Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes

Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear...

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Autores principales: Cantore de Ezcurra, María Leonor, Recondo, Eduardo Francisco
Publicado: 1977
Materias:
carbohydrate
guanine
hypoxanthine
nucleotide
radioisotope
choline c 14
development
egg
in vitro study
theoretical study
Animal
Bufo arenarum
Choline
Cytidine Diphosphate Choline
Female
Nucleoside Diphosphate Sugars
Nucleotides
Oocytes
Oogenesis
Ovum
Pentoses
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p269_Cantore
http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p269_Cantore
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00039861_v180_n2_p269_Cantore
record_format dspace
spelling paper:paper_00039861_v180_n2_p269_Cantore2023-06-08T14:24:57Z Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes Cantore de Ezcurra, María Leonor Recondo, Eduardo Francisco carbohydrate guanine hypoxanthine nucleotide radioisotope choline c 14 development egg in vitro study theoretical study Animal Bufo arenarum Choline Cytidine Diphosphate Choline Female Nucleoside Diphosphate Sugars Nucleotides Oocytes Oogenesis Ovum Pentoses Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear gradient of ammonium chloride. The first peak of ultraviolet adsorption eluted from the resin was a complex mixture of at least three substances. The main component was identified as cytidine diphosphocholine by chemical, enzymatic, and chromatographic analyses. Preliminary experiments suggest a possible role for this compound during oogenesis, since immature oocytes incubated in vitro with [14C]choline showed an active metabolism of this substance with rapid incorporation in choline phosphate, cytidine diphosphocholine, and lecithin. © 1977. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Recondo, E.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p269_Cantore http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p269_Cantore
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic carbohydrate
guanine
hypoxanthine
nucleotide
radioisotope
choline c 14
development
egg
in vitro study
theoretical study
Animal
Bufo arenarum
Choline
Cytidine Diphosphate Choline
Female
Nucleoside Diphosphate Sugars
Nucleotides
Oocytes
Oogenesis
Ovum
Pentoses
spellingShingle carbohydrate
guanine
hypoxanthine
nucleotide
radioisotope
choline c 14
development
egg
in vitro study
theoretical study
Animal
Bufo arenarum
Choline
Cytidine Diphosphate Choline
Female
Nucleoside Diphosphate Sugars
Nucleotides
Oocytes
Oogenesis
Ovum
Pentoses
Cantore de Ezcurra, María Leonor
Recondo, Eduardo Francisco
Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
topic_facet carbohydrate
guanine
hypoxanthine
nucleotide
radioisotope
choline c 14
development
egg
in vitro study
theoretical study
Animal
Bufo arenarum
Choline
Cytidine Diphosphate Choline
Female
Nucleoside Diphosphate Sugars
Nucleotides
Oocytes
Oogenesis
Ovum
Pentoses
description Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear gradient of ammonium chloride. The first peak of ultraviolet adsorption eluted from the resin was a complex mixture of at least three substances. The main component was identified as cytidine diphosphocholine by chemical, enzymatic, and chromatographic analyses. Preliminary experiments suggest a possible role for this compound during oogenesis, since immature oocytes incubated in vitro with [14C]choline showed an active metabolism of this substance with rapid incorporation in choline phosphate, cytidine diphosphocholine, and lecithin. © 1977.
author Cantore de Ezcurra, María Leonor
Recondo, Eduardo Francisco
author_facet Cantore de Ezcurra, María Leonor
Recondo, Eduardo Francisco
author_sort Cantore de Ezcurra, María Leonor
title Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
title_short Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
title_full Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
title_fullStr Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
title_full_unstemmed Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
title_sort nucleotides and sugar nucleotides in early development of bufo arenarum. coelomic oocytes
publishDate 1977
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p269_Cantore
http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p269_Cantore
work_keys_str_mv AT cantoredeezcurramarialeonor nucleotidesandsugarnucleotidesinearlydevelopmentofbufoarenarumcoelomicoocytes
AT recondoeduardofrancisco nucleotidesandsugarnucleotidesinearlydevelopmentofbufoarenarumcoelomicoocytes
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