Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation

The species of the genus Arachis (Leguminosae) are ordered into nine sections. The assignment of genome types in this genus has been based on cross-compatibility analysis and molecular cytogenetic studies. The latter has also allowed karyotypically establishing well-defined genomes and reassigning t...

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Detalles Bibliográficos
Autores principales: Silvestri, María Celeste, Ortiz, Alejandra Marcela, Robledo Dobladez, Germán Ariel, Lavia, Graciela Inés
Formato: Artículo
Lenguaje:Inglés
Publicado: Academia Brasileira de Ciencias 2023
Materias:
Heterochromatin patterns
Karyotype groups
Molecular cytogenetic
RDNA­loci
SatDNA
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/52948
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-52948
record_format dspace
spelling I48-R184-123456789-529482023-11-21T15:27:17Z Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation Silvestri, María Celeste Ortiz, Alejandra Marcela Robledo Dobladez, Germán Ariel Lavia, Graciela Inés Heterochromatin patterns Karyotype groups Molecular cytogenetic RDNA­loci SatDNA The species of the genus Arachis (Leguminosae) are ordered into nine sections. The assignment of genome types in this genus has been based on cross-compatibility analysis and molecular cytogenetic studies. The latter has also allowed karyotypically establishing well-defined genomes and reassigning the genome of several species. However, most of these studies have been focused mainly on the sections Arachis and Rhizomatosae. To increase the knowledge about the chromosome diversity of the whole genus, here we performed a detailed karyotype characterization of representative species of most of the sections and genomes of Arachis. This characterization included chromosome morphology, CMA/DAPI chromosome banding, and chromosome marker localization (rDNAloci and one satDNA sequence) by fluorescent in situ hybridization (FISH). Based on the data obtained and other previously published data, we established the karyotype similarities by cluster analysis and defined eleven karyotype groups. The grouping was partly coincident with the traditional genome assignment, except for some groups and some individual species. Karyotype similarities among some genomes were also found. The main characteristics of each karyotype group of Arachis were summarized. Together, our results provide information that may be beneficial for future cytogenetic and evolutionary studies, and also contribute to the identification of interspecific hybrids. 2023-11-15T11:16:15Z 2023-11-15T11:16:15Z 2020 Artículo Silvestri, María Celeste, et al., 2020. Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation. Anais da Academia Brasileira de Ciencias. Rio de Janeiro: Academia Brasileira de Ciencias, vol. 92, supl. 2, p. 1-29. E-ISSN 1678-2690. 0001-3765 http://repositorio.unne.edu.ar/handle/123456789/52948 eng https://www.scielo.br/j/aabc/a/8zTPpjkBHkntBZMFhXbXDKr/?format=pdf&lang=en openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-29 application/pdf Academia Brasileira de Ciencias Anais da Academia Brasileira de Ciencias, 2020, vol. 92, supl. 2, p. 1-29.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Heterochromatin patterns
Karyotype groups
Molecular cytogenetic
RDNA­loci
SatDNA
spellingShingle Heterochromatin patterns
Karyotype groups
Molecular cytogenetic
RDNA­loci
SatDNA
Silvestri, María Celeste
Ortiz, Alejandra Marcela
Robledo Dobladez, Germán Ariel
Lavia, Graciela Inés
Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation
topic_facet Heterochromatin patterns
Karyotype groups
Molecular cytogenetic
RDNA­loci
SatDNA
description The species of the genus Arachis (Leguminosae) are ordered into nine sections. The assignment of genome types in this genus has been based on cross-compatibility analysis and molecular cytogenetic studies. The latter has also allowed karyotypically establishing well-defined genomes and reassigning the genome of several species. However, most of these studies have been focused mainly on the sections Arachis and Rhizomatosae. To increase the knowledge about the chromosome diversity of the whole genus, here we performed a detailed karyotype characterization of representative species of most of the sections and genomes of Arachis. This characterization included chromosome morphology, CMA/DAPI chromosome banding, and chromosome marker localization (rDNAloci and one satDNA sequence) by fluorescent in situ hybridization (FISH). Based on the data obtained and other previously published data, we established the karyotype similarities by cluster analysis and defined eleven karyotype groups. The grouping was partly coincident with the traditional genome assignment, except for some groups and some individual species. Karyotype similarities among some genomes were also found. The main characteristics of each karyotype group of Arachis were summarized. Together, our results provide information that may be beneficial for future cytogenetic and evolutionary studies, and also contribute to the identification of interspecific hybrids.
format Artículo
author Silvestri, María Celeste
Ortiz, Alejandra Marcela
Robledo Dobladez, Germán Ariel
Lavia, Graciela Inés
author_facet Silvestri, María Celeste
Ortiz, Alejandra Marcela
Robledo Dobladez, Germán Ariel
Lavia, Graciela Inés
author_sort Silvestri, María Celeste
title Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation
title_short Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation
title_full Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation
title_fullStr Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation
title_full_unstemmed Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/ DAPI banding, and inferences about their karyotype differentiation
title_sort chromosome diversity in species of the genus arachis, revealed by fish and cma/ dapi banding, and inferences about their karyotype differentiation
publisher Academia Brasileira de Ciencias
publishDate 2023
url http://repositorio.unne.edu.ar/handle/123456789/52948
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