Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation

Pollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at 4,L20,L70, andL196 8C. Fertilizing ability of...

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Detalles Bibliográficos
Autores principales: Dolce, Natalia Raquel, Medina, Ricardo Daniel, Mroginski, Luis Amado, Rey, Hebe Yolanda
Formato: Artículo
Lenguaje:Inglés
Publicado: American Society for Horticultural Science 2020
Materias:
Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9170
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-9170
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
spellingShingle Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
topic_facet Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
description Pollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at 4,L20,L70, andL196 8C. Fertilizing ability of fresh and stored (30 to 360 days) pollinia was determined by the fruit and seed formation for each treatment, as well as by the seed viability, in vitro seed germination, and seedling growth. Pollinia stored at L70 and L196 8C showed high fertilizing ability (94.4% to 100.0%) even 1 year after collection, revealing no significant differences with fresh pollinia. Seeds from all treatments showed high viability (91.2% to 94.3%) through the 2,3,5-triphenyltetrazolium chloride (TTC) reduction assay and high in vitro germination (91.7% to 97.3%). Thus, successful ultracold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection, or precooling treatment before placing into an ultra freezer (L70 8C) or immersing in liquid nitrogen (LN) (L196 8C).
format Artículo
author Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_facet Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_sort Dolce, Natalia Raquel
title Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_short Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_full Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_fullStr Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_full_unstemmed Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_sort storage of cohniella cepula (orchidaceae) pollinia : fertilizing ability and subsequent fruit and seed formation
publisher American Society for Horticultural Science
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9170
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AT medinaricardodaniel storageofcohniellacepulaorchidaceaepolliniafertilizingabilityandsubsequentfruitandseedformation
AT mroginskiluisamado storageofcohniellacepulaorchidaceaepolliniafertilizingabilityandsubsequentfruitandseedformation
AT reyhebeyolanda storageofcohniellacepulaorchidaceaepolliniafertilizingabilityandsubsequentfruitandseedformation
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