White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression

Premise of the study: Duchesnea indica is a wild strawberry-like species that has red fruits. In a recent survey in the highlands of Tucum á n (Argentina), a plant of D. indica with white fruits was discovered. The aim of this study was to investigate whether the white-fruited character was due to a...

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Autor principal:	Wulff, Arturo Federico
Publicado:	2011
Materias:	ANS Anthocyanin Gene expression Intra-specific cross MYB10 Rosaceae White-fruited Duchesnea indica anthocyanin vegetable protein color fruit gene expression genotype germination heritability horticulture life history trait molecular analysis phenotype pigment taxonomy wild population article biosynthesis cross breeding flower gene expression regulation genetics histology metabolism pigmentation plant gene plant leaf species difference Anthocyanins Crosses, Genetic Flowers Fruit Gene Expression Regulation, Plant Genes, Plant Genotype Pigmentation Plant Leaves Plant Proteins Species Specificity Argentina Tucuman Duchesnea indica Fragaria x ananassa
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029122_v98_n12_p2077_Debes http://hdl.handle.net/20.500.12110/paper_00029122_v98_n12_p2077_Debes
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00029122_v98_n12_p2077_Debes
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spelling	paper:paper_00029122_v98_n12_p2077_Debes2023-06-08T14:23:04Z White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression Wulff, Arturo Federico ANS Anthocyanin Gene expression Intra-specific cross MYB10 Rosaceae White-fruited Duchesnea indica anthocyanin vegetable protein color fruit gene expression genotype germination heritability horticulture life history trait molecular analysis phenotype pigment taxonomy wild population article biosynthesis cross breeding flower fruit gene expression regulation genetics genotype histology metabolism pigmentation plant gene plant leaf Rosaceae species difference Anthocyanins Crosses, Genetic Flowers Fruit Gene Expression Regulation, Plant Genes, Plant Genotype Pigmentation Plant Leaves Plant Proteins Rosaceae Species Specificity Argentina Tucuman Duchesnea indica Fragaria x ananassa Rosaceae Premise of the study: Duchesnea indica is a wild strawberry-like species that has red fruits. In a recent survey in the highlands of Tucum á n (Argentina), a plant of D. indica with white fruits was discovered. The aim of this study was to investigate whether the white-fruited character was due to a phenotypic or genotypic change. The stability and heritability of the character and the expression of genes involved in anthocyanins synthesis were studied and compared with red-fruited genotypes. This study contributes to understanding the molecular basis of some factors involved in fruit pigmentation, a horticulturally and taxonomically important trait. Methods: Stability and heritability of the white-fruited character were evaluated in plants obtained by asexual propagation or by sexual crosses between the white- and red-fruited genotypes. Asexual multiplications were carried out by stolon rooting and sexual multiplications by germination of achenes obtained from crosses. The expression level of the genes involved in the synthesis and regulation of the anthocyanins pathway (CHS, F3H, DFR, ANS, and MYB10) were evaluated by RT-PCR using specific primers. Key results: Plants with the white-fruited character always yielded white-fruited progeny when propagated asexually, whereas in sexually propagated plants fruit color depended on the mother. Red-fruited mothers yielded red-fruited progeny, and whitefruited mothers yielded fruits ranging from dark pink to white. Molecular analysis suggested that the white-fruited character was due to the low expression of the ANS gene. Conclusions: Results obtained indicate that the white-fruited character was stable. Mother progenitors exert a strong influence on the expression of the white-fruited character. The white-fruited phenotype is due to the impairment or downregulation of the ANS gene. © 2011 Botanical Society of America. Fil:Wulff, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029122_v98_n12_p2077_Debes http://hdl.handle.net/20.500.12110/paper_00029122_v98_n12_p2077_Debes
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ANS Anthocyanin Gene expression Intra-specific cross MYB10 Rosaceae White-fruited Duchesnea indica anthocyanin vegetable protein color fruit gene expression genotype germination heritability horticulture life history trait molecular analysis phenotype pigment taxonomy wild population article biosynthesis cross breeding flower fruit gene expression regulation genetics genotype histology metabolism pigmentation plant gene plant leaf Rosaceae species difference Anthocyanins Crosses, Genetic Flowers Fruit Gene Expression Regulation, Plant Genes, Plant Genotype Pigmentation Plant Leaves Plant Proteins Rosaceae Species Specificity Argentina Tucuman Duchesnea indica Fragaria x ananassa Rosaceae
spellingShingle	ANS Anthocyanin Gene expression Intra-specific cross MYB10 Rosaceae White-fruited Duchesnea indica anthocyanin vegetable protein color fruit gene expression genotype germination heritability horticulture life history trait molecular analysis phenotype pigment taxonomy wild population article biosynthesis cross breeding flower fruit gene expression regulation genetics genotype histology metabolism pigmentation plant gene plant leaf Rosaceae species difference Anthocyanins Crosses, Genetic Flowers Fruit Gene Expression Regulation, Plant Genes, Plant Genotype Pigmentation Plant Leaves Plant Proteins Rosaceae Species Specificity Argentina Tucuman Duchesnea indica Fragaria x ananassa Rosaceae Wulff, Arturo Federico White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
topic_facet	ANS Anthocyanin Gene expression Intra-specific cross MYB10 Rosaceae White-fruited Duchesnea indica anthocyanin vegetable protein color fruit gene expression genotype germination heritability horticulture life history trait molecular analysis phenotype pigment taxonomy wild population article biosynthesis cross breeding flower fruit gene expression regulation genetics genotype histology metabolism pigmentation plant gene plant leaf Rosaceae species difference Anthocyanins Crosses, Genetic Flowers Fruit Gene Expression Regulation, Plant Genes, Plant Genotype Pigmentation Plant Leaves Plant Proteins Rosaceae Species Specificity Argentina Tucuman Duchesnea indica Fragaria x ananassa Rosaceae
description	Premise of the study: Duchesnea indica is a wild strawberry-like species that has red fruits. In a recent survey in the highlands of Tucum á n (Argentina), a plant of D. indica with white fruits was discovered. The aim of this study was to investigate whether the white-fruited character was due to a phenotypic or genotypic change. The stability and heritability of the character and the expression of genes involved in anthocyanins synthesis were studied and compared with red-fruited genotypes. This study contributes to understanding the molecular basis of some factors involved in fruit pigmentation, a horticulturally and taxonomically important trait. Methods: Stability and heritability of the white-fruited character were evaluated in plants obtained by asexual propagation or by sexual crosses between the white- and red-fruited genotypes. Asexual multiplications were carried out by stolon rooting and sexual multiplications by germination of achenes obtained from crosses. The expression level of the genes involved in the synthesis and regulation of the anthocyanins pathway (CHS, F3H, DFR, ANS, and MYB10) were evaluated by RT-PCR using specific primers. Key results: Plants with the white-fruited character always yielded white-fruited progeny when propagated asexually, whereas in sexually propagated plants fruit color depended on the mother. Red-fruited mothers yielded red-fruited progeny, and whitefruited mothers yielded fruits ranging from dark pink to white. Molecular analysis suggested that the white-fruited character was due to the low expression of the ANS gene. Conclusions: Results obtained indicate that the white-fruited character was stable. Mother progenitors exert a strong influence on the expression of the white-fruited character. The white-fruited phenotype is due to the impairment or downregulation of the ANS gene. © 2011 Botanical Society of America.
author	Wulff, Arturo Federico
author_facet	Wulff, Arturo Federico
author_sort	Wulff, Arturo Federico
title	White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
title_short	White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
title_full	White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
title_fullStr	White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
title_full_unstemmed	White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression
title_sort	white-fruited duchesnea indica (rosaceae) is impaired in ans gene expression
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029122_v98_n12_p2077_Debes http://hdl.handle.net/20.500.12110/paper_00029122_v98_n12_p2077_Debes
work_keys_str_mv	AT wulffarturofederico whitefruitedduchesneaindicarosaceaeisimpairedinansgeneexpression
_version_	1768545167502999552

White-fruited duchesnea indica (Rosaceae) is impaired in ANS gene expression

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