Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinica...
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2012
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v87_n2_p267_Longhi http://hdl.handle.net/20.500.12110/paper_00029637_v87_n2_p267_Longhi |
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paper:paper_00029637_v87_n2_p267_Longhi2023-06-08T14:23:21Z Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms bacterium lysate peptide peptide jl18 peptide jl19 peptide p013 peptide p0beta peptide r13 recombinant antigen recombinant protein recombinant protein jl7 unclassified drug adolescent adult article blood sampling Brazil Chagas disease child controlled study cross reaction disease severity enzyme linked immunosorbent assay female human immunofluorescence major clinical study male school child sensitivity and specificity serology Trypanosoma cruzi visceral leishmaniasis Adolescent Adult Animals Antibodies, Protozoan Antigens, Protozoan Brazil Chagas Disease Child Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Recombinant Proteins Sensitivity and Specificity Trypanosoma cruzi Young Adult The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v87_n2_p267_Longhi http://hdl.handle.net/20.500.12110/paper_00029637_v87_n2_p267_Longhi |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
bacterium lysate peptide peptide jl18 peptide jl19 peptide p013 peptide p0beta peptide r13 recombinant antigen recombinant protein recombinant protein jl7 unclassified drug adolescent adult article blood sampling Brazil Chagas disease child controlled study cross reaction disease severity enzyme linked immunosorbent assay female human immunofluorescence major clinical study male school child sensitivity and specificity serology Trypanosoma cruzi visceral leishmaniasis Adolescent Adult Animals Antibodies, Protozoan Antigens, Protozoan Brazil Chagas Disease Child Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Recombinant Proteins Sensitivity and Specificity Trypanosoma cruzi Young Adult |
spellingShingle |
bacterium lysate peptide peptide jl18 peptide jl19 peptide p013 peptide p0beta peptide r13 recombinant antigen recombinant protein recombinant protein jl7 unclassified drug adolescent adult article blood sampling Brazil Chagas disease child controlled study cross reaction disease severity enzyme linked immunosorbent assay female human immunofluorescence major clinical study male school child sensitivity and specificity serology Trypanosoma cruzi visceral leishmaniasis Adolescent Adult Animals Antibodies, Protozoan Antigens, Protozoan Brazil Chagas Disease Child Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Recombinant Proteins Sensitivity and Specificity Trypanosoma cruzi Young Adult Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
topic_facet |
bacterium lysate peptide peptide jl18 peptide jl19 peptide p013 peptide p0beta peptide r13 recombinant antigen recombinant protein recombinant protein jl7 unclassified drug adolescent adult article blood sampling Brazil Chagas disease child controlled study cross reaction disease severity enzyme linked immunosorbent assay female human immunofluorescence major clinical study male school child sensitivity and specificity serology Trypanosoma cruzi visceral leishmaniasis Adolescent Adult Animals Antibodies, Protozoan Antigens, Protozoan Brazil Chagas Disease Child Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Recombinant Proteins Sensitivity and Specificity Trypanosoma cruzi Young Adult |
description |
The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene. |
title |
Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
title_short |
Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
title_full |
Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
title_fullStr |
Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
title_full_unstemmed |
Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
title_sort |
evaluation of in-house elisa using trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms |
publishDate |
2012 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v87_n2_p267_Longhi http://hdl.handle.net/20.500.12110/paper_00029637_v87_n2_p267_Longhi |
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1768546374681362432 |