In vitro synthesis of particulate glycogen from uridine diphosphate glucose
High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose do...
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1969
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v132_n1_p111_Parodi http://hdl.handle.net/20.500.12110/paper_00039861_v132_n1_p111_Parodi |
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paper:paper_00039861_v132_n1_p111_Parodi2023-06-08T14:24:54Z In vitro synthesis of particulate glycogen from uridine diphosphate glucose Parodi, Armando José Krisman de Fischman, Clara Rebeca glucose glucosyltransferase glycogen hydrochloric acid iodine pyrimidine nucleotide sodium hydroxide animal article biosynthesis density gradient centrifugation enzymology liver metabolism molecular weight pH rat spectroscopy Animal Centrifugation, Density Gradient Glucose Glucosyltransferases Glycogen Hydrochloric Acid Hydrogen-Ion Concentration Iodine Liver Molecular Weight Rats Sodium Hydroxide Spectrum Analysis Uracil Nucleotides High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor. The product obtained did not differ significantly from the native glycogen as judged by iodine spectrum, sedimentation coefficient in sucrose gradients, and by the effect of treatment with acid or alkali. Glycogen obtained from uridine diphosphate glucose differed from that prepared with glucose 1-phosphate as glucosyl donor. © 1969. Fil:Parodi, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1969 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v132_n1_p111_Parodi http://hdl.handle.net/20.500.12110/paper_00039861_v132_n1_p111_Parodi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
glucose glucosyltransferase glycogen hydrochloric acid iodine pyrimidine nucleotide sodium hydroxide animal article biosynthesis density gradient centrifugation enzymology liver metabolism molecular weight pH rat spectroscopy Animal Centrifugation, Density Gradient Glucose Glucosyltransferases Glycogen Hydrochloric Acid Hydrogen-Ion Concentration Iodine Liver Molecular Weight Rats Sodium Hydroxide Spectrum Analysis Uracil Nucleotides |
| spellingShingle |
glucose glucosyltransferase glycogen hydrochloric acid iodine pyrimidine nucleotide sodium hydroxide animal article biosynthesis density gradient centrifugation enzymology liver metabolism molecular weight pH rat spectroscopy Animal Centrifugation, Density Gradient Glucose Glucosyltransferases Glycogen Hydrochloric Acid Hydrogen-Ion Concentration Iodine Liver Molecular Weight Rats Sodium Hydroxide Spectrum Analysis Uracil Nucleotides Parodi, Armando José Krisman de Fischman, Clara Rebeca In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| topic_facet |
glucose glucosyltransferase glycogen hydrochloric acid iodine pyrimidine nucleotide sodium hydroxide animal article biosynthesis density gradient centrifugation enzymology liver metabolism molecular weight pH rat spectroscopy Animal Centrifugation, Density Gradient Glucose Glucosyltransferases Glycogen Hydrochloric Acid Hydrogen-Ion Concentration Iodine Liver Molecular Weight Rats Sodium Hydroxide Spectrum Analysis Uracil Nucleotides |
| description |
High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor. The product obtained did not differ significantly from the native glycogen as judged by iodine spectrum, sedimentation coefficient in sucrose gradients, and by the effect of treatment with acid or alkali. Glycogen obtained from uridine diphosphate glucose differed from that prepared with glucose 1-phosphate as glucosyl donor. © 1969. |
| author |
Parodi, Armando José Krisman de Fischman, Clara Rebeca |
| author_facet |
Parodi, Armando José Krisman de Fischman, Clara Rebeca |
| author_sort |
Parodi, Armando José |
| title |
In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| title_short |
In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| title_full |
In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| title_fullStr |
In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| title_full_unstemmed |
In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| title_sort |
in vitro synthesis of particulate glycogen from uridine diphosphate glucose |
| publishDate |
1969 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v132_n1_p111_Parodi http://hdl.handle.net/20.500.12110/paper_00039861_v132_n1_p111_Parodi |
| work_keys_str_mv |
AT parodiarmandojose invitrosynthesisofparticulateglycogenfromuridinediphosphateglucose AT krismandefischmanclararebeca invitrosynthesisofparticulateglycogenfromuridinediphosphateglucose |
| _version_ |
1768542864820666368 |