Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins

We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensi...

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Autores principales: Spagnuolo, Carla Cecilia, Jares, Elizabeth Andrea
Publicado: 2006
Materias:
arsenic derivative
biotin
cysteine derivative
dye
fluorescein
absorption spectroscopy
anisotropy
article
chemical structure
complex formation
fluorescence
fluorescence resonance energy transfer
fluorescence spectroscopy
fluorination
irradiation
pH
photochemistry
Arsenicals
Cysteine
Drug Stability
Fluoresceins
Fluorescence Polarization
Fluorescence Resonance Energy Transfer
Hydrocarbons, Fluorinated
Hydrogen-Ion Concentration
Oligopeptides
Photochemistry
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v128_n37_p12040_Spagnuolo
http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00027863_v128_n37_p12040_Spagnuolo
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spelling paper:paper_00027863_v128_n37_p12040_Spagnuolo2023-06-08T14:22:41Z Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins Spagnuolo, Carla Cecilia Jares, Elizabeth Andrea arsenic derivative biotin cysteine derivative dye fluorescein absorption spectroscopy anisotropy article chemical structure complex formation fluorescence fluorescence resonance energy transfer fluorescence spectroscopy fluorination irradiation pH photochemistry Arsenicals Cysteine Drug Stability Fluoresceins Fluorescence Polarization Fluorescence Resonance Energy Transfer Hydrocarbons, Fluorinated Hydrogen-Ion Concentration Oligopeptides Photochemistry We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes. Copyright © 2006 American Chemical Society. Fil:Spagnuolo, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v128_n37_p12040_Spagnuolo http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic arsenic derivative
biotin
cysteine derivative
dye
fluorescein
absorption spectroscopy
anisotropy
article
chemical structure
complex formation
fluorescence
fluorescence resonance energy transfer
fluorescence spectroscopy
fluorination
irradiation
pH
photochemistry
Arsenicals
Cysteine
Drug Stability
Fluoresceins
Fluorescence Polarization
Fluorescence Resonance Energy Transfer
Hydrocarbons, Fluorinated
Hydrogen-Ion Concentration
Oligopeptides
Photochemistry
spellingShingle arsenic derivative
biotin
cysteine derivative
dye
fluorescein
absorption spectroscopy
anisotropy
article
chemical structure
complex formation
fluorescence
fluorescence resonance energy transfer
fluorescence spectroscopy
fluorination
irradiation
pH
photochemistry
Arsenicals
Cysteine
Drug Stability
Fluoresceins
Fluorescence Polarization
Fluorescence Resonance Energy Transfer
Hydrocarbons, Fluorinated
Hydrogen-Ion Concentration
Oligopeptides
Photochemistry
Spagnuolo, Carla Cecilia
Jares, Elizabeth Andrea
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
topic_facet arsenic derivative
biotin
cysteine derivative
dye
fluorescein
absorption spectroscopy
anisotropy
article
chemical structure
complex formation
fluorescence
fluorescence resonance energy transfer
fluorescence spectroscopy
fluorination
irradiation
pH
photochemistry
Arsenicals
Cysteine
Drug Stability
Fluoresceins
Fluorescence Polarization
Fluorescence Resonance Energy Transfer
Hydrocarbons, Fluorinated
Hydrogen-Ion Concentration
Oligopeptides
Photochemistry
description We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes. Copyright © 2006 American Chemical Society.
author Spagnuolo, Carla Cecilia
Jares, Elizabeth Andrea
author_facet Spagnuolo, Carla Cecilia
Jares, Elizabeth Andrea
author_sort Spagnuolo, Carla Cecilia
title Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
title_short Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
title_full Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
title_fullStr Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
title_full_unstemmed Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
title_sort improved photostable fret-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
publishDate 2006
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v128_n37_p12040_Spagnuolo
http://hdl.handle.net/20.500.12110/paper_00027863_v128_n37_p12040_Spagnuolo
work_keys_str_mv AT spagnuolocarlacecilia improvedphotostablefretcompetentbiarsenicaltetracysteineprobesbasedonfluorinatedfluoresceins
AT jareselizabethandrea improvedphotostablefretcompetentbiarsenicaltetracysteineprobesbasedonfluorinatedfluoresceins
_version_ 1768544664080613376

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