The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata
Fil: Milani, Diogo. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Biología; Brasil.
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
BioMed Central
2017
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| Acceso en línea: | https://hdl.handle.net/20.500.12219/4414 |
| Aporte de: |
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I77-R195-20.500.12219-4414 |
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| record_format |
dspace |
| institution |
Universidad Nacional de Misiones |
| institution_str |
I-77 |
| repository_str |
R-195 |
| collection |
Repositorio Institucional Digital de la UNaM (RIDUNAM) |
| language |
Inglés |
| topic |
B chromosome Repetitive DNA Tandem repeat Transcription |
| spellingShingle |
B chromosome Repetitive DNA Tandem repeat Transcription Milani, Diogo Ramos, Érica Loreto, Vilma Martí, Dardo Andrea Lima Cardoso, Adauto Martínez de Moraes, Karen Cristiane Martins, César Cabral de Mello, Diogo Cavalcanti The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata |
| topic_facet |
B chromosome Repetitive DNA Tandem repeat Transcription |
| description |
Fil: Milani, Diogo. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Biología; Brasil. |
| format |
Artículo Artículo publishedVersion |
| author |
Milani, Diogo Ramos, Érica Loreto, Vilma Martí, Dardo Andrea Lima Cardoso, Adauto Martínez de Moraes, Karen Cristiane Martins, César Cabral de Mello, Diogo Cavalcanti |
| author_facet |
Milani, Diogo Ramos, Érica Loreto, Vilma Martí, Dardo Andrea Lima Cardoso, Adauto Martínez de Moraes, Karen Cristiane Martins, César Cabral de Mello, Diogo Cavalcanti |
| author_sort |
Milani, Diogo |
| title |
The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata |
| title_short |
The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata |
| title_full |
The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata |
| title_fullStr |
The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata |
| title_full_unstemmed |
The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata |
| title_sort |
satellite dna aflasat-1 in the a and b chromosomes of the grasshopper abracris flavolineata |
| publisher |
BioMed Central |
| publishDate |
2017 |
| url |
https://hdl.handle.net/20.500.12219/4414 |
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I77-R195-20.500.12219-44142024-06-04T10:40:09Z The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata Milani, Diogo Ramos, Érica Loreto, Vilma Martí, Dardo Andrea Lima Cardoso, Adauto Martínez de Moraes, Karen Cristiane Martins, César Cabral de Mello, Diogo Cavalcanti B chromosome Repetitive DNA Tandem repeat Transcription Fil: Milani, Diogo. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Biología; Brasil. Fil: Ramos, Érica. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Morfología; Brasil. Fil: Loreto, Vilma. Universidad Federal de Pernambuco. Centro de Biociencias. Departamento de Genética; Brasil. Fil: Martí, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina. Fil: Martí, Dardo Andrea. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Química y Naturales. Instituto de Biología Subtropical; Argentina. Fil: Lima Cardoso, Adauto. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Morfología; Brasil. Fil: Martínez de Moraes, Karen Cristiane. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Biología; Brasil. Fil: Martins, César. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Morfología; Brasil. Fil: Cabral de Mello, Diogo Cavalcanti. Universidad Estatal Paulista Julio de Mesquita Filho. Instituto de Biociencias. Departamento de Biología; Brasil. Background: Satellite DNAs (satDNAs) are organized in repetitions directly contiguous to one another, forming long arrays and composing a large portion of eukaryote genomes. These sequences evolve according to the concerted evolution model, and homogenization of repeats is observed at the intragenomic level. Satellite DNAs are the primary component of heterochromatin, located primarily in centromeres and telomeres. Moreover, satDNA enrichment in specific chromosomes has been observed, such as in B chromosomes, that can provide clues about composition, origin and evolution of this chromosome. In this study, we isolated and characterized a satDNA in A and B chromosomes of Abracris flavolineata by integrating cytogenetic, molecular and genomics approaches at intra- and inter-population levels, with the aim to understand the evolution of satDNA and composition of B chromosomes. Results: AflaSAT-1 satDNA was shared with other species and in A. flavolineata, was associated with another satDNA, AflaSAT-2. Chromosomal mapping revealed centromeric blocks variable in size in almost all chromosomes (except pair 11) of A complement for both satDNAs, whereas for B chromosome, only a small centromeric signal occurred. In distinct populations, variable number of AflaSAT-1 chromosomal sites correlated with variability in copy number. Instead of such variability, low sequence diversity was observed in A complement, but monomers from B chromosome were more variable, presenting also exclusive mutations. AflaSAT-1 was transcribed in five tissues of adults in distinct life cycle phases. Conclusions: The sharing of AflaSAT-1 with other species is consistent with the library hypothesis and indicates common origin in a common ancestor; however, AflaSAT-1 was highly amplified in the genome of A. flavolineata. At the population level, homogenization of repeats in distinct populations was documented, but dynamic expansion or elimination of repeats was also observed. Concerning the B chromosome, our data provided new information on the composition in A. flavolineata. Together with previous results, the sequences of heterochromatic nature were not likely highly amplified in the entire B chromosome. Finally, the constitutive transcriptional activity suggests a possible unknown functional role, which should be further investigated. 2017-08-29 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12219/4414 eng info:eu-repo/semantics/altIdentifier/urn/https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-017-0548-9 info:eu-repo/semantics/openAccess application/pdf application/pdf 3.543 MB BioMed Central |