Fructokinase from rat liver. I. Purification and properties

Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearl...

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Autores principales:	González, Nélida Susana, Pontis Videla, Horacio G.
Publicado:	1971
Materias:	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez http://hdl.handle.net/20.500.12110/paper_00052744_v227_n1_p67_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00052744_v227_n1_p67_Sanchez
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spelling	paper:paper_00052744_v227_n1_p67_Sanchez2023-06-08T14:29:55Z Fructokinase from rat liver. I. Purification and properties González, Nélida Susana Pontis Videla, Horacio G. 4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearly homogenous by density gradient centrifugation but gives a single peak in sedimentation velocity analysis. Purified liver fructokinase has a Km of 0.46-0.80 mM for fructose and 1.56-1.33 mM for MgATP at a K+ concentration of 0.4 and 0.1 M, respectively. The enzyme also phosphorylates l-sorbose and d-tagatose. No difference could be found in the phosphorylation of the pyranose and furanose forms of fructose. The enzyme is inhibited by p-chloromercuribenzoate and is stable up to 50-55°. © 1971. Fil:González, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pontis, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1971 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez http://hdl.handle.net/20.500.12110/paper_00052744_v227_n1_p67_Sanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry
spellingShingle	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry González, Nélida Susana Pontis Videla, Horacio G. Fructokinase from rat liver. I. Purification and properties
topic_facet	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry
description	Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearly homogenous by density gradient centrifugation but gives a single peak in sedimentation velocity analysis. Purified liver fructokinase has a Km of 0.46-0.80 mM for fructose and 1.56-1.33 mM for MgATP at a K+ concentration of 0.4 and 0.1 M, respectively. The enzyme also phosphorylates l-sorbose and d-tagatose. No difference could be found in the phosphorylation of the pyranose and furanose forms of fructose. The enzyme is inhibited by p-chloromercuribenzoate and is stable up to 50-55°. © 1971.
author	González, Nélida Susana Pontis Videla, Horacio G.
author_facet	González, Nélida Susana Pontis Videla, Horacio G.
author_sort	González, Nélida Susana
title	Fructokinase from rat liver. I. Purification and properties
title_short	Fructokinase from rat liver. I. Purification and properties
title_full	Fructokinase from rat liver. I. Purification and properties
title_fullStr	Fructokinase from rat liver. I. Purification and properties
title_full_unstemmed	Fructokinase from rat liver. I. Purification and properties
title_sort	fructokinase from rat liver. i. purification and properties
publishDate	1971
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez http://hdl.handle.net/20.500.12110/paper_00052744_v227_n1_p67_Sanchez
work_keys_str_mv	AT gonzaleznelidasusana fructokinasefromratliveripurificationandproperties AT pontisvidelahoraciog fructokinasefromratliveripurificationandproperties
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Fructokinase from rat liver. I. Purification and properties

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