Further studies on high molecular weight liver glycogen

A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a n...

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Detalles Bibliográficos
Autor principal:	Krisman de Fischman, Clara Rebeca
Publicado:	1966
Materias:	guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v113_n2_p265_Mordoh http://hdl.handle.net/20.500.12110/paper_00039861_v113_n2_p265_Mordoh
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00039861_v113_n2_p265_Mordoh
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spelling	paper:paper_00039861_v113_n2_p265_Mordoh2023-06-08T14:24:51Z Further studies on high molecular weight liver glycogen Krisman de Fischman, Clara Rebeca guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a new method and was found to remain constant under conditions in which glycogen content varied considerably. Native glycogen was compared with that prepared in vitro with purified enzymes. Although both preparations were similar in molecular weight and in their appearance under the electron microscope, they differed in the type of breakdown produced by acid, alkali, heat, and ultrasonic vibrations. When glycogen prepared in vitro was submitted to these procedures, its sedimentation coefficient decreased progressively, while native glycogen was broken down preferentially to a lighter population of about S = 100. © 1966. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1966 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v113_n2_p265_Mordoh http://hdl.handle.net/20.500.12110/paper_00039861_v113_n2_p265_Mordoh
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea
spellingShingle	guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea Krisman de Fischman, Clara Rebeca Further studies on high molecular weight liver glycogen
topic_facet	guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea
description	A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a new method and was found to remain constant under conditions in which glycogen content varied considerably. Native glycogen was compared with that prepared in vitro with purified enzymes. Although both preparations were similar in molecular weight and in their appearance under the electron microscope, they differed in the type of breakdown produced by acid, alkali, heat, and ultrasonic vibrations. When glycogen prepared in vitro was submitted to these procedures, its sedimentation coefficient decreased progressively, while native glycogen was broken down preferentially to a lighter population of about S = 100. © 1966.
author	Krisman de Fischman, Clara Rebeca
author_facet	Krisman de Fischman, Clara Rebeca
author_sort	Krisman de Fischman, Clara Rebeca
title	Further studies on high molecular weight liver glycogen
title_short	Further studies on high molecular weight liver glycogen
title_full	Further studies on high molecular weight liver glycogen
title_fullStr	Further studies on high molecular weight liver glycogen
title_full_unstemmed	Further studies on high molecular weight liver glycogen
title_sort	further studies on high molecular weight liver glycogen
publishDate	1966
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v113_n2_p265_Mordoh http://hdl.handle.net/20.500.12110/paper_00039861_v113_n2_p265_Mordoh
work_keys_str_mv	AT krismandefischmanclararebeca furtherstudiesonhighmolecularweightliverglycogen
_version_	1768542200376852480

Further studies on high molecular weight liver glycogen

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