Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins

We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensi...

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Detalles Bibliográficos
Autores principales: Spagnuolo, Carla Cecilia, Jares, Elizabeth Andrea
Publicado: 2006
Materias:
arsenic derivative
biotin
cysteine derivative
dye
fluorescein
absorption spectroscopy
anisotropy
article
chemical structure
complex formation
fluorescence
fluorescence resonance energy transfer
fluorescence spectroscopy
fluorination
irradiation
pH
photochemistry
Arsenicals
Cysteine
Drug Stability
Fluoresceins
Fluorescence Polarization
Fluorescence Resonance Energy Transfer
Hydrocarbons, Fluorinated
Hydrogen-Ion Concentration
Oligopeptides
Photochemistry
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v128_n37_p12040_Spagnuolo
http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes. Copyright © 2006 American Chemical Society.

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