Mechanism of glucose transfer from sucrose into the starch granule of sweet corn

The mechanism of sucrose-starch transformation was studied in corn endosperm. Preparations of endosperm which contain the total protein and starch granules catalyze an incorporation of radioactivity from sucrose-C14 to the granule in presence of ADP or UDP, ADP being more effective. No transfer of g...

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Autor principal:	Rongine, María Alejandra
Publicado:	1964
Materias:	adenine nucleotide adenosine triphosphate carbon glucose magnesium phosphotransferase pyrimidine nucleotide starch sucrose article carbohydrate metabolism cereal EXPERIMENTAL LAB STUDY grain research ADENINE NUCLEOTIDES ADENOSINE TRIPHOSPHATE CARBOHYDRATE METABOLISM CARBON ISOTOPES GLUCOSE GRAIN MAGNESIUM PHOSPHOTRANSFERASES STARCH SUCROSE URACIL NUCLEOTIDES Adenine Nucleotides Adenosine Triphosphate Carbohydrate Metabolism Carbon Isotopes Cereals Glucose Magnesium Phosphotransferases Research Starch Sucrose Uracil Nucleotides
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v104_n1_p173_RongineDeFekete http://hdl.handle.net/20.500.12110/paper_00039861_v104_n1_p173_RongineDeFekete
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00039861_v104_n1_p173_RongineDeFekete
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spelling	paper:paper_00039861_v104_n1_p173_RongineDeFekete2023-06-08T14:24:51Z Mechanism of glucose transfer from sucrose into the starch granule of sweet corn Rongine, María Alejandra adenine nucleotide adenosine triphosphate carbon glucose magnesium phosphotransferase pyrimidine nucleotide starch sucrose article carbohydrate metabolism cereal EXPERIMENTAL LAB STUDY grain research ADENINE NUCLEOTIDES ADENOSINE TRIPHOSPHATE CARBOHYDRATE METABOLISM CARBON ISOTOPES EXPERIMENTAL LAB STUDY GLUCOSE GRAIN MAGNESIUM PHOSPHOTRANSFERASES STARCH SUCROSE URACIL NUCLEOTIDES Adenine Nucleotides Adenosine Triphosphate Carbohydrate Metabolism Carbon Isotopes Cereals Glucose Magnesium Phosphotransferases Research Starch Sucrose Uracil Nucleotides The mechanism of sucrose-starch transformation was studied in corn endosperm. Preparations of endosperm which contain the total protein and starch granules catalyze an incorporation of radioactivity from sucrose-C14 to the granule in presence of ADP or UDP, ADP being more effective. No transfer of glucose-C14 from glucose-1-P to the granule was observed unless Mg++ and ATP were present. The Km values of the UDP-glucose-fructose glucosyl transferase for ADP, UDP, ADP-glucose, and UDP-glucose and their reciprocal inhibitions were measured From all these results the following two sequences for the glucose incorporation into the granule were proposed: (a) SucroseαADP-glucose (or UDP-glucose)→starch (b) SucroseαUDP-glucoseαglucose-1-PαADP-glucose→starch. The enzymes which catalyze all these steps could be demonstrated in corn endosperm. © 1964 Academic Press Inc. All rights reserved. Fil:Rongine De Fekete, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1964 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v104_n1_p173_RongineDeFekete http://hdl.handle.net/20.500.12110/paper_00039861_v104_n1_p173_RongineDeFekete
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenine nucleotide adenosine triphosphate carbon glucose magnesium phosphotransferase pyrimidine nucleotide starch sucrose article carbohydrate metabolism cereal EXPERIMENTAL LAB STUDY grain research ADENINE NUCLEOTIDES ADENOSINE TRIPHOSPHATE CARBOHYDRATE METABOLISM CARBON ISOTOPES EXPERIMENTAL LAB STUDY GLUCOSE GRAIN MAGNESIUM PHOSPHOTRANSFERASES STARCH SUCROSE URACIL NUCLEOTIDES Adenine Nucleotides Adenosine Triphosphate Carbohydrate Metabolism Carbon Isotopes Cereals Glucose Magnesium Phosphotransferases Research Starch Sucrose Uracil Nucleotides
spellingShingle	adenine nucleotide adenosine triphosphate carbon glucose magnesium phosphotransferase pyrimidine nucleotide starch sucrose article carbohydrate metabolism cereal EXPERIMENTAL LAB STUDY grain research ADENINE NUCLEOTIDES ADENOSINE TRIPHOSPHATE CARBOHYDRATE METABOLISM CARBON ISOTOPES EXPERIMENTAL LAB STUDY GLUCOSE GRAIN MAGNESIUM PHOSPHOTRANSFERASES STARCH SUCROSE URACIL NUCLEOTIDES Adenine Nucleotides Adenosine Triphosphate Carbohydrate Metabolism Carbon Isotopes Cereals Glucose Magnesium Phosphotransferases Research Starch Sucrose Uracil Nucleotides Rongine, María Alejandra Mechanism of glucose transfer from sucrose into the starch granule of sweet corn
topic_facet	adenine nucleotide adenosine triphosphate carbon glucose magnesium phosphotransferase pyrimidine nucleotide starch sucrose article carbohydrate metabolism cereal EXPERIMENTAL LAB STUDY grain research ADENINE NUCLEOTIDES ADENOSINE TRIPHOSPHATE CARBOHYDRATE METABOLISM CARBON ISOTOPES EXPERIMENTAL LAB STUDY GLUCOSE GRAIN MAGNESIUM PHOSPHOTRANSFERASES STARCH SUCROSE URACIL NUCLEOTIDES Adenine Nucleotides Adenosine Triphosphate Carbohydrate Metabolism Carbon Isotopes Cereals Glucose Magnesium Phosphotransferases Research Starch Sucrose Uracil Nucleotides
description	The mechanism of sucrose-starch transformation was studied in corn endosperm. Preparations of endosperm which contain the total protein and starch granules catalyze an incorporation of radioactivity from sucrose-C14 to the granule in presence of ADP or UDP, ADP being more effective. No transfer of glucose-C14 from glucose-1-P to the granule was observed unless Mg++ and ATP were present. The Km values of the UDP-glucose-fructose glucosyl transferase for ADP, UDP, ADP-glucose, and UDP-glucose and their reciprocal inhibitions were measured From all these results the following two sequences for the glucose incorporation into the granule were proposed: (a) SucroseαADP-glucose (or UDP-glucose)→starch (b) SucroseαUDP-glucoseαglucose-1-PαADP-glucose→starch. The enzymes which catalyze all these steps could be demonstrated in corn endosperm. © 1964 Academic Press Inc. All rights reserved.
author	Rongine, María Alejandra
author_facet	Rongine, María Alejandra
author_sort	Rongine, María Alejandra
title	Mechanism of glucose transfer from sucrose into the starch granule of sweet corn
title_short	Mechanism of glucose transfer from sucrose into the starch granule of sweet corn
title_full	Mechanism of glucose transfer from sucrose into the starch granule of sweet corn
title_fullStr	Mechanism of glucose transfer from sucrose into the starch granule of sweet corn
title_full_unstemmed	Mechanism of glucose transfer from sucrose into the starch granule of sweet corn
title_sort	mechanism of glucose transfer from sucrose into the starch granule of sweet corn
publishDate	1964
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v104_n1_p173_RongineDeFekete http://hdl.handle.net/20.500.12110/paper_00039861_v104_n1_p173_RongineDeFekete
work_keys_str_mv	AT ronginemariaalejandra mechanismofglucosetransferfromsucroseintothestarchgranuleofsweetcorn
_version_	1768543491964534784

Mechanism of glucose transfer from sucrose into the starch granule of sweet corn

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