Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregatio...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v132_n23_p7860_Yushchenko http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko |
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paper:paper_00027863_v132_n23_p7860_Yushchenko2023-06-08T14:22:47Z Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation Fauerbach, Jonathan Arturo Jares, Elizabeth Andrea Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease. © 2010 American Chemical Society. Fil:Fauerbach, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v132_n23_p7860_Yushchenko http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors |
| spellingShingle |
Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors Fauerbach, Jonathan Arturo Jares, Elizabeth Andrea Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation |
| topic_facet |
Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors |
| description |
We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease. © 2010 American Chemical Society. |
| author |
Fauerbach, Jonathan Arturo Jares, Elizabeth Andrea |
| author_facet |
Fauerbach, Jonathan Arturo Jares, Elizabeth Andrea |
| author_sort |
Fauerbach, Jonathan Arturo |
| title |
Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation |
| title_short |
Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation |
| title_full |
Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation |
| title_fullStr |
Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation |
| title_full_unstemmed |
Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation |
| title_sort |
fluorescent ratiometric mfc probe sensitive to early stages of α-synuclein aggregation |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v132_n23_p7860_Yushchenko http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko |
| work_keys_str_mv |
AT fauerbachjonathanarturo fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation AT jareselizabethandrea fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation |
| _version_ |
1768541776195354624 |