Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina

Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Microbiología. Cátedra de Bacteriología; Argentina.

Detalles Bibliográficos
Autores principales: Novosak, Marina Gisel, Bobadilla, Fernando Javier, Delgado, Osvaldo Daniel, Vergara, Marta, Laczeski, Margarita Ester
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Mary Ann Liebert 2020
Materias:
Streptococcus agalactiae
Antibiotic resistance
E-test
D-test
RAPD
Acceso en línea:https://hdl.handle.net/20.500.12219/3335
Aporte de:
Repositorio Institucional Digital de la UNaM (RIDUNAM) de Universidad Nacional de Misiones
id I77-R195-20.500.12219-3335
record_format dspace
institution Universidad Nacional de Misiones
institution_str I-77
repository_str R-195
collection Repositorio Institucional Digital de la UNaM (RIDUNAM)
language Inglés
topic Streptococcus agalactiae
Antibiotic resistance
E-test
D-test
RAPD
spellingShingle Streptococcus agalactiae
Antibiotic resistance
E-test
D-test
RAPD
Novosak, Marina Gisel
Bobadilla, Fernando Javier
Delgado, Osvaldo Daniel
Vergara, Marta
Laczeski, Margarita Ester
Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina
topic_facet Streptococcus agalactiae
Antibiotic resistance
E-test
D-test
RAPD
description Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Microbiología. Cátedra de Bacteriología; Argentina.
format Artículo
Artículo
publishedVersion
author Novosak, Marina Gisel
Bobadilla, Fernando Javier
Delgado, Osvaldo Daniel
Vergara, Marta
Laczeski, Margarita Ester
author_facet Novosak, Marina Gisel
Bobadilla, Fernando Javier
Delgado, Osvaldo Daniel
Vergara, Marta
Laczeski, Margarita Ester
author_sort Novosak, Marina Gisel
title Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina
title_short Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina
title_full Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina
title_fullStr Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina
title_full_unstemmed Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina
title_sort phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in misiones, argentina
publisher Mary Ann Liebert
publishDate 2020
url https://hdl.handle.net/20.500.12219/3335
work_keys_str_mv AT novosakmarinagisel phenotypicandgenotypiccharacterizationofresistancetomacrolidesandlincosamidesinstreptococcusagalactiaeisolatedfrompregnantwomeninmisionesargentina
AT bobadillafernandojavier phenotypicandgenotypiccharacterizationofresistancetomacrolidesandlincosamidesinstreptococcusagalactiaeisolatedfrompregnantwomeninmisionesargentina
AT delgadoosvaldodaniel phenotypicandgenotypiccharacterizationofresistancetomacrolidesandlincosamidesinstreptococcusagalactiaeisolatedfrompregnantwomeninmisionesargentina
AT vergaramarta phenotypicandgenotypiccharacterizationofresistancetomacrolidesandlincosamidesinstreptococcusagalactiaeisolatedfrompregnantwomeninmisionesargentina
AT laczeskimargaritaester phenotypicandgenotypiccharacterizationofresistancetomacrolidesandlincosamidesinstreptococcusagalactiaeisolatedfrompregnantwomeninmisionesargentina
_version_ 1809776636721102848
spelling I77-R195-20.500.12219-33352024-05-31T13:50:37Z Phenotypic and genotypic characterization of resistance to macrolides and lincosamides in streptococcus agalactiae isolated from pregnant women in Misiones, Argentina Novosak, Marina Gisel Bobadilla, Fernando Javier Delgado, Osvaldo Daniel Vergara, Marta Laczeski, Margarita Ester Streptococcus agalactiae Antibiotic resistance E-test D-test RAPD Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Microbiología. Cátedra de Bacteriología; Argentina. Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Microbiología. Cátedra de Bacteriología; Argentina. Fil: Bobadilla, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Delgado, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Fil: Delgado, Osvaldo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Centro de Biología Molecular y Biotecnología; Argentina. Fil: Vergara, Marta. Marina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Microbiología. Cátedra de Bacteriología; Argentina. Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Microbiología. Cátedra de Bacteriología; Argentina. Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Biotecnología Misiones ‘‘Dra. María Ebe Reca’’. Laboratorio de Biotecnología Molecular; Argentina. The aim of this work was to determine the susceptibility, molecular profile, and clonal relationship in Strep tococcus agalactiae (group B Streptococcus [GBS]) isolated from vaginal-rectal swab samples. We worked with 200 isolates collected from pregnant women between 35 and 37 weeks of gestation. The macrolide– lincosamide–streptogramin BMLSB) resistance phenotypes were determined using the double-disc assay. Susceptibility to erythromycin (ERI) and clindamycin (CLI) was performed with the E-test. Resistance genes ermB and ermTR were detected by polymerase chain reaction. Clonal studies were performed using the random amplification of polymorphic DNA. Twelve (6%) of the isolates were resistant to ERI and 10 (5%) of them to CLI. Fifty percent of the resistant strains corresponded to serotype III, 25% to serotype V, and the remaining 25% to serotype Ia, II, and nontypeable strains. The Cmlsb phenotype was detected in eight strains (66.67%) and the iMLSB phenotype in four (33.33%). The minimum inhibitory concentration values were between 1.5 mg mg/ and 16 /mL for ERI, and between 1 and 32 mL for CLI. Out of the 25 strains susceptible to ERI and CLI, the presence of the ermB gene was detected in eight of them and the ermTR gene in one strain. The ermB gene was detected in the 12 strains that initially had some macrolide resistance phenotype. The ermTR gene was detected in three out of the four strains with the iMLSB phenotype. The resistance to macrolides in the province of Misiones is due to multiclonal spread. The phenotypic and genotypic characterization of macrolide resistance in GBS strains are crucial to contribute to the correct intrapartum prophylactic antibiotic therapy of allergic pregnant women and the epidemiological surveillance of these strains. 2020-12-03 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12219/3335 eng info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1089/mdr.2019.0328 info:eu-repo/semantics/openAccess application/pdf application/pdf 303 KB Mary Ann Liebert

Ejemplares similares