Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)

The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent assay (I-ELISA) and the competitive enzyme-linked immunosorbent assay (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. The V1 of I-ELISA identifies them through the...

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Autores principales: Martínez, Diana Elina, Aguirre, Nerina, Cipolini Galarza, María Fabiana, Torioni de Echaide, Susana Marta
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad Federal de Santa María 2022
Materias:
Brucellosis
Buffaloes
Serological diagnosis
Brucelose
Búfalos
Diagnóstico sorológico
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/48468
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-48468
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Brucellosis
Buffaloes
Serological diagnosis
Brucelose
Búfalos
Diagnóstico sorológico
spellingShingle Brucellosis
Buffaloes
Serological diagnosis
Brucelose
Búfalos
Diagnóstico sorológico
Martínez, Diana Elina
Aguirre, Nerina
Cipolini Galarza, María Fabiana
Torioni de Echaide, Susana Marta
Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
topic_facet Brucellosis
Buffaloes
Serological diagnosis
Brucelose
Búfalos
Diagnóstico sorológico
description The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent assay (I-ELISA) and the competitive enzyme-linked immunosorbent assay (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. The V1 of I-ELISA identifies them through the monoclonal (M23) anti-bovine IgG (I-ELISAM23) and the V2 through the ProteinA / G (I-ELISA-A/G). Serum samples of 862 buffaloes (Bubalus bubalis) from the Northeast of Argentina (NEA) were analyzed using the complement fixation test (CFT) as the reference. Receiving Operator Characteristic (ROC) analysis defined for the area under the curve (AUC) determined the cutoff points, sensitivity (Se) and specificity (Sp) for each test. CFT identified 107 positive and 755 negative sera. The best AUC (0.986), Concordance with CFT (96.3%) and kappa value (0.843) was obtained by I-ELISA A/G test. This assay showed the highest Se (95.33%) and C-ELISA the highest Sp (97%). FPA failed to measure the antibodies in 23 (2.65%) serum samples due to unsuccessful reading. I-ELISA M23 proved to be ineffective to diagnose brucellosis in bubaline sera. The four serological tests showed cutoff points lower than those standardized for bovines. As conclusion, I-ELISA A/G, C-ELISA and FPA with its limitations would be effective techniques for the diagnosis of brucellosis in buffaloes in the NEA, requiring an appropriate cut-off point to guarantee their maximum performance in this species.
format Artículo
author Martínez, Diana Elina
Aguirre, Nerina
Cipolini Galarza, María Fabiana
Torioni de Echaide, Susana Marta
author_facet Martínez, Diana Elina
Aguirre, Nerina
Cipolini Galarza, María Fabiana
Torioni de Echaide, Susana Marta
author_sort Martínez, Diana Elina
title Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
title_short Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
title_full Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
title_fullStr Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
title_full_unstemmed Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
title_sort fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (bubalus bubalis)
publisher Universidad Federal de Santa María
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/48468
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