Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.

The bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce bio...

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Detalles Bibliográficos
Autores principales: Conde Molina, Débora, Liporace, Franco, Vázquez, Susana, Merini, Luciano, Quevedo, Carla
Formato: Documento de conferencia acceptedVersion
Lenguaje:Español
Publicado: Argentine Society for Biochemistry and Molecular Biology 2022
Materias:
UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
Acceso en línea:http://hdl.handle.net/20.500.12272/6168
Aporte de:
RIA - Repositorio Institucional Abierto (UTN) de Universidad Tecnológica Nacional
id I68-R174-20.500.12272-6168
record_format dspace
institution Universidad Tecnológica Nacional
institution_str I-68
repository_str R-174
collection RIA - Repositorio Institucional Abierto (UTN)
language Español
topic UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
spellingShingle UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
Conde Molina, Débora
Liporace, Franco
Vázquez, Susana
Merini, Luciano
Quevedo, Carla
Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
topic_facet UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
description The bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce biosurfactants was evaluated in Erlenmeyer flasks containing a minimal saline medium (MSM) supplemented with different carbon sources: a mixture of hydrocarbons (HC; 4.5%v/v), glycerol (Gly; 2%v/v), sunflower oil (SO; 2%v/v) and peanut oil (PO; 2%v/v). Cultures were performed at 135 rpm and 25 ºC for 4 days. Bacterial growth was measured by cell dry-weight method, and biosurfactant production was estimated by direct measurement of the surface tension (ST). Results showed that bacteria was able to grow on all the carbon sources tested, reaching concentrations of 1.24g/l in HC, 7.69g/l in Gly, 3.98g/l in SO and 9.29g/l in PO. Culture supernatants showed a decrease in ST values when the strain grew on SO and PO (22.5% and 25.5%). No decrease in ST values was observed when HC and Gly were used as carbon source. As results shown, this bacterial strain can produce biosurfactants under certain culture conditions. This potential advantage could be applied in bioremediation strategy of hydrocarbon contaminated sites.
format Documento de conferencia
acceptedVersion
author Conde Molina, Débora
Liporace, Franco
Vázquez, Susana
Merini, Luciano
Quevedo, Carla
author_facet Conde Molina, Débora
Liporace, Franco
Vázquez, Susana
Merini, Luciano
Quevedo, Carla
author_sort Conde Molina, Débora
title Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_short Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_full Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_fullStr Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_full_unstemmed Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_sort bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
publisher Argentine Society for Biochemistry and Molecular Biology
publishDate 2022
url http://hdl.handle.net/20.500.12272/6168
work_keys_str_mv AT condemolinadebora bioremediationstrategiesbasedonanativestrainisolatedfromsitescontaminatedwithhydrocarbons
AT liporacefranco bioremediationstrategiesbasedonanativestrainisolatedfromsitescontaminatedwithhydrocarbons
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AT meriniluciano bioremediationstrategiesbasedonanativestrainisolatedfromsitescontaminatedwithhydrocarbons
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