Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom

Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We h...

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Autores principales: Rodríguez, Juan Pablo, De Marzi, Mauricio César, Maruñak, Silvana Licia, Malchiodi, Emilio Luis, Leiva, Laura Cristina Ana, Acosta, Ofelia Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: Fundación Revista Medicina 2023
Materias:
Crotalus durissus terrificus
Phospholipase A2
Antibodies IgG
Neutralization
Fosfolipasa A2
Anticuerpos IgG
Neutralización
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/51204
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-51204
record_format dspace
spelling I48-R184-123456789-512042025-03-06T11:00:36Z Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom Rodríguez, Juan Pablo De Marzi, Mauricio César Maruñak, Silvana Licia Malchiodi, Emilio Luis Leiva, Laura Cristina Ana Acosta, Ofelia Cristina Crotalus durissus terrificus Phospholipase A2 Antibodies IgG Neutralization Fosfolipasa A2 Anticuerpos IgG Neutralización Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75). Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 μg) with Freund adjuvant. Groups of six mice (20 + 2 g) were inoculated with 0.5 ml i.p. of C. d. t. venom (4 μg) or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms. Los anticuerpos IgG de conejos anti-fosfolipasa A2 de Crotalus durissus terrificus neutralizan la actividad letal del veneno. El veneno de Crotalus durissus terrificus (C.d.t.) (Cascabel de Sud América) posee actividad miotóxica y neurotóxica, actividades que también exhibe el complejo crotoxina, princi- pal componente tóxico de este veneno. El complejo crotoxina está constituido por una fosfolipasa A2 básica (PLA2 ) y una proteína acídica no tóxica, el crotapotín. En este trabajo se estudió la capacidad neutralizante de anticuerpos IgG anti-PLA2 sobre la letalidad inducida por el veneno entero. El antígeno PLA2, fue aislado por cromatografía de filtración en gel (Sephadex G-75). Se inocularon conejos machos por vía subcutánea e intra- muscular, con 700 μg de PLA2 y adyuvante para la obtención de anticuerpos específicos. La capacidad neutra- lizante del antisuero se analizó en ratones por inoculación con diluciones de veneno entero preincubado con un volumen adecuado de anticuerpos IgG anti-PLA2. Se inocularion ratones controles con 0.5 ml i.p. de veneno (4 μg.ml-1). El número de muertes fue contabilizado a las 24 y 48 h posteriores a la inoculación, demostrándose que la capacidad neutralizante de los anticuerpos IgG anti-PLA2 fue superior a la obtenida con el antiveneno crotálico. Los resultados obtenidos demuestran la potencial aplicación de antivenenos constituidos por anticuerpos específicos contra PLA2, y/o la inclusión de estos anticuerpos como suplementos en antivenenos polivalentes. 2023-03-03T19:18:50Z 2023-03-03T19:18:50Z 2006 Artículo Rodríguez, Juan Pablo, et al., 2006. Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom. Medicina. Buenos Ares: Fundación Revista Medicina, vol. 66, no. 6, p. 512-516. ISSN-e 1669-9106. 0025-7680 http://repositorio.unne.edu.ar/handle/123456789/51204 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 512-516 application/pdf Fundación Revista Medicina Medicina, 2006, vol. 66, no. 6, p. 512-516.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Crotalus durissus terrificus
Phospholipase A2
Antibodies IgG
Neutralization
Fosfolipasa A2
Anticuerpos IgG
Neutralización
spellingShingle Crotalus durissus terrificus
Phospholipase A2
Antibodies IgG
Neutralization
Fosfolipasa A2
Anticuerpos IgG
Neutralización
Rodríguez, Juan Pablo
De Marzi, Mauricio César
Maruñak, Silvana Licia
Malchiodi, Emilio Luis
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom
topic_facet Crotalus durissus terrificus
Phospholipase A2
Antibodies IgG
Neutralization
Fosfolipasa A2
Anticuerpos IgG
Neutralización
description Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75). Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 μg) with Freund adjuvant. Groups of six mice (20 + 2 g) were inoculated with 0.5 ml i.p. of C. d. t. venom (4 μg) or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.
format Artículo
author Rodríguez, Juan Pablo
De Marzi, Mauricio César
Maruñak, Silvana Licia
Malchiodi, Emilio Luis
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
author_facet Rodríguez, Juan Pablo
De Marzi, Mauricio César
Maruñak, Silvana Licia
Malchiodi, Emilio Luis
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
author_sort Rodríguez, Juan Pablo
title Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom
title_short Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom
title_full Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom
title_fullStr Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom
title_full_unstemmed Rabbit IgG antibodies against phospholipase A2 from crotalus durissus terrificus neutralize the lethal activity of the venom
title_sort rabbit igg antibodies against phospholipase a2 from crotalus durissus terrificus neutralize the lethal activity of the venom
publisher Fundación Revista Medicina
publishDate 2023
url http://repositorio.unne.edu.ar/handle/123456789/51204
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