Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS

Trypanosoma cruzi DNA was amplified from an intracranial biopsy and peripheral blood of an HIV patient with encephalitis; this episode was indicative of AIDS and congenital Chagas disease. The analysis of a microsatellite locus revealed a multiclonal parasite population at the brain lesion with a mo...

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Autores principales:	Burgos, Juan Miguel, Duffy, Tomás, Schijman, Alejandro Gabriel
Publicado:	2005
Materias:	antiparasitic agent antiretrovirus agent benznidazole protozoal DNA ribosomal DNA 24salpha ribosome DNA unclassified drug acquired immune deficiency syndrome adult article brain biopsy case report Chagas disease clone diagnostic procedure gene amplification human male microorganism detection molecular typing nonhuman polymerase chain reaction quantitative analysis restriction fragment length polymorphism survival Trypanosoma cruzi virus encephalitis Adult AIDS-Related Opportunistic Infections Animals Chagas Disease DNA, Kinetoplast DNA, Protozoan Encephalitis Humans Magnetic Resonance Imaging Male Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Variation (Genetics)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos http://hdl.handle.net/20.500.12110/paper_00029637_v73_n6_p1016_Burgos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00029637_v73_n6_p1016_Burgos
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spelling	paper:paper_00029637_v73_n6_p1016_Burgos2023-06-08T14:23:19Z Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS Burgos, Juan Miguel Duffy, Tomás Schijman, Alejandro Gabriel antiparasitic agent antiretrovirus agent benznidazole protozoal DNA ribosomal DNA 24salpha ribosome DNA unclassified drug acquired immune deficiency syndrome adult article brain biopsy case report Chagas disease clone diagnostic procedure gene amplification human male microorganism detection molecular typing nonhuman polymerase chain reaction quantitative analysis restriction fragment length polymorphism survival Trypanosoma cruzi virus encephalitis Adult AIDS-Related Opportunistic Infections Animals Chagas Disease DNA, Kinetoplast DNA, Protozoan Encephalitis Humans Magnetic Resonance Imaging Male Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Trypanosoma cruzi Variation (Genetics) Trypanosoma cruzi Trypanosoma cruzi DNA was amplified from an intracranial biopsy and peripheral blood of an HIV patient with encephalitis; this episode was indicative of AIDS and congenital Chagas disease. The analysis of a microsatellite locus revealed a multiclonal parasite population at the brain lesion with a more complex minicircle signature than that profiled in blood using restriction fragment length polymorphism (RFLP)-PCR and low stringency single primer (LSSP) PCR. Interestingly, different sublineages of T. cruzi II were detected in blood and brain by means of spliced-leader and 24sα ribosomal-DNA amplifications. Quantitative-competitive PCR monitored the decrease of parasitic load during treatment and secondary prophylaxis with benznidazole. The synergy between parasiticidal plus antiretroviral treatments probably allowed the patient a longer survival than usually achieved in similar episodes. This is the first case report demonstrating a differential distribution of natural parasite populations and sublineages in Chagas disease reactivation, showing the proliferation of cerebral variants not detectable in peripheral blood. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene. Fil:Burgos, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Duffy, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos http://hdl.handle.net/20.500.12110/paper_00029637_v73_n6_p1016_Burgos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	antiparasitic agent antiretrovirus agent benznidazole protozoal DNA ribosomal DNA 24salpha ribosome DNA unclassified drug acquired immune deficiency syndrome adult article brain biopsy case report Chagas disease clone diagnostic procedure gene amplification human male microorganism detection molecular typing nonhuman polymerase chain reaction quantitative analysis restriction fragment length polymorphism survival Trypanosoma cruzi virus encephalitis Adult AIDS-Related Opportunistic Infections Animals Chagas Disease DNA, Kinetoplast DNA, Protozoan Encephalitis Humans Magnetic Resonance Imaging Male Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Trypanosoma cruzi Variation (Genetics) Trypanosoma cruzi
spellingShingle	antiparasitic agent antiretrovirus agent benznidazole protozoal DNA ribosomal DNA 24salpha ribosome DNA unclassified drug acquired immune deficiency syndrome adult article brain biopsy case report Chagas disease clone diagnostic procedure gene amplification human male microorganism detection molecular typing nonhuman polymerase chain reaction quantitative analysis restriction fragment length polymorphism survival Trypanosoma cruzi virus encephalitis Adult AIDS-Related Opportunistic Infections Animals Chagas Disease DNA, Kinetoplast DNA, Protozoan Encephalitis Humans Magnetic Resonance Imaging Male Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Trypanosoma cruzi Variation (Genetics) Trypanosoma cruzi Burgos, Juan Miguel Duffy, Tomás Schijman, Alejandro Gabriel Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS
topic_facet	antiparasitic agent antiretrovirus agent benznidazole protozoal DNA ribosomal DNA 24salpha ribosome DNA unclassified drug acquired immune deficiency syndrome adult article brain biopsy case report Chagas disease clone diagnostic procedure gene amplification human male microorganism detection molecular typing nonhuman polymerase chain reaction quantitative analysis restriction fragment length polymorphism survival Trypanosoma cruzi virus encephalitis Adult AIDS-Related Opportunistic Infections Animals Chagas Disease DNA, Kinetoplast DNA, Protozoan Encephalitis Humans Magnetic Resonance Imaging Male Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Trypanosoma cruzi Variation (Genetics) Trypanosoma cruzi
description	Trypanosoma cruzi DNA was amplified from an intracranial biopsy and peripheral blood of an HIV patient with encephalitis; this episode was indicative of AIDS and congenital Chagas disease. The analysis of a microsatellite locus revealed a multiclonal parasite population at the brain lesion with a more complex minicircle signature than that profiled in blood using restriction fragment length polymorphism (RFLP)-PCR and low stringency single primer (LSSP) PCR. Interestingly, different sublineages of T. cruzi II were detected in blood and brain by means of spliced-leader and 24sα ribosomal-DNA amplifications. Quantitative-competitive PCR monitored the decrease of parasitic load during treatment and secondary prophylaxis with benznidazole. The synergy between parasiticidal plus antiretroviral treatments probably allowed the patient a longer survival than usually achieved in similar episodes. This is the first case report demonstrating a differential distribution of natural parasite populations and sublineages in Chagas disease reactivation, showing the proliferation of cerebral variants not detectable in peripheral blood. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.
author	Burgos, Juan Miguel Duffy, Tomás Schijman, Alejandro Gabriel
author_facet	Burgos, Juan Miguel Duffy, Tomás Schijman, Alejandro Gabriel
author_sort	Burgos, Juan Miguel
title	Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS
title_short	Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS
title_full	Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS
title_fullStr	Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS
title_full_unstemmed	Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS
title_sort	molecular diagnosis and typing of trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with aids
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v73_n6_p1016_Burgos http://hdl.handle.net/20.500.12110/paper_00029637_v73_n6_p1016_Burgos
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Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with AIDS

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