Pseudobutyrivibrio xylanivorans adhesion to epithelial cells

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The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the major requirements for probiotics. In this...

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Autores principales: Grilli, Diego Javier, Mansilla, María Eugenia, Gimenez, María Cecilia, Sohaefer, Noelia, Ruiz, María Soledad, Terebiznik, Mauricio, Sosa, Miguel, Arenas, Graciela Nora
Formato: Institucional - Material de Difusión
Lenguaje:Inglés
Publicado: Comité editorial Anaerobe Journal 2020
Materias:
Rumen
Anaerobic bacteria
Probiotic
Adhesion
Acceso en línea:http://repositorio.umaza.edu.ar//handle/00261/1822
https://www.sciencedirect.com/science/article/abs/pii/S1075996419300010#!
Aporte de:
UMAZA Digital (Universidad MAZA - Mendoza) de Universidad Juan Agustín MAZA
id I56-R162-00261-1822
record_format dspace
spelling I56-R162-00261-18222023-08-19T17:58:10Z Pseudobutyrivibrio xylanivorans adhesion to epithelial cells Grilli, Diego Javier Mansilla, María Eugenia Gimenez, María Cecilia Sohaefer, Noelia Ruiz, María Soledad Terebiznik, Mauricio Sosa, Miguel Arenas, Graciela Nora Rumen Anaerobic bacteria Probiotic Adhesion The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the major requirements for probiotics. In this study, we assessed the adhesion of P. xylanivorans 2 to SW480 cells and characterized this process utilizing multiple microscopy approaches. Our results indicate that a multiplicity of infection of 200 CFU/cell allows the highest bacteria to cell binding ratio, with a lower percentage of auto-agglutination events. The comparison of the adherence capacity subjected heat-shock treatment (100 C, 1 min), which produces the denaturalization of proteins at the bacterial surface, as opposed untreated P. xylanivorans, suggested that this bacteria may attach to SW480 cells utilizing a proteinaceous structure. Confocal microscopy analyses indicate that P. xylanivorans 2 attachment induces the formation of F-actin-enriched areas on the surface of SW480 cells. Transmission electron microscopy (TEM) revealed the formation of a structure similar to a pedestal in the area of the epithelial cell surface, where the bacterium rests. Finally, a casual finding of TEM analysis of transverse and longitudinal thin-sections of P. xylanivorans 2, revealed irregular intracytoplasmic structures compatibles with the so-called bacterial microcompartments. This is the first ultrastructural description of bacterial microcompartments-like structures in the genus Pseudobutyrivibrio. 2020-11-07T09:16:26Z 2020-11-07T09:16:26Z 2019-01-05 Institucional - Material de Difusión Grilli, D. J., Mansill, M. E., Giménez, M. C., Sohaefer, N., Ruiz, M. S., Terebiznik, M., Sosa, M., y Arenas, G. N. (2019) Pseudobutyrivibrio xylanivorans adhesion to epithelial cells.Anaerobe Society of the Americas , 56, 1-7. https://doi.org/10.1016/j.anaerobe.2019.01.001 1075-9964 http://repositorio.umaza.edu.ar//handle/00261/1822 https://www.sciencedirect.com/science/article/abs/pii/S1075996419300010#! eng image/png Comité editorial Anaerobe Journal 56;
institution Universidad Juan Agustín MAZA
institution_str I-56
repository_str R-162
collection UMAZA Digital (Universidad MAZA - Mendoza)
language Inglés
orig_language_str_mv eng
topic Rumen
Anaerobic bacteria
Probiotic
Adhesion
spellingShingle Rumen
Anaerobic bacteria
Probiotic
Adhesion
Grilli, Diego Javier
Mansilla, María Eugenia
Gimenez, María Cecilia
Sohaefer, Noelia
Ruiz, María Soledad
Terebiznik, Mauricio
Sosa, Miguel
Arenas, Graciela Nora
Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
topic_facet Rumen
Anaerobic bacteria
Probiotic
Adhesion
description The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the major requirements for probiotics. In this study, we assessed the adhesion of P. xylanivorans 2 to SW480 cells and characterized this process utilizing multiple microscopy approaches. Our results indicate that a multiplicity of infection of 200 CFU/cell allows the highest bacteria to cell binding ratio, with a lower percentage of auto-agglutination events. The comparison of the adherence capacity subjected heat-shock treatment (100 C, 1 min), which produces the denaturalization of proteins at the bacterial surface, as opposed untreated P. xylanivorans, suggested that this bacteria may attach to SW480 cells utilizing a proteinaceous structure. Confocal microscopy analyses indicate that P. xylanivorans 2 attachment induces the formation of F-actin-enriched areas on the surface of SW480 cells. Transmission electron microscopy (TEM) revealed the formation of a structure similar to a pedestal in the area of the epithelial cell surface, where the bacterium rests. Finally, a casual finding of TEM analysis of transverse and longitudinal thin-sections of P. xylanivorans 2, revealed irregular intracytoplasmic structures compatibles with the so-called bacterial microcompartments. This is the first ultrastructural description of bacterial microcompartments-like structures in the genus Pseudobutyrivibrio.
format Institucional - Material de Difusión
author Grilli, Diego Javier
Mansilla, María Eugenia
Gimenez, María Cecilia
Sohaefer, Noelia
Ruiz, María Soledad
Terebiznik, Mauricio
Sosa, Miguel
Arenas, Graciela Nora
author_facet Grilli, Diego Javier
Mansilla, María Eugenia
Gimenez, María Cecilia
Sohaefer, Noelia
Ruiz, María Soledad
Terebiznik, Mauricio
Sosa, Miguel
Arenas, Graciela Nora
author_sort Grilli, Diego Javier
title Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
title_short Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
title_full Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
title_fullStr Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
title_full_unstemmed Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
title_sort pseudobutyrivibrio xylanivorans adhesion to epithelial cells
publisher Comité editorial Anaerobe Journal
publishDate 2020
url http://repositorio.umaza.edu.ar//handle/00261/1822
https://www.sciencedirect.com/science/article/abs/pii/S1075996419300010#!
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