Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry

In this report, we developed the pressure probe electrospray ionization-mass spectrometry with internal electrode capillary (IEC-PPESI-MS) which enables high spatial-resolution cell sampling, precise postsampling manipulation, and high detection sensitivity. Using this technique, a comparative in si...

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Detalles Bibliográficos
Autor principal: Erra Balsells, Rosa
Publicado: 2016
Materias:
Biomolecules
Electrodes
Electrospray ionization
Flavonoids
Fruits
Ionization
Mass spectrometry
Metabolism
Metabolites
Plants (botany)
Potential flow
Probes
Spectrometry
Cell-to-cell variation
Detection sensitivity
High spatial resolution
Internal electrodes
Metabolite profiles
Physicochemical property
Sequential analysis
Solanum lycopersicum
Molecular biology
electrode
electrospray mass spectrometry
metabolism
procedures
trichome
Spectrometry, Mass, Electrospray Ionization
Trichomes
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v88_n6_p3049_Nakashima
http://hdl.handle.net/20.500.12110/paper_00032700_v88_n6_p3049_Nakashima
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00032700_v88_n6_p3049_Nakashima
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spelling paper:paper_00032700_v88_n6_p3049_Nakashima2023-06-08T14:24:15Z Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry Erra Balsells, Rosa Biomolecules Electrodes Electrospray ionization Flavonoids Fruits Ionization Mass spectrometry Metabolism Metabolites Plants (botany) Potential flow Probes Spectrometry Cell-to-cell variation Detection sensitivity High spatial resolution Internal electrodes Metabolite profiles Physicochemical property Sequential analysis Solanum lycopersicum Molecular biology electrode electrospray mass spectrometry metabolism procedures trichome Electrodes Spectrometry, Mass, Electrospray Ionization Trichomes In this report, we developed the pressure probe electrospray ionization-mass spectrometry with internal electrode capillary (IEC-PPESI-MS) which enables high spatial-resolution cell sampling, precise postsampling manipulation, and high detection sensitivity. Using this technique, a comparative in situ single-cell metabolite profiling of stalk and glandular cells, the two adjacent cell types comprising a trichome unit in tomato plants (Solanum lycopersicum L.), were performed to clarify the extent of metabolic differentiation between two cell types as well as among different types of trichomes. Owing to high sensitivity of the system, less than a picoliter cell sap from a single stalk cell sufficiently yielded a number of peaks of amino acids, organic acids, carbohydrates, and flavonoids. The minimal cell sap removal from a stalk cell without severe disturbance of trichome structure enabled sequential analysis of adjacent glandular cell on the same trichome, which showed the presence of striking differences in metabolite compositions between two adjacent cell types. Comparison among different types of trichome also revealed significant variations in metabolite profiles, particularly in flavonoids and acyl sugars compositions. Some metabolites were found only in specific cell types or particular trichome types. Although extensive metabolomics analysis of glandular cells of tomato trichomes has been previously documented, this is the first report describing cell-to-cell variations in metabolite compositions of stalk and glandular cells as well as in different trichome types. Further application of this technique may provide new insights into distinct metabolism in plant cells displaying variations in shape, size, function and physicochemical properties. (Figure Presented). © 2016 American Chemical Society. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v88_n6_p3049_Nakashima http://hdl.handle.net/20.500.12110/paper_00032700_v88_n6_p3049_Nakashima
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biomolecules
Electrodes
Electrospray ionization
Flavonoids
Fruits
Ionization
Mass spectrometry
Metabolism
Metabolites
Plants (botany)
Potential flow
Probes
Spectrometry
Cell-to-cell variation
Detection sensitivity
High spatial resolution
Internal electrodes
Metabolite profiles
Physicochemical property
Sequential analysis
Solanum lycopersicum
Molecular biology
electrode
electrospray mass spectrometry
metabolism
procedures
trichome
Electrodes
Spectrometry, Mass, Electrospray Ionization
Trichomes
spellingShingle Biomolecules
Electrodes
Electrospray ionization
Flavonoids
Fruits
Ionization
Mass spectrometry
Metabolism
Metabolites
Plants (botany)
Potential flow
Probes
Spectrometry
Cell-to-cell variation
Detection sensitivity
High spatial resolution
Internal electrodes
Metabolite profiles
Physicochemical property
Sequential analysis
Solanum lycopersicum
Molecular biology
electrode
electrospray mass spectrometry
metabolism
procedures
trichome
Electrodes
Spectrometry, Mass, Electrospray Ionization
Trichomes
Erra Balsells, Rosa
Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry
topic_facet Biomolecules
Electrodes
Electrospray ionization
Flavonoids
Fruits
Ionization
Mass spectrometry
Metabolism
Metabolites
Plants (botany)
Potential flow
Probes
Spectrometry
Cell-to-cell variation
Detection sensitivity
High spatial resolution
Internal electrodes
Metabolite profiles
Physicochemical property
Sequential analysis
Solanum lycopersicum
Molecular biology
electrode
electrospray mass spectrometry
metabolism
procedures
trichome
Electrodes
Spectrometry, Mass, Electrospray Ionization
Trichomes
description In this report, we developed the pressure probe electrospray ionization-mass spectrometry with internal electrode capillary (IEC-PPESI-MS) which enables high spatial-resolution cell sampling, precise postsampling manipulation, and high detection sensitivity. Using this technique, a comparative in situ single-cell metabolite profiling of stalk and glandular cells, the two adjacent cell types comprising a trichome unit in tomato plants (Solanum lycopersicum L.), were performed to clarify the extent of metabolic differentiation between two cell types as well as among different types of trichomes. Owing to high sensitivity of the system, less than a picoliter cell sap from a single stalk cell sufficiently yielded a number of peaks of amino acids, organic acids, carbohydrates, and flavonoids. The minimal cell sap removal from a stalk cell without severe disturbance of trichome structure enabled sequential analysis of adjacent glandular cell on the same trichome, which showed the presence of striking differences in metabolite compositions between two adjacent cell types. Comparison among different types of trichome also revealed significant variations in metabolite profiles, particularly in flavonoids and acyl sugars compositions. Some metabolites were found only in specific cell types or particular trichome types. Although extensive metabolomics analysis of glandular cells of tomato trichomes has been previously documented, this is the first report describing cell-to-cell variations in metabolite compositions of stalk and glandular cells as well as in different trichome types. Further application of this technique may provide new insights into distinct metabolism in plant cells displaying variations in shape, size, function and physicochemical properties. (Figure Presented). © 2016 American Chemical Society.
author Erra Balsells, Rosa
author_facet Erra Balsells, Rosa
author_sort Erra Balsells, Rosa
title Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry
title_short Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry
title_full Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry
title_fullStr Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry
title_full_unstemmed Single-Cell Metabolite Profiling of Stalk and Glandular Cells of Intact Trichomes with Internal Electrode Capillary Pressure Probe Electrospray Ionization Mass Spectrometry
title_sort single-cell metabolite profiling of stalk and glandular cells of intact trichomes with internal electrode capillary pressure probe electrospray ionization mass spectrometry
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v88_n6_p3049_Nakashima
http://hdl.handle.net/20.500.12110/paper_00032700_v88_n6_p3049_Nakashima
work_keys_str_mv AT errabalsellsrosa singlecellmetaboliteprofilingofstalkandglandularcellsofintacttrichomeswithinternalelectrodecapillarypressureprobeelectrosprayionizationmassspectrometry
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