Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner

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ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Infectious bursal disease virus (IBDV) internalization is sparsely known in terms of molecular components of the pathway involved. To describe the cell biological features of IBDV endocytosis, we employed perturbants of endocytic pathways such as pharmacol...

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Autores principales: Gimenez, María Cecilia, Rodríguez Aguirre, José Francisco, Colombo, María Isabel, Delgui, Laura Ruth
Formato: Artículo Científico
Lenguaje:Inglés
Publicado: Comité editorial Cellular Microbiology 2020
Acceso en línea:http://repositorio.umaza.edu.ar//handle/00261/1816
https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12415
Aporte de:
UMAZA Digital (Universidad MAZA - Mendoza) de Universidad Juan Agustín MAZA
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institution Universidad Juan Agustín MAZA
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collection UMAZA Digital (Universidad MAZA - Mendoza)
language Inglés
orig_language_str_mv eng
description ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Infectious bursal disease virus (IBDV) internalization is sparsely known in terms of molecular components of the pathway involved. To describe the cell biological features of IBDV endocytosis, we employed perturbants of endocytic pathways such as pharmacological inhibitors and overexpression of dominant-negative mutants. Internalization analysis was performed quantifying infected cells by immunofluorescence and Western blot detection of the viral protein VP3 at 12 h post-infection reinforced by the analysis of the capsid protein VP2 localization after virus uptake at 1 h post-infection. We compared IBDV infection to the internalization of well-established ligands with defined endocytic pathways: transferrin, cholera-toxin subunit B and dextran. To describe virus endocytosis at the morphological level, we performed ultrastructural studies of viral internalization kinetics in control and actin dynamics-blocked cells. Our results indicate that IBDV endocytic internalization was clathrin- and dynamin-independent, and that IBDV uses macropinocytosis as the primary entry mechanism. After uptake, virus traffics to early endosomes and requires exposure to the low endocytic pH as well as a functional endocytic pathway to complete its replication cycle. Moreover, our results indicate that the GTPase Rab5 is crucial for IBDV entry supporting the participation of the early endosomal pathway in IBDV internalization and infection of susceptible cells. Sitio de la revista: https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12415
format Artículo Científico
author Gimenez, María Cecilia
Rodríguez Aguirre, José Francisco
Colombo, María Isabel
Delgui, Laura Ruth
spellingShingle Gimenez, María Cecilia
Rodríguez Aguirre, José Francisco
Colombo, María Isabel
Delgui, Laura Ruth
Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
author_facet Gimenez, María Cecilia
Rodríguez Aguirre, José Francisco
Colombo, María Isabel
Delgui, Laura Ruth
author_sort Gimenez, María Cecilia
title Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
title_short Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
title_full Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
title_fullStr Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
title_full_unstemmed Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
title_sort infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a rab5-dependent manner
publisher Comité editorial Cellular Microbiology
publishDate 2020
url http://repositorio.umaza.edu.ar//handle/00261/1816
https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12415
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AT colombomariaisabel infectiousbursaldiseasevirusuptakeinvolvesmacropinocytosisandtraffickingtoearlyendosomesinarab5dependentmanner
AT delguilauraruth infectiousbursaldiseasevirusuptakeinvolvesmacropinocytosisandtraffickingtoearlyendosomesinarab5dependentmanner
bdutipo_str Repositorios
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