Guanosine diphosphate d-glucose glucohydrolase

A new enzyme has been found in yeast, which catalyzes the hydrolysis of guanosine diphosphate glucose to guanosine diphosphate and glucose. The enzyme was purified about 130-fold and its kinetic properties were studied. The enzymic activity seems to be strictly specific for guanosine diphosphate glu...

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Detalles Bibliográficos
Autores principales:	Carminatti, Héctor, Cabib, Enrique
Publicado:	1966
Materias:	glucose glycosidase guanine nucleotide magnesium mannose article chromatography electrophoresis enzymology gel chromatography kinetics metabolism Saccharomyces Chromatography Chromatography, Gel Electrophoresis Glucose Glycoside Hydrolases Guanine Nucleotides Kinetics Magnesium Mannose
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v116_nC_p26_Sonnino http://hdl.handle.net/20.500.12110/paper_00039861_v116_nC_p26_Sonnino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00039861_v116_nC_p26_Sonnino
record_format	dspace
spelling	paper:paper_00039861_v116_nC_p26_Sonnino2023-06-08T14:24:52Z Guanosine diphosphate d-glucose glucohydrolase Carminatti, Héctor Cabib, Enrique glucose glycosidase guanine nucleotide magnesium mannose article chromatography electrophoresis enzymology gel chromatography kinetics metabolism Saccharomyces Chromatography Chromatography, Gel Electrophoresis Glucose Glycoside Hydrolases Guanine Nucleotides Kinetics Magnesium Mannose Saccharomyces A new enzyme has been found in yeast, which catalyzes the hydrolysis of guanosine diphosphate glucose to guanosine diphosphate and glucose. The enzyme was purified about 130-fold and its kinetic properties were studied. The enzymic activity seems to be strictly specific for guanosine diphosphate glucose, with a Km of 0.23 mm. The enzyme is active over a wide range of pH values, from 5 to 9. Guanosine diphosphate inhibits competitively and Mg++ partially relieves the inhibition. The enzyme appears to exist in soluble form in the cytoplasm. The possible significance of guanosine diphosphate glucose glucohydrolase and of sugar nucleotide phosphorylase in the control of the intracellular concentration of guanosine diphosphate glucose and guanosine diphosphate mannose is discussed. © 1966. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cabib, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1966 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v116_nC_p26_Sonnino http://hdl.handle.net/20.500.12110/paper_00039861_v116_nC_p26_Sonnino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	glucose glycosidase guanine nucleotide magnesium mannose article chromatography electrophoresis enzymology gel chromatography kinetics metabolism Saccharomyces Chromatography Chromatography, Gel Electrophoresis Glucose Glycoside Hydrolases Guanine Nucleotides Kinetics Magnesium Mannose Saccharomyces
spellingShingle	glucose glycosidase guanine nucleotide magnesium mannose article chromatography electrophoresis enzymology gel chromatography kinetics metabolism Saccharomyces Chromatography Chromatography, Gel Electrophoresis Glucose Glycoside Hydrolases Guanine Nucleotides Kinetics Magnesium Mannose Saccharomyces Carminatti, Héctor Cabib, Enrique Guanosine diphosphate d-glucose glucohydrolase
topic_facet	glucose glycosidase guanine nucleotide magnesium mannose article chromatography electrophoresis enzymology gel chromatography kinetics metabolism Saccharomyces Chromatography Chromatography, Gel Electrophoresis Glucose Glycoside Hydrolases Guanine Nucleotides Kinetics Magnesium Mannose Saccharomyces
description	A new enzyme has been found in yeast, which catalyzes the hydrolysis of guanosine diphosphate glucose to guanosine diphosphate and glucose. The enzyme was purified about 130-fold and its kinetic properties were studied. The enzymic activity seems to be strictly specific for guanosine diphosphate glucose, with a Km of 0.23 mm. The enzyme is active over a wide range of pH values, from 5 to 9. Guanosine diphosphate inhibits competitively and Mg++ partially relieves the inhibition. The enzyme appears to exist in soluble form in the cytoplasm. The possible significance of guanosine diphosphate glucose glucohydrolase and of sugar nucleotide phosphorylase in the control of the intracellular concentration of guanosine diphosphate glucose and guanosine diphosphate mannose is discussed. © 1966.
author	Carminatti, Héctor Cabib, Enrique
author_facet	Carminatti, Héctor Cabib, Enrique
author_sort	Carminatti, Héctor
title	Guanosine diphosphate d-glucose glucohydrolase
title_short	Guanosine diphosphate d-glucose glucohydrolase
title_full	Guanosine diphosphate d-glucose glucohydrolase
title_fullStr	Guanosine diphosphate d-glucose glucohydrolase
title_full_unstemmed	Guanosine diphosphate d-glucose glucohydrolase
title_sort	guanosine diphosphate d-glucose glucohydrolase
publishDate	1966
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v116_nC_p26_Sonnino http://hdl.handle.net/20.500.12110/paper_00039861_v116_nC_p26_Sonnino
work_keys_str_mv	AT carminattihector guanosinediphosphatedglucoseglucohydrolase AT cabibenrique guanosinediphosphatedglucoseglucohydrolase
_version_	1768543012423467008

Guanosine diphosphate d-glucose glucohydrolase

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