Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level

Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its dono...

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Autores principales:	Suarez, Sebastian, Bikiel, Damian Ezequiel, Wetzler, Diana E., Martí, Marcelo Adrián, Doctorovich, Fabio Ariel
Publicado:	2013
Materias:	Analytical method Chemical and biologicals Covalent attachment Electrode response Pharmacological properties Reactive nitrogen and oxygen species Signaling molecules Time-resolved detection Cell culture Chemical reactions Gold compounds High performance liquid chromatography Mass spectrometry Cobalt compounds nitrogen oxide nitroxyl article electrochemical analysis fluorescence high performance liquid chromatography kinetics limit of detection mass spectrometry methodology ultraviolet spectrophotometry Chromatography, High Pressure Liquid Electrochemical Techniques Fluorescence Kinetics Limit of Detection Mass Spectrometry Nitrogen Oxides Spectrophotometry, Ultraviolet
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v85_n21_p10262_Suarez http://hdl.handle.net/20.500.12110/paper_00032700_v85_n21_p10262_Suarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00032700_v85_n21_p10262_Suarez
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spelling	paper:paper_00032700_v85_n21_p10262_Suarez2023-06-08T14:24:13Z Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level Suarez, Sebastian Bikiel, Damian Ezequiel Wetzler, Diana E. Martí, Marcelo Adrián Doctorovich, Fabio Ariel Analytical method Chemical and biologicals Covalent attachment Electrode response Pharmacological properties Reactive nitrogen and oxygen species Signaling molecules Time-resolved detection Cell culture Chemical reactions Gold compounds High performance liquid chromatography Mass spectrometry Cobalt compounds nitrogen oxide nitroxyl article electrochemical analysis fluorescence high performance liquid chromatography kinetics limit of detection mass spectrometry methodology ultraviolet spectrophotometry Chromatography, High Pressure Liquid Electrochemical Techniques Fluorescence Kinetics Limit of Detection Mass Spectrometry Nitrogen Oxides Spectrophotometry, Ultraviolet Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its donors have important pharmacological properties. Therefore, given its relevance and elusive nature (it reacts with itself very quickly), the development of reliable analytical methods for quantitative HNO detection is in high demand for the advancement of future research in this area. During the past few years, several methods were developed that rely on chemical reactions followed by mass spectrometry, high-performance liquid chromatography, UV-vis, or fluorescence-trapping-based methodologies. In this work, our recently developed HNO-sensing electrode, based on the covalent attachment of cobalt(II) 5,10,15,20-tetrakis[3-(p-acetylthiopropoxy)phenyl] porphyrin [Co(P)] to a gold electrode, has been thoroughly characterized in terms of sensibility, accuracy, time-resolved detection, and compatibility with complex biologically compatible media. Our results show that the Co(P) electrode: (i) allows time-resolved detection and kinetic analysis of the electrode response (the underlying HNO-producing reactions can be characterized) (ii) is able to selectively detect and reliably quantify HNO in the 1-1000 nM range, and (iii) has good biological media compatibility (including cell culture), displaying a lack of spurious signals due to the presence of O2, NO, and other reactive nitrogen and oxygen species. In summary, the Co(P) electrode is to our knowledge the best prospect for use in studies investigating HNO-related chemical and biological reactions. © 2013 American Chemical Society. Fil:Suárez, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bikiel, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wetzler, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martí, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Doctorovich, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v85_n21_p10262_Suarez http://hdl.handle.net/20.500.12110/paper_00032700_v85_n21_p10262_Suarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Analytical method Chemical and biologicals Covalent attachment Electrode response Pharmacological properties Reactive nitrogen and oxygen species Signaling molecules Time-resolved detection Cell culture Chemical reactions Gold compounds High performance liquid chromatography Mass spectrometry Cobalt compounds nitrogen oxide nitroxyl article electrochemical analysis fluorescence high performance liquid chromatography kinetics limit of detection mass spectrometry methodology ultraviolet spectrophotometry Chromatography, High Pressure Liquid Electrochemical Techniques Fluorescence Kinetics Limit of Detection Mass Spectrometry Nitrogen Oxides Spectrophotometry, Ultraviolet
spellingShingle	Analytical method Chemical and biologicals Covalent attachment Electrode response Pharmacological properties Reactive nitrogen and oxygen species Signaling molecules Time-resolved detection Cell culture Chemical reactions Gold compounds High performance liquid chromatography Mass spectrometry Cobalt compounds nitrogen oxide nitroxyl article electrochemical analysis fluorescence high performance liquid chromatography kinetics limit of detection mass spectrometry methodology ultraviolet spectrophotometry Chromatography, High Pressure Liquid Electrochemical Techniques Fluorescence Kinetics Limit of Detection Mass Spectrometry Nitrogen Oxides Spectrophotometry, Ultraviolet Suarez, Sebastian Bikiel, Damian Ezequiel Wetzler, Diana E. Martí, Marcelo Adrián Doctorovich, Fabio Ariel Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
topic_facet	Analytical method Chemical and biologicals Covalent attachment Electrode response Pharmacological properties Reactive nitrogen and oxygen species Signaling molecules Time-resolved detection Cell culture Chemical reactions Gold compounds High performance liquid chromatography Mass spectrometry Cobalt compounds nitrogen oxide nitroxyl article electrochemical analysis fluorescence high performance liquid chromatography kinetics limit of detection mass spectrometry methodology ultraviolet spectrophotometry Chromatography, High Pressure Liquid Electrochemical Techniques Fluorescence Kinetics Limit of Detection Mass Spectrometry Nitrogen Oxides Spectrophotometry, Ultraviolet
description	Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its donors have important pharmacological properties. Therefore, given its relevance and elusive nature (it reacts with itself very quickly), the development of reliable analytical methods for quantitative HNO detection is in high demand for the advancement of future research in this area. During the past few years, several methods were developed that rely on chemical reactions followed by mass spectrometry, high-performance liquid chromatography, UV-vis, or fluorescence-trapping-based methodologies. In this work, our recently developed HNO-sensing electrode, based on the covalent attachment of cobalt(II) 5,10,15,20-tetrakis[3-(p-acetylthiopropoxy)phenyl] porphyrin [Co(P)] to a gold electrode, has been thoroughly characterized in terms of sensibility, accuracy, time-resolved detection, and compatibility with complex biologically compatible media. Our results show that the Co(P) electrode: (i) allows time-resolved detection and kinetic analysis of the electrode response (the underlying HNO-producing reactions can be characterized) (ii) is able to selectively detect and reliably quantify HNO in the 1-1000 nM range, and (iii) has good biological media compatibility (including cell culture), displaying a lack of spurious signals due to the presence of O2, NO, and other reactive nitrogen and oxygen species. In summary, the Co(P) electrode is to our knowledge the best prospect for use in studies investigating HNO-related chemical and biological reactions. © 2013 American Chemical Society.
author	Suarez, Sebastian Bikiel, Damian Ezequiel Wetzler, Diana E. Martí, Marcelo Adrián Doctorovich, Fabio Ariel
author_facet	Suarez, Sebastian Bikiel, Damian Ezequiel Wetzler, Diana E. Martí, Marcelo Adrián Doctorovich, Fabio Ariel
author_sort	Suarez, Sebastian
title	Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_short	Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_full	Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_fullStr	Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_full_unstemmed	Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_sort	time-resolved electrochemical quantification of azanone (hno) at low nanomolar level
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v85_n21_p10262_Suarez http://hdl.handle.net/20.500.12110/paper_00032700_v85_n21_p10262_Suarez
work_keys_str_mv	AT suarezsebastian timeresolvedelectrochemicalquantificationofazanonehnoatlownanomolarlevel AT bikieldamianezequiel timeresolvedelectrochemicalquantificationofazanonehnoatlownanomolarlevel AT wetzlerdianae timeresolvedelectrochemicalquantificationofazanonehnoatlownanomolarlevel AT martimarceloadrian timeresolvedelectrochemicalquantificationofazanonehnoatlownanomolarlevel AT doctorovichfabioariel timeresolvedelectrochemicalquantificationofazanonehnoatlownanomolarlevel
_version_	1768544205052837888

Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level

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