonas campestris, composed of pentasaccharide repeating units containing mannose, glucose and glucuronic acid (in the ratio 2:2:1) and pyruvic acid acetal and 0-acetyl residues in differing proportions Jansson et al. (1975) Carbohyd.Res. 45,...

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Detalles Bibliográficos
Autores principales:	Ielpi, Luis, Couso, Roberto O., Dankert, Marcelo Alberto
Publicado:	1981
Materias:	bacterial polysaccharide glycolipid phosphoenolpyruvate pyruvic acid pyruvic acid derivative xanthan article biosynthesis ion exchange chromatography metabolism paper chromatography Xanthomonas Chromatography, DEAE-Cellulose Chromatography, Paper Glycolipids Phosphoenolpyruvate Polysaccharides, Bacterial Pyruvates Pyruvic Acid
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v102_n4_p1400_Ielpi http://hdl.handle.net/20.500.12110/paper_0006291X_v102_n4_p1400_Ielpi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v102_n4_p1400_Ielpi
record_format	dspace
spelling	paper:paper_0006291X_v102_n4_p1400_Ielpi2023-06-08T14:30:00Z Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid Ielpi, Luis Couso, Roberto O. Dankert, Marcelo Alberto bacterial polysaccharide glycolipid phosphoenolpyruvate pyruvic acid pyruvic acid derivative xanthan article biosynthesis ion exchange chromatography metabolism paper chromatography Xanthomonas Chromatography, DEAE-Cellulose Chromatography, Paper Glycolipids Phosphoenolpyruvate Polysaccharides, Bacterial Pyruvates Pyruvic Acid Xanthomonas Xanthan gum is a complex polysaccharide produced by Xanthomonas campestris, composed of pentasaccharide repeating units containing mannose, glucose and glucuronic acid (in the ratio 2:2:1) and pyruvic acid acetal and 0-acetyl residues in differing proportions Jansson et al. (1975) Carbohyd.Res. 45, 275-282). Previous work (Ielpi et al. FEBS Lett., in press) has shown that the pentasaccharide is first assembled on a pyrophosphate lipid and then polymerized to xanthan gum. It is now shown, using EDTA-treated cells and different combinations of the adequate [14C]labeled donors, that phosphoenol pyruvate provides the acetal residues and that the transfer occurs on the terminal mannose of the pentasaccharide-P-P-lipid, as judged by the solubility properties and DEAE-cellulose column chromatography of the compounds formed as well as by the behaviour of the substances liberated by mild acid and alkaline treatments and Smith degradation. The subsequent polymerization process leads to pyruvylated xanthan gum. © 1981 Academic Press, Inc. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couso, R.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v102_n4_p1400_Ielpi http://hdl.handle.net/20.500.12110/paper_0006291X_v102_n4_p1400_Ielpi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial polysaccharide glycolipid phosphoenolpyruvate pyruvic acid pyruvic acid derivative xanthan article biosynthesis ion exchange chromatography metabolism paper chromatography Xanthomonas Chromatography, DEAE-Cellulose Chromatography, Paper Glycolipids Phosphoenolpyruvate Polysaccharides, Bacterial Pyruvates Pyruvic Acid Xanthomonas
spellingShingle	bacterial polysaccharide glycolipid phosphoenolpyruvate pyruvic acid pyruvic acid derivative xanthan article biosynthesis ion exchange chromatography metabolism paper chromatography Xanthomonas Chromatography, DEAE-Cellulose Chromatography, Paper Glycolipids Phosphoenolpyruvate Polysaccharides, Bacterial Pyruvates Pyruvic Acid Xanthomonas Ielpi, Luis Couso, Roberto O. Dankert, Marcelo Alberto Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid
topic_facet	bacterial polysaccharide glycolipid phosphoenolpyruvate pyruvic acid pyruvic acid derivative xanthan article biosynthesis ion exchange chromatography metabolism paper chromatography Xanthomonas Chromatography, DEAE-Cellulose Chromatography, Paper Glycolipids Phosphoenolpyruvate Polysaccharides, Bacterial Pyruvates Pyruvic Acid Xanthomonas
description	Xanthan gum is a complex polysaccharide produced by Xanthomonas campestris, composed of pentasaccharide repeating units containing mannose, glucose and glucuronic acid (in the ratio 2:2:1) and pyruvic acid acetal and 0-acetyl residues in differing proportions Jansson et al. (1975) Carbohyd.Res. 45, 275-282). Previous work (Ielpi et al. FEBS Lett., in press) has shown that the pentasaccharide is first assembled on a pyrophosphate lipid and then polymerized to xanthan gum. It is now shown, using EDTA-treated cells and different combinations of the adequate [14C]labeled donors, that phosphoenol pyruvate provides the acetal residues and that the transfer occurs on the terminal mannose of the pentasaccharide-P-P-lipid, as judged by the solubility properties and DEAE-cellulose column chromatography of the compounds formed as well as by the behaviour of the substances liberated by mild acid and alkaline treatments and Smith degradation. The subsequent polymerization process leads to pyruvylated xanthan gum. © 1981 Academic Press, Inc.
author	Ielpi, Luis Couso, Roberto O. Dankert, Marcelo Alberto
author_facet	Ielpi, Luis Couso, Roberto O. Dankert, Marcelo Alberto
author_sort	Ielpi, Luis
title	Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid
title_short	Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid
title_full	Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid
title_fullStr	Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid
title_full_unstemmed	Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid
title_sort	xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-p-p-lipid
publishDate	1981
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v102_n4_p1400_Ielpi http://hdl.handle.net/20.500.12110/paper_0006291X_v102_n4_p1400_Ielpi
work_keys_str_mv	AT ielpiluis xanthancumbiosynthesispyruvicacidacetalresiduesaretransferredfromphosphoenolpyruvatetothepentasaccharidepplipid AT cousorobertoo xanthancumbiosynthesispyruvicacidacetalresiduesaretransferredfromphosphoenolpyruvatetothepentasaccharidepplipid AT dankertmarceloalberto xanthancumbiosynthesispyruvicacidacetalresiduesaretransferredfromphosphoenolpyruvatetothepentasaccharidepplipid
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Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid

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