Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina

Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this w...

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Autores principales: Acosta, Ofelia Cristina, Leiva, Laura Cristina Ana, Peichoto, María Elisa, Maruñak, Silvana Licia, Ruiz, Raquel Mónica, Teibler, Gladys Pamela, Gay, Claudia Carolina, Rey, Laura
Formato: Artículo
Lenguaje:Inglés
Publicado: Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas 2022
Materias:
Philodryas olfersii
Edematogenic activity
Myotoxic activity
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/49815
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-49815
record_format dspace
spelling I48-R184-123456789-498152024-10-23T10:49:26Z Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina Acosta, Ofelia Cristina Leiva, Laura Cristina Ana Peichoto, María Elisa Maruñak, Silvana Licia Ruiz, Raquel Mónica Teibler, Gladys Pamela Gay, Claudia Carolina Rey, Laura Philodryas olfersii Edematogenic activity Myotoxic activity Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 ml of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 ml of PBS. Two hours after injection mice were killed by cervical dislocation and both feet were cut off and weighed individually. For the myotoxic activity mice were injected i.m. with 100 ml of solutions containing 40 mg of venom. Blood samples were extracted after 1, 3, 6, 8, 10, 12, 14, 16 and 24 h of venom injection to determinate serum CPK activity and mice were sacrif iced at the same time intervals to obtain the inoculated gastrocnemius muscle. They were f ixed with Bouin solution and stained with Hematoxylin-Eosin. Results showed that P. olfersii venom exhibits a high edematogenic activity (MED = 0.31 mg) and a moderate myotoxic activity. Myonecrosis reached its highest level after 12 h of venom injection as shown by plasmatic CPK levels (5,401 ± 330 IU/l) and microscopic assay. It demonstrates the potential toxicity of the venom of P. olfersii, who inhabits the North-East region of Argentina. It also reinforces the original warning concerning the potential danger of bites by colubrids. 2022-08-11T19:21:11Z 2022-08-11T19:21:11Z 2003 Artículo Acosta, Ofelia, et al., 2003. Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina. BIOCELL. Mendoza: Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas, vol. 27, no. 3, p. 363-370. e-ISSN 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/49815 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas BIOCELL, 2003, vol. 27, no. 3, p. 363-370.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Philodryas olfersii
Edematogenic activity
Myotoxic activity
spellingShingle Philodryas olfersii
Edematogenic activity
Myotoxic activity
Acosta, Ofelia Cristina
Leiva, Laura Cristina Ana
Peichoto, María Elisa
Maruñak, Silvana Licia
Ruiz, Raquel Mónica
Teibler, Gladys Pamela
Gay, Claudia Carolina
Rey, Laura
Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina
topic_facet Philodryas olfersii
Edematogenic activity
Myotoxic activity
description Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 ml of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 ml of PBS. Two hours after injection mice were killed by cervical dislocation and both feet were cut off and weighed individually. For the myotoxic activity mice were injected i.m. with 100 ml of solutions containing 40 mg of venom. Blood samples were extracted after 1, 3, 6, 8, 10, 12, 14, 16 and 24 h of venom injection to determinate serum CPK activity and mice were sacrif iced at the same time intervals to obtain the inoculated gastrocnemius muscle. They were f ixed with Bouin solution and stained with Hematoxylin-Eosin. Results showed that P. olfersii venom exhibits a high edematogenic activity (MED = 0.31 mg) and a moderate myotoxic activity. Myonecrosis reached its highest level after 12 h of venom injection as shown by plasmatic CPK levels (5,401 ± 330 IU/l) and microscopic assay. It demonstrates the potential toxicity of the venom of P. olfersii, who inhabits the North-East region of Argentina. It also reinforces the original warning concerning the potential danger of bites by colubrids.
format Artículo
author Acosta, Ofelia Cristina
Leiva, Laura Cristina Ana
Peichoto, María Elisa
Maruñak, Silvana Licia
Ruiz, Raquel Mónica
Teibler, Gladys Pamela
Gay, Claudia Carolina
Rey, Laura
author_facet Acosta, Ofelia Cristina
Leiva, Laura Cristina Ana
Peichoto, María Elisa
Maruñak, Silvana Licia
Ruiz, Raquel Mónica
Teibler, Gladys Pamela
Gay, Claudia Carolina
Rey, Laura
author_sort Acosta, Ofelia Cristina
title Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina
title_short Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina
title_full Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina
title_fullStr Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina
title_full_unstemmed Edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of Argentina
title_sort edematogenic and myotoxic activities of the duvernoy’s gland secretion of philodryas olfersii from the north-east region of argentina
publisher Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/49815
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