In vitro synthesis of particulate glycogen from uridine diphosphate glucose

High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose do...

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Detalles Bibliográficos
Autores principales: Parodi, Armando José, Krisman de Fischman, Clara Rebeca
Publicado: 1969
Materias:
glucose
glucosyltransferase
glycogen
hydrochloric acid
iodine
pyrimidine nucleotide
sodium hydroxide
animal
article
biosynthesis
density gradient centrifugation
enzymology
liver
metabolism
molecular weight
pH
rat
spectroscopy
Animal
Centrifugation, Density Gradient
Glucose
Glucosyltransferases
Glycogen
Hydrochloric Acid
Hydrogen-Ion Concentration
Iodine
Liver
Molecular Weight
Rats
Sodium Hydroxide
Spectrum Analysis
Uracil Nucleotides
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v132_n1_p111_Parodi
http://hdl.handle.net/20.500.12110/paper_00039861_v132_n1_p111_Parodi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor. The product obtained did not differ significantly from the native glycogen as judged by iodine spectrum, sedimentation coefficient in sucrose gradients, and by the effect of treatment with acid or alkali. Glycogen obtained from uridine diphosphate glucose differed from that prepared with glucose 1-phosphate as glucosyl donor. © 1969.

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