Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose

A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species foun...

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Autor principal:	Schebor, Carolina C.
Publicado:	2005
Materias:	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Freeze Drying Phosphatidylethanolamines Trehalose Mammalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1713_n1_p57_Ohtake http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00052736_v1713_n1_p57_Ohtake
record_format	dspace
spelling	paper:paper_00052736_v1713_n1_p57_Ohtake2023-06-08T14:29:49Z Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose Schebor, Carolina C. Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved. Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1713_n1_p57_Ohtake http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia
spellingShingle	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia Schebor, Carolina C. Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
topic_facet	Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cholesterol Freeze Drying Phosphatidylethanolamines Trehalose Mammalia
description	A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.
author	Schebor, Carolina C.
author_facet	Schebor, Carolina C.
author_sort	Schebor, Carolina C.
title	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_short	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_full	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_fullStr	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_full_unstemmed	Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_sort	phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1713_n1_p57_Ohtake http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
work_keys_str_mv	AT scheborcarolinac phasebehavioroffreezedriedphospholipidcholesterolmixturesstabilizedwithtrehalose
_version_	1768546566371540992

Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose

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