Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not fo...
Guardado en:
Autores principales: | , |
---|---|
Publicado: |
1977
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p334_Flawia http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia |
Aporte de: |
id |
paper:paper_00039861_v180_n2_p334_Flawia |
---|---|
record_format |
dspace |
spelling |
paper:paper_00039861_v180_n2_p334_Flawia2023-06-08T14:24:57Z Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity Flawiá, Mirtha María Torres, Héctor Norberto adenylate cyclase enzyme deficiency in vitro study microorganism mutant neurospora crassa theoretical study Adenylate Cyclase Crosses, Genetic Membranes Mutation Neurospora Neurospora crassa Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the "crisp" morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. © 1977. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p334_Flawia http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
adenylate cyclase enzyme deficiency in vitro study microorganism mutant neurospora crassa theoretical study Adenylate Cyclase Crosses, Genetic Membranes Mutation Neurospora Neurospora crassa |
spellingShingle |
adenylate cyclase enzyme deficiency in vitro study microorganism mutant neurospora crassa theoretical study Adenylate Cyclase Crosses, Genetic Membranes Mutation Neurospora Neurospora crassa Flawiá, Mirtha María Torres, Héctor Norberto Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
topic_facet |
adenylate cyclase enzyme deficiency in vitro study microorganism mutant neurospora crassa theoretical study Adenylate Cyclase Crosses, Genetic Membranes Mutation Neurospora Neurospora crassa |
description |
Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the "crisp" morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. © 1977. |
author |
Flawiá, Mirtha María Torres, Héctor Norberto |
author_facet |
Flawiá, Mirtha María Torres, Héctor Norberto |
author_sort |
Flawiá, Mirtha María |
title |
Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
title_short |
Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
title_full |
Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
title_fullStr |
Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
title_full_unstemmed |
Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity |
title_sort |
characterization of neurospora crassa mutant strains deficient in adenylate cyclase activity |
publishDate |
1977 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p334_Flawia http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia |
work_keys_str_mv |
AT flawiamirthamaria characterizationofneurosporacrassamutantstrainsdeficientinadenylatecyclaseactivity AT torreshectornorberto characterizationofneurosporacrassamutantstrainsdeficientinadenylatecyclaseactivity |
_version_ |
1768543111309426688 |