Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms
In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high perf...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v1300_n3_p233_Uhrig http://hdl.handle.net/20.500.12110/paper_00052760_v1300_n3_p233_Uhrig |
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paper:paper_00052760_v1300_n3_p233_Uhrig2023-06-08T14:29:56Z Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms Inositolphospholipid T. cruzi Trypomastigote inositol derivative phospholipid ceramide diacylglycerol fatty acid glycerylphosphoinositol glycosylphosphatidylinositol inositol phosphate palmitic acid palmitic acid derivative phosphatidylinositol sphingosine article high performance liquid chromatography ion exchange chromatography isotope labeling life cycle lipid analysis priority journal thin layer chromatography Trypanosoma cruzi animal chemical structure chemistry isolation and purification metabolism Trypanosoma Trypanosoma cruzi Animals Ceramides Chromatography, High Pressure Liquid Chromatography, Thin Layer Diglycerides Fatty Acids Glycosylphosphatidylinositols Inositol Phosphates Molecular Structure Palmitic Acid Palmitic Acids Phosphatidylinositols Sphingosine Trypanosoma cruzi In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high performance liquid chromatography and thin layer chromatography. Specific phosphatidyl-inositol phospholipase C digestion, dephosphorylation and acid methanolysis showed a ceramide structure for the lower migrating IPL1. Palmitoyldihydrosphingosine and palmitoylsphingosine were detected by reverse-phase thin-layer chromatography. On the other hand, IPL2 showed to be a mixture of diacylglycero- and alkylacylglycero-phospholipids in a 1:1 ratio. After PI-PLC digestion, the lipids were separated by preparative TLC and individually analysed. The diacylglycerol contained mainly C18:0 fatty acid together with a low amount of C16:0. Hexadecylglycerol esterified with the C18:0 fatty acid was the only alkylacylglycerol detected. The C18:2 and C18:1 fatty acids, preponderant in the PI molecules of epimastigote forms, were not detected in trypomastigote forms. This is the first report on inositol phospholipids, putative precursors of lipid anchors in the infective stage of T. cruzi. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v1300_n3_p233_Uhrig http://hdl.handle.net/20.500.12110/paper_00052760_v1300_n3_p233_Uhrig |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Inositolphospholipid T. cruzi Trypomastigote inositol derivative phospholipid ceramide diacylglycerol fatty acid glycerylphosphoinositol glycosylphosphatidylinositol inositol phosphate palmitic acid palmitic acid derivative phosphatidylinositol sphingosine article high performance liquid chromatography ion exchange chromatography isotope labeling life cycle lipid analysis priority journal thin layer chromatography Trypanosoma cruzi animal chemical structure chemistry isolation and purification metabolism Trypanosoma Trypanosoma cruzi Animals Ceramides Chromatography, High Pressure Liquid Chromatography, Thin Layer Diglycerides Fatty Acids Glycosylphosphatidylinositols Inositol Phosphates Molecular Structure Palmitic Acid Palmitic Acids Phosphatidylinositols Sphingosine Trypanosoma cruzi |
| spellingShingle |
Inositolphospholipid T. cruzi Trypomastigote inositol derivative phospholipid ceramide diacylglycerol fatty acid glycerylphosphoinositol glycosylphosphatidylinositol inositol phosphate palmitic acid palmitic acid derivative phosphatidylinositol sphingosine article high performance liquid chromatography ion exchange chromatography isotope labeling life cycle lipid analysis priority journal thin layer chromatography Trypanosoma cruzi animal chemical structure chemistry isolation and purification metabolism Trypanosoma Trypanosoma cruzi Animals Ceramides Chromatography, High Pressure Liquid Chromatography, Thin Layer Diglycerides Fatty Acids Glycosylphosphatidylinositols Inositol Phosphates Molecular Structure Palmitic Acid Palmitic Acids Phosphatidylinositols Sphingosine Trypanosoma cruzi Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| topic_facet |
Inositolphospholipid T. cruzi Trypomastigote inositol derivative phospholipid ceramide diacylglycerol fatty acid glycerylphosphoinositol glycosylphosphatidylinositol inositol phosphate palmitic acid palmitic acid derivative phosphatidylinositol sphingosine article high performance liquid chromatography ion exchange chromatography isotope labeling life cycle lipid analysis priority journal thin layer chromatography Trypanosoma cruzi animal chemical structure chemistry isolation and purification metabolism Trypanosoma Trypanosoma cruzi Animals Ceramides Chromatography, High Pressure Liquid Chromatography, Thin Layer Diglycerides Fatty Acids Glycosylphosphatidylinositols Inositol Phosphates Molecular Structure Palmitic Acid Palmitic Acids Phosphatidylinositols Sphingosine Trypanosoma cruzi |
| description |
In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high performance liquid chromatography and thin layer chromatography. Specific phosphatidyl-inositol phospholipase C digestion, dephosphorylation and acid methanolysis showed a ceramide structure for the lower migrating IPL1. Palmitoyldihydrosphingosine and palmitoylsphingosine were detected by reverse-phase thin-layer chromatography. On the other hand, IPL2 showed to be a mixture of diacylglycero- and alkylacylglycero-phospholipids in a 1:1 ratio. After PI-PLC digestion, the lipids were separated by preparative TLC and individually analysed. The diacylglycerol contained mainly C18:0 fatty acid together with a low amount of C16:0. Hexadecylglycerol esterified with the C18:0 fatty acid was the only alkylacylglycerol detected. The C18:2 and C18:1 fatty acids, preponderant in the PI molecules of epimastigote forms, were not detected in trypomastigote forms. This is the first report on inositol phospholipids, putative precursors of lipid anchors in the infective stage of T. cruzi. |
| title |
Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| title_short |
Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| title_full |
Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| title_fullStr |
Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| title_full_unstemmed |
Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms |
| title_sort |
characterization of inositolphospholipids in tryparzosoma cruzi trypmastigote forms |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v1300_n3_p233_Uhrig http://hdl.handle.net/20.500.12110/paper_00052760_v1300_n3_p233_Uhrig |
| _version_ |
1768544530314821632 |